The ultrastructure of the gingival epithelial layer in adults

Authors

DOI:

https://doi.org/10.26641/1997-9665.2026.2.12-20

Keywords:

Gums, gingival epithelium, ultrastructure, keratinocytes, transmission electron microscopy, keratinization

Abstract

Relevance. Periodontal diseases remain one of the most common problems in modern dentistry, which necessitates a detailed study of the morphofunctional state of the gums as the first biological barrier against pathogenic factors. Detailing the norms of the ultrastructural structure of the gums in adults is the foundation for studying the evolution of pathological processes and developing criteria for evaluating the effectiveness of the latest protocols for treating gum disease. The aim was to study the features of the ultrastructural organisation of cell components of the epithelial layer of the gums in adults. Methods. Transmission electron microscopy was used to study the soft tissues of the periodontium in clinically healthy individuals. Results and conclusion. A gradient increase in the electron density of keratinocytes was detected in the direction from the basement membrane to the surface layers, reflecting the dynamics of specific cellular metamorphosis. These changes are determined by the morphofunctional heterogeneity of the ultrastructural organisation of cells in different layers of the gingival epithelium. The cells of the basal layer are characterised by their high functional activity, as evidenced by the presence of a well-developed synthetic apparatus. In the cells of the spinous layer, against the background of a decrease in synthetic functions, a clearly formed apparatus of intercellular connections is observed. Epithelial cells of the granular layer are characterised by the onset of keratinisation processes and preparation for programmed cell death. The architectonics of the stratum corneum is the determined result of a cascade of metabolic and structural changes occurring in the keratinocytes of the basal, spinous and granular layers.

References

  1. Herz MM, Schamuhn J, Krumm B, Bartha V. Student-performed periodontal therapy: retrospective cohort study on outcomes and related recommendations for enhancing undergraduate periodontal education. BMC Med Educ. 2025;25(1):1130. doi: 10.1186/s12909-025-07699-2
  2. Fik VB, Krynytskyi RP, Dudok OV. [Pathomorphological changes in periodontal tissues under experimental chronic opioid exposure]. Morphologia. 2025;19(2):54-8. Ukrainian. DOI: doi: 10.26641/1997-9665.2025.2.54-58
  3. Celeste RK, Augustsson E., Bomfim RA, Agahi N, Haas AN, Fritzell J. Trends in Periodontitis by Socioeconomic Status in Brazil: National Surveys 1986–2023. Journal of Clinical Periodontology. 2026;53(6):901–10. doi: 10.1111/jcpe.70130.
  4. Luthra S, Orlandi M, Hussain SB, et al. Treatment of periodontitis and C-reactive protein: A systematic review and meta-analysis of randomized clinical trials. J Clin Periodontol. 2023;50(1):45-60. doi: 10.1111/jcpe.13709
  5. Sanz M, Herrera D, Kebschull M, et al. Treatment of stage I-III periodontitis-The EFP S3 level clinical practice guideline. J Clin Periodontol. 2020;47 (Suppl 22):4-60. doi: 10.1111/jcpe.13290
  6. Mulish M, Welsh U. Romeis. Mikroskopische technik. 19 Auflage. Heidelberg; 2015:87-98.
  7. Glauert AM. Recent advances of high voltage electron microscopy in biology. J Microsc. 1979;117(1):93-101. doi: 10.1111/j.1365-2818.1979
  8. Hayat MA. Principles and techniques of electron microscopy: biological applications. 4th ed.Cambridge: Cambridge University Press; 2000. doi: 10.1006/anbo.2001.1367
  9. Riznyk S, Riznyk Yu. Structural and functional disorders of gingival microcirculation in generalized periodontitis. Actual Problems of Medicine and Pharmacy. 2023;4(2):1–10. doi: 10.52914/apmp.v4i2.56
  10. Lutsyk OD, Chaikovskiy YuB, Yashchenko AM et.al., authors; Lutsyk OD, Chaikovskiy YuB, editor: Histolohiya. Tsytolohiya. Embriolohiya [Histology. Cytology. Embryology]. Vinnytsia: Nova Knyha; 2024. 496 p. Ukrainian.
  11. Mazur IP, Belyakov YuA, Khaidar GK. Klinichna parodontolohiya: suchasni aspekty. [Clinical periodontology: modern aspects]. Kyiv: Kniga-plus; 2023. 536 p. Ukrainian.
  12. Mescher A. L. Junqueira's Basic Histology: Text and Atlas. 17th ed. McGraw Hill; 2024. 576 p.
  13. Ushakova GO, editor. [Molecular mechanisms of intercellular communication]. Monograph. Dnipro: LIRA; 2018. 216 p. Ukrainian.
  14. Vitale I, Pietrocola F, Guilbaud E, et al. Apoptotic cell death in disease-Current understanding of the NCCD. Cell Death Differ. 2023;30(5):1097-154. doi: 10.1038/s41418-023-01153-w
  15. Anderton H, Alqudah S. Cell death in skin function, inflammation, and disease. Biochem J. 2022;479(15):1621-51. doi: 10.1042/BCJ20210606
  16. Skybchik OV, Kovalishin VI, Solomenchuk TM, Martovlos (Godovana) OI. [Ultrastructural changes in the gingival mucosa of patients with chronic generalized periodontitis and ischemic heart disease]. Modern dentistry. 2021;5:46-51. https://doi.org/10.33295/1992-576X-2021-5-46 Ukrainian.
  17. Jakobysak M, editor; Chop'yak V.V., translation from Polish. Immunology. Vinnytsia: Nova Knyha; 2004. 672 p. Ukrainian.
  18. Masna Z., Chelpanova I., Rudnytska Kh., Paltov Ye., Dudok O. Functional morphology of oral cavity orgsns. Dentomaxillary system. Galician Publishers LTD; 2024. 80 p. Ukrainian.
  19. Tacarada H. Ultrastructural studies of human gingiva. The lower part of the pocket epithelium in chronic periodontitis. J Periodont. 1974;45:155-69. doi: 10.1902/jop.1974.45.3.155

Published

2026-05-29

How to Cite

Zubachyk, V., Chelpanova , I., & Paltov , Y. (2026). The ultrastructure of the gingival epithelial layer in adults. Морфологія / Morphologia / Morfologìâ, 20(2), 12–20. https://doi.org/10.26641/1997-9665.2026.2.12-20

Issue

Section

Статті