Morphological characteristics of the lungs and assessment of specific blood immune parameters in guinea pigs during the course of experimental pneumonia

Authors

DOI:

https://doi.org/10.26641/1997-9665.2026.1.35-40

Keywords:

experimental pneumonia, T-lymphocytes, B-lymphocytes, circulating immune complexes, immune system

Abstract

Pneumonia is an acute inflammatory lung disease. Despite extensive experimental and clinical research, it remains a significant medical challenge. Aim. To investigate the features of morphological changes in the lungs, as well as the levels of T- and B-lymphocytes and circulating immune complexes in the blood serum of guinea pigs during the development of experimental pneumonia (EP) on days 7, 14, and 21 of the experiment. Methods. An experimental pneumonia model was established in 39 guinea pigs weighing 180–230 g. The animals were anesthetized via chloroform inhalation in a desiccator, followed by combined intranasal and inhalation administration of Staphylococcus aureus within a specifically designed chamber. Morphological examination was performed to verify pulmonary inflammation, and blood T- and B-lymphocyte counts were determined across four experimental groups. Results and conclusion. The research findings demonstrated that during the progression of experimental pneumonia (days 7, 14, and 21), there was a statistically significant decrease in cellular immunity parameters, starting from the 7th day of the study. Changes in the humoral immune status, specifically blood B-lymphocyte levels, were also observed in the experimental animals. he progression of EP is characterized by a significant elevation of circulating immune complexes levels in the blood serum of guinea pigs on the specified experimental days; this, coupled with increased B-lymphocyte counts, suggests activation of the humoral immune response. At the observed time points (days 7, 14, and 21), experimental pneumonia is associated with a gradual decline in T-lymphocytes, indicating potential suppression of cellular immunity alongside a statistically significant increase in B-lymphocytes.

References

  1. Borodina OS. [Community-acquired uncomplicated pneumonia in children: relationship with cytokine response and physical development]. Achievements of biology and medicine. 2018;2:25–8. Ukrainian. doi: 10.7363/070206.
  2. Bereznyakov VI. [The state of cellular and humoral immunity in community-acquired pneumonia]. Health of the nation. 2022;1:107-10. Ukrainian. doi: 10.24144/2077-6594.1.1.2022.254649.
  3. Eshwara VK, Mukhopadhyay C, Rello J. Community-acquired bacterial pneumonia in adults: An update. Indian J Med Res. 2020;151(4):287–302. doi: 10.4103/ijmr.ijmr_1678_19.
  4. Smiyan OI., Horbasʹ VA. [Community-acquired pneumonia in school-age children: impact on the immune system]. Monograph. Sumy: Sumy State University; 2024. 111 p. Ukrainian. Available from: https://essuir.sumdu.edu.ua/handle/123456789/97611
  5. Reheda MS, Furdychko LO, Reheda-Furdychko MM, Reheda SM. [Peculiarities of lipid peroxidation processes and antioxidant system in the lungs in the early period of experimental pneumonia formation]. Achievements of clinical and experimental medicine. 2016;1:41-3. doi: 10.11603/1811-2471.2016.v25.i1.6013.
  6. Huang Y, Liu A, Liang L, et al. Diagnostic value of blood parameters for community-acquired pneumonia. Int Immunopharmacol. 2018;64:10–5. doi: 10.1016/j.intimp.2018.08.022.
  7. Lanks CW, Musani AI, Hsia DW. Community-acquired Pneumonia and Hospital-acquired Pneumonia. Med Clin North Am. 2019;103(3):487–501. doi: 10.1016/j.mcna.2018.12.008.
  8. Brands X, Haak BW, Klarenbeek AM, Otto NA, Faber DR, Lutter R, Scicluna BP, Wiersinga WJ, van der Poll T. Concurrent Immune Suppression and Hyperinflammation in Patients With Community-Acquired Pneumonia. Front Immunol. 2020;11:796. doi: 10.3389/fimmu.2020.00796.
  9. Reheda MS. [Experimental models of allergic alveolitis and pneumonia], Methodological guidelines. Lviv; 2024. 13 p. Ukrainian.
  10. Romanova YUH, Barnich II. [A modern view of methods of prevention and treatment of chronic generalized periodontitis]. Experimental and clinical dentistry. 2018;1(2):9-13. Ukrainian.
  11. Chuhay OO. [Influence of corvitin on humoral immunity indices in experimental pneumonia development]. Achievements of Clinical and Experimental Medicine. 2018;2:255-7. Ukrainian. doi: 10.11603/1811-2471.2018.v0.i2.8471.
  12. Kononova OV. [Indicators of the cellular immune system in patients with generalized periodontitis under conditions of psychoemotional stress]. Modern dentistry. 2019;1:42-5. Ukrainian. doi: 10.33295/1992-576X-2019-1-42-45.
  13. Reheda MS, Kolishetsʹka MA, Yurevych VR. [The influence of thiotriazoline on the change of immune system in the blood of guinea-pigs under the conditions of development of experimental bronchial asthma]. Medical and Clinical Chemistry. 2015;17(2):52-55. Ukrainian.
  14. European Convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasburg: Council of Europe. 1986;123:52. Available from: https://rm.coe.int/168007a67b.
  15. Directive 2010/63/EU of the European Par-liament and of the Council of 22 September 2010 on the Protection of Animals Used for Scientific Pur-poses. Off J Eur Union. 2010;53(L276):33–79. Available from: https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:276:0033:0079:en:PDF
  16. Chernushenko YeF, Kogosova LS. [immunology and immunopathology of lung diseases]. Kyiv: Zdorov'ya; 1981. 208 p. Ukrainian.
  17. Reheda MS, Lyubinetsʹ LA, Shchepansʹkyy VF. [Features of immune homeostasis in the dynamics of development of experimental bronchial asthma]. Bulletin of Scintific Research. 2018;2:133-5. Ukrainian. doi: 10.11603/2415-8798.2018.2.8745.
  18. Haskova V, Kaslik J, Matejckava M. [A new method for determining circulating immunocomplexes in human hearts]. Cas Lek Ces. 1977;116(14):436-7.
  19. Matsumoto Y, Aryal RP, Heimburg-Moli-naro J, et al. Identification and characterization of circulating immune complexes in IgA nephropathy. Sci Adv. 2022;8(43):eabm8783. doi: 10.1126/sciadv.abm8783.
  20. Hruzieva TS, Lekhan VM, Ohniev VA, Haliienko LI, Kriachkova LV, Palamar BI, et al. [Biostatistics]. Vinnytsia: New Book; 2020. 384 p. Ukrainian. ISBN 978-966-382-857-2.

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Published

2026-03-27

How to Cite

Ivankiv , O., Chelpanova , I., & Regeda , M. (2026). Morphological characteristics of the lungs and assessment of specific blood immune parameters in guinea pigs during the course of experimental pneumonia. Морфологія / Morphologia / Morfologìâ, 20(1), 35–40. https://doi.org/10.26641/1997-9665.2026.1.35-40

Issue

Vol. 20 No. 1 (2026)

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Статті

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