Features of the distribution of peanut lectin receptors on regenerated cells in rats with simulated fractures using implants made of different materials

Authors

DOI:

https://doi.org/10.26641/1997-9665.2025.3.158-161

Keywords:

experimental model, fracture, regenerate, implant, osteosynthesis

Abstract

Background. Lectin receptors include carbohydrate-containing biopolymer molecules of cells and extracellular matrix that are capable of selectively interacting with lectins to form lectin-receptor complexes. Due to these properties, lectins play an important role in cell recognition and intercellular interaction processes. Lectins are involved in inflammatory processes. Determining the histotopography of lectin receptors and its changes allows us to establish the dynamics of morphogenesis, immune response, and intercellular interactions in tissues. Objective. The aim of the study was to establish the characteristics and dynamics of the distribution of peanut lectin receptors on regenerated cells in rats with a modeled fracture using implants made of different materials. Methods. The study examined the regenerated tibia of 48 white rats. The animals were divided into 4 groups of 6 each. The experimental three groups included 36 rats with a simulated tibial fracture with intramedullary implantation of implants made of different materials (group 1 – carbon composite, group 2 – magnesium alloy MS-10, group 3 – medical stainless steel). Group 4 consisted of control rats that underwent incomplete transverse diaphyseal fracture modeling without intramedullary fixation. The study was conducted on days 7 and 14. The removed bones were fixed in 10% formalin with subsequent decalcification, dehydration in an ascending alcohol battery, and poured into paraffin. Histological preparations were made. To detect carbohydrate residues β-D-Gal receptors, a lectin histochemical reaction was performed with peanut lectin (PNA) produced by Lektintest (Lviv). The results were evaluated using a semi-quantitative method. Results and discussion. The nature of changes in the density of receptors for β-D-Gal residues in connective tissue elements of regenerated bone tissue in rats with intramedullary implantation of implants made of different materials was established. Results. The highest density of receptors for peanut lectin (PNA) was observed on lymphocytes and connective tissue cells of the regenerate in animals with intramedullary implantation of carbon-carbon composite and magnesium alloy, indicating a pronounced reaction from the immune system. Conclusions. The nature of changes in the density of receptors for β-D-Gal residues in connective tissue elements of bone tissue regenerate in rats with intramedullary implantation of different materials was established. The highest density of receptors to peanut lectin (PNA) was observed on lymphocytes and connective tissue cells of the regenerate in animals with intramedullary implantation of carbon-carbon composite and magnesium alloy, indicating a pronounced immune system response.

References

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Published

2025-12-08

How to Cite

Bohdanov , P. (2025). Features of the distribution of peanut lectin receptors on regenerated cells in rats with simulated fractures using implants made of different materials. Морфологія / Morphologia / Morfologìâ, 19(3), 158–161. https://doi.org/10.26641/1997-9665.2025.3.158-161

Issue

Vol. 19 No. 3 (2025)

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