Features of the ultrastructural organization of the aortic wall (experimental study)

Authors

DOI:

https://doi.org/10.26641/1997-9665.2025.3.143-148

Keywords:

rat, ultrastructure, morphology, aorta, hemodynamics, endothelial cells, hemomicrocirculatory bed.

Abstract

Relevance. The full-scale Russian-Ukrainian war has caused severe medical consequences, intensifying the need for comprehensive awareness within Ukraine and the global community regarding the scale of these challenges and pathways to overcome them, including scientific approaches. Vascular pathologies commonly encountered in daily medical practice, stemming from injuries and infectious diseases, pose a serious threat to patients' lives. A crucial prerequisite for effective diagnosis and treatment of vascular diseases is a thorough understanding of normal vascular anatomy. Objective. To study the features of the ultrastructural organization of the wall of the normal white rat aorta. Methods. The study material consisted of sexually mature male white rats (n=10), weighing 160-200 g. To achieve the stated objective and study the ultra-morphology of the normal rat aorta wall, the method of electron microscopy was used. Animals were euthanized by overdose of ether anesthesia, and material was collected. Ultrastructural examination was performed using a generally accepted methodology. Biopsies of the ascending aorta, aortic arch, and descending aorta served as the material for the study. Results. The conducted research allowed us to establish the ultrastructural morphological features of the white rat aorta wall structure and perform its comparative anatomical analysis with the human aorta. Conclusion. Despite the existence of numerous comparative studies dedicated to the normal anatomy of the aorta, the results of our research will help to systematize and supplement existing scientific information for further investigations by morphologists and clinicians regarding the microanatomy of the aorta, from the perspective of developing new methods for the diagnosis, prevention, and treatment of vascular pathologies.

References

  1. Zayachkivska O, Fass R., Coenen A, Gonkowski S, Liashenyk, Bekesevych, et al. Looking ahead to 2026. Proc Shevchenko Sci Soc Med Sci. 2025;1(77). doi: 10.25040/ntsh.
  2. Martín-Carro B, Donate-Correa J, Fernández-Villabrille S, Martín-Vírgala J, Panizo S, Carrillo-López N, Martínez-Arias L, Navarro-González JF, Naves-Díaz M, Fernández-Martín JL, Alonso-Montes C, Cannata-Andía JB. Experimental Models to Study Diabetes Mellitus and Its Complications: Limitations and New Opportunities. Int J Mol Sci. 2023;24(12):10309. doi: 10.3390/ijms241210309.
  3. European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Stientific Purposes. Strasburg: Council of Europe. 1986;123:52. Available from: https://rm.coe.int/ 168007a67b.
  4. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the Protection of Animals Used for Scientific Purposes. Off J Eur Union. 2010;53(L276):33–79.
  5. Glauert AM. Recent advances of high voltage electron microscopy in biology. J Microsc. 1979;117(1):93-101. doi: 10.1111/j.1365-2818.1979. tb00233.x
  6. Havrylenko YuV, Laiko AA, Syniachenko VV, Kyianytsia MA, Osadcha TM. [Morphofunctional features of tissues and blood in rats against the background of experimental diabetes mellitus]. Modern Pediatrics. Ukrainian. 2023;1(129):99-104. doi: 10.15574/SP.2023.129.99.
  7. Shmidt O, Prylutska K, Gritsay A. Aortic dissection as one of the leading causes of death in Marfan syndrome: a clinical case. ЕМ [Internet]. 2020;16(1):95-9. Available from: https://emergency.zaslavsky.com.ua/index.php/journal/article/ view/1216.
  8. Topchii II, Kirienko AN, Kirienko DA, Yakovtsova II, Gavriluk AA, Danyliuk SV, Ivakhno IV, Tovazhnianska VD. Features of endothelium morphological structure in kidney vessels, coronary arteries and aorta during chronic kidney disease. Wiad Lek. 2019;72(7):1269-73.
  9. .Ortug G, Ignak S, Ortug A. Characteristics of lingual papillae in diabetic rats. Morphology. 2018;102(339):250-4. doi: 10.1016/j.morpho.2018. 08.003.
  10. Zakharova VP, Siromakha SO, Roos-Hesselink JW, KravchenkoVI, Davydova YV, Lazoryshynets VV, Thoracic Aortic Aneurysm in Pregnancy: Morphological Analysis of 6 Cases. Pathologia [Internet]. 2021;18:356-364. Available from: https://pat.zsmu.edu.ua/article/view/242822.
  11. Нagan PG, Nienaber CA, Isselbacher EM, Bruckman D, Karavite DJ, Russman PL, et al. The international registry of acute aortic dissectional (IRAD): new insights into an old disease. JAMA. 2000;283(7):897-903. doi: 10.1001/ jama.283.7.897.
  12. Backer JDe, Haugaa KH, Hasselberg NE, Hosson M, Brida M, Castelletti S, et al. ESC Scientific Document Group, 2025 ESC Guidelines for the management of cardiovascular disease and pregnancy: Developed by the task force on the management of cardiovascular disease and pregnancy of the European Society of Cardiology (ESC) Endorsed by the European Society of Gynecology (ESG). European Heart Journal, 2025; doi: 10.1093/eurheartj/ ehaf193.
  13. Cury M, Zeidan F, Lobato AC. Aortic disease in the young: genetic aneurysm syndromes, connective tissue disorders, and familial aortic aneurysms and dissections. Int J Vasc Med. 2013;3:267215. doi: 10.1155/2013/267215.
  14. Wang L, Zhang J, Fu W, Guo D, Jiang J, Wang Y. Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection. J Vasc Surg. 2012;56(6):1698-709. doi: 10.1016/j.jvs.2012.05.084.
  15. Buja L, Zhao B, Vela D. et al. Pathobiology of Aortic Aneurysms and Dissections: Synthesis of Recent Investigations and Evolving Insights. JACC Adv. 2025;4(5). doi: 10.1016/j.jacadv.2025.101682.

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Published

2025-12-08

How to Cite

Tsytovskyi , M., & Dudok , O. (2025). Features of the ultrastructural organization of the aortic wall (experimental study). Морфологія / Morphologia / Morfologìâ, 19(3), 143–148. https://doi.org/10.26641/1997-9665.2025.3.143-148

Issue

Vol. 19 No. 3 (2025)

Section

Статті

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