Morphological changes in the renal corpuscle cortex after two-week administration of nalbuphine and after its withdrawal

Authors

DOI:

https://doi.org/10.26641/1997-9665.2025.2.12-15

Keywords:

kidneys, renal glomerulus. nalbuphine, rats

Abstract

Relevance. Narcotic analgesics (opioids) are widely used in medicine for pain relief in severe injuries, cancer, and the postoperative period. At the same time, their strong effect on the central nervous system and high risk of addiction pose difficult challenges for science and society. The use of opioids, both for medical reasons and in individuals who abuse narcotic drugs, is one of the causes of acute and chronic kidney damage, since most narcotic analgesics or their metabolites are excreted through the kidneys and lead to damage to their structure and function. The aim of our study was to determine changes in the renal corpuscle four weeks after discontinuation of nalbuphine after two weeks of its use. Methods. Histological preparations were prepared according to the generally accepted method. Staining was performed with hematoxylin and eosin. The experimental study was conducted on sexually mature male rats of the “Wistar” line divided into two experimental groups. Results. At the end of the second week of the experiment, with daily administration of nalbuphine, the following changes in the structural organization of the kidneys were observed: overflow with erythrocytes and expansion of the capillary loops of the glomeruli, their hypercellularity, expansion and edema of the mesangial matrix of the renal corpuscles. Four weeks after drug withdrawal, it was found that the renal glomeruli of the subcapsular nephrons were more affected than the renal corpuscles located in the intracortical and juxtamedullary regions of the cortex. The capillary loops of most renal glomeruli of the cortex were dilated and crowded with erythrocytes. The connective tissue of the outer leaf of the Shumlyansky-Bowman capsule of individual renal corpuscles is thickened in some places. The mesangial matrix is ​​expanded in some places, the number of mesangial and sometimes endothelial cells in individual renal corpuscles is slightly increased. Conclusion. Two weeks of nalbuphine use leads to changes in the renal corpuscle, which are still evident four weeks after drug withdrawal.

References

  1. Murugan R, Kellum JA. Acute kidney injury: what's the prognosis? Nat Rev Nephrol. 2011;7(4):209-217. doi: 10.1038/nrneph.2011.13.
  2. Chawla LS, Amdur RL, Amodeo S, et al. The severity of acute kidney injury predicts progression to chronic kidney disease. Kidney Int. 2011;79:1361-1369. doi: 10.1038/ki.2011.42
  3. Kondakov II, Topchiy II, Kiriyenko OM. Vplyv hlitserolu na funktsionalʹno-morfolohichni pokaznyky nyrok pry modelyuvanni hostroyi ta khronichnoyi nyrkovoyi nedostatnosti u shchuriv. Ukrayinsʹkyy zhurnal nefrolohiyi ta dializu. 2013;3:14-20. http://nbuv.gov.ua/UJRN/Uzhn_2013_3_3
  4. MallappallilM, Sabu J, Friedman EA, Salifu M. What Do We Know about Opioids and the Kidney? Int J Mol Sci. 2017;18(1): 223.doi: 10.3390/ijms18010223.
  5. Feng G, Luo Q, Guo E, Yao Y, Yang F, Zhang B, Li L. Multiple organ dysfunction syndrome, an unusual complication of heroin intoxication: A case report and review of literature. Int. J. Clin. Exp. Pathol. 2015;8(9):11826–11830.
  6. Schultz JJ, Hsu AK, Gross GJ. Ischemic preconditioning and morphine-induced cardioprotection involve the delta δ-opioid receptor in the intact rat heart. J. Mol. Cell Cardiol. 1997;29:2187–2195. doi: 10.1006/jmcc.1997.0454.
  7. Pepe S, van den Brink OW, Lakatta EG, Xiao RP. Cross-talk of opioid peptide receptor and β-adrenergic receptor signalling in the heart. Cardiovasc. Res. 2004;63:414–422. doi: 10.1016/j.cardiores.2004.04.022.
  8. Kuczeriszka M, Lipkowski AW, Sadowski J, Kompanowska-Jezierska E. An endomorphine analog ([D-Ala (2)]-Endomorphin 2, TAPP) lowers blood pressure and enhances tissue nitric oxide in anesthetized rats. Pharmacol. Rep. 2016;68:616-619. doi: 10.1016/j.pharep.2016.01.007.
  9. Mutoh J, Ohsawa M, Hisa H. Effect of naloxone on ischemic acute kidney injury in the mouse. Neuropharmacology. 2013;51:10-18. doi: 10.1016/j.neuropharm.2013.03.001.
  10. Priano LL, Vatner SF. Morphine effects on cardiac output and regional blood flow distribution in conscious dogs. Anesthesiology. 1981;55:236-243. doi: 10.1097/00000542-198109000-00009.
  11. Vilkhova IV. Biochemical parameters of blood serum during long-term opioid use and after its withdrawal. Bulletin of Problems of Biology and Medicine. 2022;2(164):126-134 doi: 10.29254/2077–4214–2022–2–1–164-126-134
  12. Vilkhova IV, Mateshuk-Vatseba LR, Logash MV. Changes in some biochemical parameters of blood serum in the case of two- and six-week use of therapeutic doses of opioid and in the early stages of drug withdrawal. Scientific Bulletin of Uzhgorod University. 2022;1(65):97-102. doi: 10.32782/2415-8127.2022.65.17
  13. European Convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasburg: Council of Europe. 1986;123:52.
  14. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the Protection of Animals Used for Scientific Purposes. Off J Eur Union. 2010;53(L276):33–79.
  15. Suvarna SK, Layton C, Bancroft GD. (Eds.). Bancroft's Theory and Practice of Histological Techniques, 8th Edition. Elsevier; 2019. 558 p. doi: 10.1016/B978-0-7020-6864-5.00008-6

Downloads

Published

2025-09-02

How to Cite

Vilkhova , I., & Podolyuk , M. (2025). Morphological changes in the renal corpuscle cortex after two-week administration of nalbuphine and after its withdrawal. Морфологія / Morphologia / Morfologìâ, 19(2), 12–15. https://doi.org/10.26641/1997-9665.2025.2.12-15

Issue

Vol. 19 No. 2 (2025)

Section

Статті

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.