Changes dynamics in the cornea layers of the rat eye at the end of the fourth week of experimental streptozocin diabetes

Authors

  • Kh.I. Rudnytska Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • M.I. Servetnyk Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • N.O. Ambarova Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • V.F. Simonov «Grace Clinic», Kyiv, Ukraine
  • A.P. Hrytsenko «Grace Clinic», Kyiv, Ukraine
  • I.V. Tverdokhlib Dnipro State Medical University, Dnipro, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2024.3.104-109

Keywords:

eye, cornea, experiment, pathology, diabetes, rat.

Abstract

Background. Ophthalmic complications of diabetes are still a huge medical and social problem worldwide. Taking into account the above, diabetic damage to the structures of the eyeball will continue to be an actual problem of the possible development of diabetic damage to the cornea. The aim of our study was to find out the features of the microstructural reconstruction of the rat cornea at the end of the fourth week of experimental streptozotocin-induced diabetes. Methods. Experimental diabetes was induced by a single intraperitoneal injection of streptozotocin at the rate of 7 mg per 100 g of body weight. Research was conducted from the fourth week of the experiment on animals with a glucose level of more than 15.58 mmol per 1 liter. 2 groups of animals were used in the work: 1 group (10 animals) with diabetes that developed (4 weeks after administration of streptozotocin); Group 2 was the control group (5 animals). Preparations for histological examination were prepared according to generally accepted methods. Microscopic studies and photography of the preparations were carried out using a MBI-1 microscope and a Nicon D 3100 digital camera. Results and conclusion. The outer epithelium was preserved in most areas, but alterative changes were registered in the cells of the basal, middle and superficial layers. The cells of the basal layer were located loosely. Epitheliocytes with vacuolated cytoplasm and compacted, homogeneously basophilic, pyknotic nucleus were found in the basal layer. Cells of the middle and superficial layers of the outer epithelium of the cornea were randomly arranged chaotically. Epitheliocytes with vacuolated cytoplasm were also found in these layers. As a result of dystrophic and necrotic changes in the cells of the basal and middle layers, there were foci of destructively changed epithelial cells in the layer of the outer epithelium of the cornea. In addition, in the back part of the corneal stroma, the matrix was unevenly colored, in separate plates it had a fine-grained acidophilic appearance. Keratocytes were located sparsely. Areas of edema also occurred between the connective tissue plates of the corneal stroma and Descemet's membrane. The endothelium of the anterior chamber of the eye was detached from Descemet's membrane in some areas. There were areas of corneal bulging where a decrease in the number of rows of cells in the middle and superficial layers was noted. Between the connective tissue plates of the corneal stroma and the Descemet's membrane, there were also areas of edema with detachment of the endothelium of the anterior chamber from the Descemet's membrane. The study of the structural rearrangement in the layers of the cornea at the end of the fourth week of experimental streptozotocin-induced diabetes will provide an opportunity to compare the depth of pathomorphological changes at different time points, which will further contribute to the creation of a pathomorphological basis for establishing the optimal terms from the point of view of morphology for drug correction of changes in the layers of the cornea in subchronic terms of exposure hyperglycemia in order to stabilize the emerging and subsequently progressing phenomena of diabetic keratitis.

References

  1. Fong DS, Aiello L, Gardner TW, et al. American Diabetes Association. Retinopathy in diabetes. Diabetes Care. 2004;27(1):84-87.
  2. Сheung N, Wong TY. Diabetic retinopathy and systemic vascular complications. Prog. Retin. Eye Res. 2008;27(2):161-176.
  3. Liew G, Wong TY, Mitchell P, et al. Retinopathy predicts coronary heart disease mortality. Heart. 2009;95(5):391-394.
  4. Vijan S, Hofer TP, Hayward RA. Cost-utility analysis of screening intervals for diabetic retinopathy in patients with type 2 diabetes mellitus. JAMA. 2000;283(7):889-896.
  5. Kempen JH, O’Colmain BJ, Leske MC, et al. Eye Diseases Prevalence Research Group. The prevalence of diabetic retinopathy among adults in the United States. Arch. Ophthalmol. 2004;122(4):552-563.
  6. Chang YS, Tai MC, Ho CH, Chu CC, Wang JJ, Tseng SH, Jan RL. Risk of Corneal Ulcer in Patients with Diabetes Mellitus: A Retrospective Large-Scale Cohort Study. Sci Rep. 2020;10(1):73-88.
  7. Wang B, Yang S, Zhai HL, Zhang YY, Cui CX, Wang JY, Xie LX. A comparative study of risk factors for corneal infection in diabetic and non-diabetic patients. Int. J. Ophthalmol. 2018;11(1):43-47.
  8. Badawi AE, Moemen D, El-Tantawy NL. Epidemiological, clinical and laboratory findings of infectious keratitis at Mansoura Ophthalmic Center, Egypt. Int. J. Ophthalmol. 2017;10(1):61-67.
  9. Hovind P, Tarnow L, Rossing K, et al. Decreasing incidence of severe diabetic microangiopathy in type 1 diabetes. Diabetes Care. 2003;26(4):1258-1264.
  10. Nordwall M, Bojestig M, Arnqvist HJ, et al. Diabetes Complications Study. Declining incidence of severe retinopathy and persisting decrease of nephropathy in an unselected population of type 1 diabetes. Diabetologia. 2004;47(7):1266-1272.
  11. Bloomgarden ZT, Drexler A. Fibrate use in diabetes. New concepts. J. Diabetes. 2011;3(1):1-2.
  12. Kronschläger MN, Talebizadeh YuZ, Meyer LM, Löfgren S. Apoptosis in Rat Cornea After In Vivo Exposure to Ultraviolet Radiation at 300 nm. Cornea. 2015;34(8):945–949. DOI: 10.1097/ICO.0000000000000498.
  13. Wilson SE. Corneal wound healing. Exp. Eye Res. 2020;197:108089. DOI: 10.1016/j.exer.2020.108089.
  14. Lovald ST, Rau A, Nissman S, et al. Finite element analysis and experimental evaluation of penetrating injury through the cornea. J. Mech. Behav. Biomed. Mater. 2017;66: 104–110. DOI:10.1016/j.jmbbm.2016.11.001.
  15. Belknap EB. Corneal Emergencies. Top Companion Anim. Med. 2015;30(3):74–80. DOI: 10.1053/j.tcam.2015.07.006.
  16. Slobodyan OM, Vovk OYu, Chelpanova I.V, Hnidyk YuV. [Morphofunctional features of the structural components of the eyeball in a comparative-species aspect and in various pathological processes (review article)]. Morphologia. 2022;16(3):7–14. Ukrainian. DOI: 10.26641/1997-9665.2022.3.7-14
  17. Barrientez B, Nicholas SE, Whelchel A, et al. Corneal injury: Clinical and molecular aspects. Exp. Eye Res. 2019;5:186. DOI: 10.1016/j.exer.2019.107709.
  18. Ebenezar OO, Roney A, Goswami DG, et al. Ocular injury progression and cornea histopathology from chloropicrin vapor exposure: Relevant clinical biomarkers in mice. Exp. Eye Res. 2023;230:109440. DOI: 10.1016/j.exer.2023.109440.
  19. Turchyn MV, Volkov KS, Klishch IM, Borys RM. [Histological and electron microscopic study of keratoxenoimplant with different types of preservation]. Zdobutky klinichnoyi i eksperymentalʹnoyi medytsyny. 2016;25(1):73-77. Ukrainian. DOI: 10.11603/1811-2471.2016.v25.i1.6033
  20. Horalskyi LP, Khomych VT, Kononskyi OI. [Basics of histological technique and morphofunctional research methods in normal and pathological conditions]. Zhytomyr: Polissia. 2015. 286 p. Ukrainian.

Downloads

Published

2024-10-30

How to Cite

Rudnytska , K., Servetnyk , M., Ambarova , N., Simonov , V., Hrytsenko , A., & Tverdokhlib , I. (2024). Changes dynamics in the cornea layers of the rat eye at the end of the fourth week of experimental streptozocin diabetes. Морфологія / Morphologia / Morfologìâ, 18(3), 104–109. https://doi.org/10.26641/1997-9665.2024.3.104-109

Issue

Vol. 18 No. 3 (2024)

Section

Статті

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.