Analysis of morphometric parameters of lung tissue under conditions of exposure to the venom of the vipers Vipera berus berus and Vipera berus nikolskii

Authors

DOI:

https://doi.org/10.26641/1997-9665.2024.4.61-65

Keywords:

vipers, lungs, venom, respiratory tract, rats.

Abstract

Background. According to the Ministry of Health of Ukraine, since the beginning of 2020, 36 cases of snake bites have been registered in Ukraine, including six children. Their significant prevalence is registered in the southern and eastern regions, and only one species is in the western and northern regions. The venom of vipers consists of about 25 proteins and peptides with enzymatic activity. The latter mainly exhibit hemotoxic effects, but neurotoxic, myotoxic, etc., have also been studied. Objective. Analysis of morphometric indicators of rat lung tissue under the influence of the venom of the vipers Vipera berus berus and Vipera berus nikolskii. Methods. Experimental studies were conducted on white, nonlinear male rats. The animals were conditionally divided into control and experimental groups, 10 individuals each. In saline, experimental rats were intraperitoneally injected with a semi-lethal dose (LD50) (1.576 mg·g-1) of Vipera berus berus and Vipera berus nikolskii venom. Control group animals were intraperitoneally injected with saline only. Rats were removed from the experiment 24 hours after exposure to the venom and euthanised by cervical dislocation. Morphometric studies were performed using a system for visual analysis of histological preparations. Images from histological preparations were displayed on a computer monitor from a MICROmed SEO SСAN microscope using a Vision CCD Camera video camera. Morphometric studies were performed using SEO ImageLabBio, ImageJ, and STATISTICA 10.0 programs. The studies were performed within the specified experimental periods in preparations stained with hematoxylin and eosin. Results and conclusion. The introduction of the venom of the viper Vipera berus berus to rats was accompanied by a significant increase in the average value of the area of blood vessels by 1.31 times compared to the group of intact animals. A significant decrease in the average value of the respiratory department was noted to 0.8. In this group of animals, the indicators of dysatelectasis, atelectasis and emphysematous lung tissue also significantly increased by 6.03, 7.15 and 2.0 times compared to the control rats. When exposed to the venom of the viper Vipera berus nikolskii, the experimental animals showed a significant increase in the average value of the area of blood vessels by 1.55 times compared to the group of intact rats. A significant decrease in the average value of the respiratory department was found to be 0.74. The relative areas of dysatelectasis, atelectasis, and emphysematous areas of lung tissue significantly increased by 6.64, 8.80, and 2.92 times compared to the control group.

References

  1. Cristina RT, Kocsis R, Tulcan C, Alexa E, Boldura OM, Hulea CI, et al. Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents. Braz J Med Biol Res. 2020;53(1):e9001. Doi: 10.1590/1414-431X20199001
  2. Hiu JJ, Yap MKK. Cytotoxicity of snake venom enzymatic toxins: phospholipase A2 anf L-amino acid oxidase. Biochem Soc Trans. 2020;48(2):719-731. Doi: 10.1042/BST20200110
  3. Jeon YJ, Kim JW, Park S, Shin DW. Risk factor, monitoring, and treatment for snakebite induced coagulopathy: a multicenter retrospective study. Acute Crit Care. 2019;34(4):269-275. Doi: 10.4266/acc.2019.00591
  4. Dyachenko IA, Murashev AN, Andreeva TV, Tsetlin VI, Utkin YN. Analysis of nociceptive effects of neurotoxic phospholipase A2 from Vipera nikolskii venom in mice. J Venom Res. 2014;4:1-4. PMID: 23577231
  5. Ferras CR, Arrahman A, Xie C, Casewell NR, Lewis RJ, Kool J, et al. Multifunctional toxins in snake venoms and therapeutic implications: from pain to hemorrhage and necrosis. Front Ecol Evol. 2019;7. https://doi.org/10.3389/fevo.2019.00218
  6. Malina T, Krecsák L, Westerström A, Szemán-Nagy G, Gyémánt G, M-Hamvas M, et al. Individual variability of venom from the European adder (Vipera berus berus) from one locality in Eastern Hungary. Toxicon. 2017;135:59-70. Doi: 10.1016/j.toxicon.2017.06.004
  7. Sartim MA, Souza COS, Diniz CRAF, da Fonseca VMB, Souza LO, Peti APF, et al. Crotoxin-induced mice lung impairment: role ofnicotinic acetylcholine receptors and COX-derived prostanoids. Biomolecules. 2020;10(5):794. Doi: 10.1016/j.ijbiomac.2017.05.146
  8. Tasoulis T, Isbister GK. A review and database of snake venom proteomes. Toxins (Basel). 2017;9(9):290. Doi: 10.3390/toxins9090290
  9. Megale ÂAA, Magnoli FC, Kuniyoshi AK, Iwai LK, Tambourgi DV, Portaro FCV, et al. Kn-Ba: a novel serine protease isolated from Bitis arietans snake venom with fibrinogenolytic and kinin-releasing activities. J Venom Anim Toxins Incl Trop Dis. 2018;24:38. Doi: 10.1186/s40409-018-0176-5

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Published

2025-01-15

How to Cite

Lasavutz , V. (2025). Analysis of morphometric parameters of lung tissue under conditions of exposure to the venom of the vipers Vipera berus berus and Vipera berus nikolskii. Морфологія / Morphologia / Morfologìâ, 18(4), 61–65. https://doi.org/10.26641/1997-9665.2024.4.61-65

Issue

Vol. 18 No. 4 (2024)

Section

Статті

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