Structural changes in the tendinous part of the rat diaphragm under conditions of jaundice and low-pressure pneumoperitoneum

Authors

DOI:

https://doi.org/10.26641/1997-9665.2024.4.45-51

Keywords:

jaundice, diaphragm, pneumoperitoneum, rats, histology.

Abstract

Background. Оbstructive jaundice is one of the most serious complications of diseases of the organs of the pancreaticoduodenal zone. Laparoscopy is widely used in surgical operations for jaundice and not only because of its minimally traumatic nature and contributes to the rapid recovery of the patient. In recent years, laparoscopic methods have significantly progressed in minimally invasive hepatobiliary and pancreatic surgery. However, there is still not enough data on how pneumoperitoneum caused by carbon dioxide, which is a companion of any laparoscopy, affects the abdominal organs. Objective of the work was to investigate changes in the tendon part of the diaphragm that occur in the presence of mechanical jaundice after the creation of pneumoperitoneum. Methods. The study was performed on 75 rats, divided into 5 groups. Mechanical jaundice was simulated by ligation of the common bile duct under anesthesia. Pneumoperitoneum was created using the KARL STORZ electronic laparoflator 264300 20 insufflator. The preparations were stained with hematoxylin and eosin. Results. In the intact group, the tendon part microscopically corresponded to the structure inherent to this tissue. Under the influence of jaundice, structural disorders occurred: the thickness increased, which was due to edematous loosening; collagen fibers had a tortuous appearance; increased cellular infiltration, both diffuse and with the formation of focal infiltrates. After 2 hours, edema increased and the unidirectionality of collagen fibers was disturbed. The presence of fatty tissue and macrophage and lymphocytic infiltrates was also monitored. After 3 hours, the compact arrangement of collagen fibers was partially preserved in the tendons, and over a larger area, collagen fibers were represented by an amorphous mucoid mass. Conclusion. In the presence of jaundice, looseness and tortuous course of collagen fibers with cellular infiltration in which macrophages were noted were noted. When pneumoperitoneum was used, deeper structural disorders were observed, which depended directly on the duration of intra-abdominal pressure.

References

  1. Poh BR, Cashin PA, Croagh DG. Impact of Jaundice on Outcomes Following Emergency Laparoscopic Cholecystectomy in Patients with Choledocholithiasis. World J Surg. 2018;42(10):3158-3164. doi: 10.1007/s00268-018-4588-8. PMID: 29541824
  2. Pavlidis ET, Pavlidis TE. Pathophysiological consequences of obstructive jaundice and perioperative management. Hepatobiliary Pancreat Dis Int. 2018;17(1):17-21. doi: 10.1016/j.hbpd.2018.01.008. Epub 2018 Jan 31. PMID: 29428098
  3. Poh BR, Ho SP, Sritharan M, Yeong CC, Swan MP, Devonshire DA, Cashin PA, Croagh DG. Randomized clinical trial of intraoperative endoscopic retrograde cholangiopancreatography versus laparoscopic bile duct exploration in patients with choledocholithiasis. Br J Surg. 2016;103(9):1117-1124. doi: 10.1002/bjs.10207. Epub 2016 Jun 15. PMID: 27302483
  4. Li J, Zhuo S, Chen B, Liu Y, Wu H. Clinical efficacy of laparoscopic modified loop cholecystojejunostomy for the treatment of malignant obstructive jaundice. J Int Med Res. 2020;48(2):300060519866285. doi: 10.1177/0300060519866285. Epub 2019 Sep 23. PMID: 31547725; PMCID: PMC7593670
  5. Muhammad WA, Talha Sh, Ashraf MA, Javaid J. Jaundice: a basic review. Int J Res Med Sci. 2016;(5):1313-1319. DOI: 10.18203/2320-6012.ijrms20161196
  6. Uygunovich NA. Effectiveness Mininvasive Laparoscopic Cholecystectomy during the Complication of Acute Cholecystitis. Research Journal of Trauma and Disability Studies. 2022;1(11):20–28.
  7. Kamalesh NP, Prakash K, Pramil K, George TD, Sylesh A, Shaji P. Laparoscopic approach is safe and effective in the management of Mirizzi syndrome. J Minim Access Surg. 2015;11(4):246-250. doi: 10.4103/0972-9941.140216. PMID: 26622114; PMCID: PMC4640023
  8. Koo JGA, Tham HY, Toh EQ, Chia C, Thien A, Shelat VG. Mirizzi Syndrome-The Past, Present, and Future. Medicina (Kaunas). 2023;60(1):12. doi: 10.3390/medicina60010012. PMID: 38276046; PMCID: PMC10818783
  9. Pavlovic Markovic A, Stojkovic Lalosevic M, Mijac DD, Milovanovic T, Dragasevic S, Sokic Milutinovic A, Krstic MN. Jaundice as a Diagnostic and Therapeutic Problem: A General Practitioner's Approach. Dig Dis. 2022;40(3):362-369. doi: 10.1159/000517301. Epub 2021 May 20. PMID: 34015787
  10. Aditianingsih D, Mochtar CA, Lydia A, Siregar NC, Margyaningsih NI, Madjid AS, Suwarto S. Effects of low versus standard pressure pneumoperitoneum on renal syndecan-1 shedding and VEGF receptor-2 expression in living-donor nephrectomy: a randomized controlled study. BMC Anesthesiol. 2020;20(1):37. doi: 10.1186/s12871-020-0956-7. PMID: 32019488; PMCID: PMC7001365
  11. Reznikov OG. [The fundamental ethical principles ofexperiments on animals]. Endocrinology. 2003;8(1):142-145. Ukrainian.
  12. Kritsak MYu, Rosolovska SO, Levchyk OI, inventors. Method for modeling experimental carboxyperitoneum in rats. Ukraine Certificate of copyright registration for the work UA 126409. 2024 May 16. Ukrainian.
  13. Kritsak MYu, Slabyi OB, Yasinovskyi OB, inventors. Method of selecting experimental material from the diaphragm of white rats. Ukraine Certificate of copyright registration for the work UA 126059. 2024 April 29. Ukrainian.
  14. Bahriy MM, Dibrova VA, Popadynets OH, Hryshchuk MI, autors; Bahriy MM, Dibrova VA, editors: Metodyky morfolohichnykh doslidzhen [Methods of Morphological Research]. Vinnytsia: A new book; 2016. 328 р. Ukrainian.

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Published

2025-01-15

How to Cite

Kritsak , M., Slabyi , O., & Yasinovskyi , O. (2025). Structural changes in the tendinous part of the rat diaphragm under conditions of jaundice and low-pressure pneumoperitoneum. Морфологія / Morphologia / Morfologìâ, 18(4), 45–51. https://doi.org/10.26641/1997-9665.2024.4.45-51

Issue

Vol. 18 No. 4 (2024)

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Статті

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