Markers of bone tissue remodeling in case of bone metabolism disorders

Authors

DOI:

https://doi.org/10.26641/1997-9665.2024.2.73-77

Keywords:

bone tissue, osteoblasts, osteoclasts, hyperglycemia, RANKL, osteopontin, metabolism.

Abstract

Introduction. Diabetes mellitus belongs to the group of debilitating metabolic diseases, as evidenced by the changes that occur in the organs and systems of the body. Today, experimental diabetology is of great importance for studying the pathogenesis of this disease. Functional and physiological restructuring of bone tissue occurs due to balanced processes of modeling and remodeling that run in parallel to each other. The article presents the results of the study of changes in biochemical markers of bone metabolism in rats with persistent hyperglycemia. The aim of the study was to determine the relationship between the level of metabolic changes in osteogenic cells and the secretion of bone remodeling markers in rat bones under conditions of experimental hyperglycemia. Methods. The study was conducted on 36 white laboratory rats. The rats were induced into a state of chronic hyperglycemia by a single intraperitoneal injection of alloxan dihydrate solution at a dose of 150 mg/kg body weight in 0.9% sodium chloride solution. The blood glucose level was determined by the glucose oxidase method. The level of glucose in urine was determined by the rapid method using test strips. The rats were withdrawn from the experiment every 30 days by decapitation under light thiopental-sodium anesthesia. The duration of the experiment was 60 days. Femurs were removed for the study. The study of osteogenic cells was performed by immunohistochemical analysis (IHC). The expression of osteopontin and RANKL in bone tissue was analyzed using a light microscope and the Allred scale. Statistical processing of the study results was performed using the Statistica v.10 program. Results. Persistent hyperglycemia leads to the development of destructive changes in osteogenic cells of the experimental group. These changes were manifested in a slowdown in bone remodeling, inhibition of osteogenic cells and their differentiation, which in turn leads to impaired growth and bone mineral density. In this case, in the rats of the experimental group, significant changes occurred already on day 30 of observation. Conclusion. It has been proved that in rats with a constantly high level of hyperglycemia there is a marked decrease in bone remodeling processes.

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Published

2024-07-30

How to Cite

Ponyrko , A., & Bumeіster V. (2024). Markers of bone tissue remodeling in case of bone metabolism disorders. Морфологія / Morphologia / Morfologìâ, 18(2), 73–77. https://doi.org/10.26641/1997-9665.2024.2.73-77

Issue

Vol. 18 No. 2 (2024)

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