Study of cytotoxicity and biocompatibility of isocyanurat-containing polyurethane foam filled with dacarbazine for application in medicine

Authors

DOI:

https://doi.org/10.26641/1997-9665.2024.2.44-54

Keywords:

isocyanurate-containing polyurethane foam, dacarbazine, culture of rat fibroblasts, implantation, biocompatibility.

Abstract

Background. The development of new and modification of existing polymer materials in order to obtain implants with improved functional capabilities that can perform the function of carriers of biologically active substances and drugs is an urgent task. Objective. Study of the biocompatibility of isocyanurate-containing polyurethane foam filled with dacarbazine by medical and biological methods. Methods. The study of cytotoxicity and biocompatibility of isocyanurate-containing polyurethane foams filled with dacarbazine was carried out using the rat fibroblast culture method in vitro and using the implantation test in vivo. Results. According to the results of research conducted using the rat fibroblast culture method, it was established that the culture of fibroblasts, both in the control and in the study of composite materials with different percentages of dacarbazine, was in the stage of stable growth, which indicated the absence of a cytotoxic effect of the experimental samples on the cultured cells. Using the implantation test, biocompatibility and the development of cellular reactions to the implantation of composite materials based on isocyanurate-containing polyurethane foam with dacarbazine in the body of experimental animals were studied. It was shown that the cellular reactions of the tissues of experimental animals to the implantation of test samples of isocyanurate-containing polyurethane foams of various compositions were typical for the reaction of a living organism to the presence of a foreign body during subcutaneous implantation and did not lead to pathological processes in the surrounding tissues, and the porous structure promoted the migration of cellular elements into the deeply implanted samples. Conclusion. According to the results of medical and biological studies, it is shown that isocyanurate-containing polyurethane foams with dacarbazine do not have a pronounced cytotoxic effect, are biocompatible and promising materials for use in medicine and are recommended for further tests, including clinical ones.

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Published

2024-07-30

How to Cite

Kuliesh , D., Rozhnova , R., Denisenko , V., Hrytsenko , V., Narazhaiko , L., & Galatenko , N. (2024). Study of cytotoxicity and biocompatibility of isocyanurat-containing polyurethane foam filled with dacarbazine for application in medicine. Морфологія / Morphologia / Morfologìâ, 18(2), 44–54. https://doi.org/10.26641/1997-9665.2024.2.44-54

Issue

Vol. 18 No. 2 (2024)

Section

Статті

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