Main morphometric parameters of the small and colon intestines in different functional states in rats

Authors

  • V.G. Gryn Poltava State Medical University, Poltava, Ukraine
  • V.P. Bilash Poltava State Medical University, Poltava, Ukraine
  • N.L. Svintsytska Poltava State Medical University, Poltava, Ukraine
  • R.L. Ustenko Poltava State Medical University, Poltava, Ukraine
  • S.I. Serbin Donetsk National Medical University, Lyman, Ukraine
  • A.L. Katsenko Poltava State Medical University, Poltava, Ukraine
  • A.V. Pilyugin Poltava State Medical University, Poltava, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2024.2.38-43

Keywords:

gastrointestinal tract, small intestine, large intestine, white rats, functional state.

Abstract

The aim of our study was to study the main morphometric parameters of the small and large intestine in different functional states in rats. Methods. The material for the study was 60 white rats of reproductive age, weighing 200.0±20.0 g, one half of which (n=30) were on daily fasting before vivisection, and euthanasia of the others (n=30) was performed immediately after morning feeding. First, after euthanasia by overdose of ether anesthesia, an autopsy and removal of the anterior abdominal wall of the abdomen was performed in all animals, followed by a total examination of the internal organs in their usual position. After photo-documentation of the internal organs, they proceeded to extract the total organ complex of the gastrointestinal tract from the abdominal cavity. Results. The direct distance between the pyloric sphincter and the ileocecal angle is quite small - approximately 35.0-40.0 mm, while the total length of the small intestine in some individuals of white rats reaches one meter with a thickness of about 3.0 mm. The general configuration of the loops of the small intestine, which are closely adjacent to each other, in each individual case after vivisection is presented differently due, most likely, to their peristaltic mobility. However, the individual variability of their shape cannot be rejected, which may depend, in turn, on the individual variable total length of the small intestine. In this regard, it should be noted that in the distal direction, i.e., as it approaches the ileocecal section, there is a gradual concentration of the food content, as a result of which its individual portions gradually turn into a mass that is relatively uniform in length. The task of our research was primarily to obtain basic metric data about the small and large intestine using the mathematical formula presented in the research materials and methods.

References

  1. Shevchenko KV, Yeroshenko GA, Yakushko OS. Morphometric description of the exchange segment of microvasculature of rats’ salivary glands in normal conditions and chronic ethanol intoxication. Wiadomości Lekarskie. 2019;72(3):323–326.
  2. Hryn VH, Kostylenko YuP, Yushchenko YuP. [General comparative anatomy of human and white rat digestive systems: a bibliographic analysis]. Anatomia porównawcza układu trawiennego człowieka i białego szczura. Analiza piśmiennictwa. Wiadomosci Lekarskie. 2018;LXXI(8):1599–1602. Polish.
  3. Hryn VH, Kostylenko YuP, Yushchenko YuP. [Comparative histological structure of the gastrointestinal mucosa in human and white rat: a bibliographic analysis]. Porównanie histologicznej struktury błony śluzowej przewodu pokarmowego człowieka i białego szczura: analiza bibliograficzna. Wiadomosci Lekarskie. 2018;LXXI(7):1398–1403. Polish.
  4. Shepitko KV, Shepitko VI. Morphometric characteristic of rat small intestine wall in administration of cryopreserved placenta and aseptic inflammation of peritoneum. Bulletin of problems of biology and medicine. 2016;2:380–385.
  5. Nagwa E. Effect of High Fat Diet on the Structure of the Ileum of Adult Female Albino Rat and The Role of Concomitant L-arginine Treatment. Light and Scanning Electron Microscopic Study. Egyptian Journal of Anatomy. 2016;39(2):1-17.
  6. Akimov OY, Kostenko VO. Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride. Ukr Biochem J. 2016;88(6):70-5. DOI: 10.15407/ubj88.06.070
  7. Herbert F Helander & Lars Fändriks. Surface area of the digestive tract – revisited. Scand J Gastroenterol. 2014;49(6):681-9. DOI: 10.3109/00365521.2014.898326.
  8. Pronina OM, Bilash SM, Kobeniak MM. Morphometric features of the structural components of the hemomicrocirculatory bed in the perivulnar region of the caecum in wound defect sutured with polyfilament suture material. Wiadomości Lekarskie. 2021;LXXIV(6). 1382–1388. DOI: 10.36740/WLek202106118.
  9. Nakaz Ministerstva osvity i nauky, molodi ta sportu Ukrayiny № 249 vid 01.03.2012 r. [Order of the Ministry of Education and Science, Youth and Sports of Ukraine No. 249 dated 01.03.2012]. Ofitsiynyy visnyk Ukrayiny. 06.04.2012;24:82. Ukrainian.
  10. Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes. 2010:276:0033:0079.
  11. Nakatsu N, Igarashi Y, Aoshi T, Hamaguchi I, Saito M, Mizukami T, et al. Isoflurane is a suitable alternative to ether for anesthetizing rats prior to euthanasia for gene expression analysis. The Journal of toxicological sciences. 2017;42(4):491-7. DOI: 10.2131/jts.42.491.
  12. Flecknell P. Chapter 1 - Basic Principles of Anaesthesia. In: Flecknell P, editor. Laboratory Animal Anaesthesia (Fourth Edition). Boston: Academic Press, 2016:1-75.
  13. Halʹchynsʹka OK. Veterynarna farmakolohiya: navchalʹnyy posibnyk. Kyiv: Ahrarna osvita, 2013. 525 p. Ukrainian.
  14. Patent 142955 Ukraine, МПК (2020.01) A61D 3/00 A61B 50/15 (2016.01). Operatsiyno-preparuvalʹnyy stolyk z fiksatoramy dlya laboratornykh shchuriv [Operating and preparation table with fixators for laboratory rats] / Hryn’ VH, Brovarnyk YaO. (UA); zayavnik i vlasnik patentu UMSA (UA). № u201911032; zayavl 08.11.2019 ; opubl 10.17.2020. Byul № 13/2020. Ukranian.
  15. Patent 147218 Ukraine, МПК (2021.01) G09B 23/28 (2006.01) A61D 1/00. Sposib preparuvannya totalʹnoho orhanokompleksu shlunkovo-kyshkovoho traktu u bilykh shchuriv [Preparation method of the total organocomplex of the white rats' gastroinestinal tract] / Hryn VH., Kostylenko YuP., Sherstyuk OO., Svintsytsʹka NL. (UA); zayavnik i vlasnik patentu UMSA (UA). № u202007050; zayavl 03.11.2020 ; opubl 22.04.2021. Byul № 16/2021. Ukranian.
  16. Ister OS. Samostiyni ta tematychni kontrolʹni roboty z alhebry ta heometriyi. Ternopilʹ: Navchalʹna knyha–Bohdan; 2019. 64 p. Ukrainian.
  17. Kaplun O. Matematyka. Formuly, ponyattya, vyznachennya. Kharkiv: Vydavnytstvo ULA; 2014. 192 p. Ukrainian.
  18. Fetisov VS. Paket statystychnoho analizu danykh STATISTICA : navch. posib. Nizhyn : NDU im. M. Hoholya; 2018. 114 p. Ukrainian.
  19. Tanavalee C, Luksanapruksa P, Singhatanadgige W. Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research. Clinical spine surgery. 2016;29(5):203-4. DOI: 10.1097/BSD.0000000000000382.
  20. Hryn VH. Makro-mikroskopicheskiye osobennosti rel'yefa slizistoy obolochki zheludochno-kishechnogo trakta belykh krys. [Macro-microscopic features of the relief of the mucous membrane of the gastrointestinal tract of white rats]. Svit medytsyny ta biolohiyi. 2019;70(4):188-93. DOI: 10.26724/2079-8334-2019-4-70-188-193. Russian.

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Published

2024-07-30

How to Cite

Gryn , V., Bilash , V., Svintsytska , N., Ustenko , R., Serbin , S., Katsenko , A., & Pilyugin , A. (2024). Main morphometric parameters of the small and colon intestines in different functional states in rats. Морфологія / Morphologia / Morfologìâ, 18(2), 38–43. https://doi.org/10.26641/1997-9665.2024.2.38-43

Issue

Vol. 18 No. 2 (2024)

Section

Статті

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