The effect of chronic administration of cadmium chloride on the morphological structures of the femu bone of rats

Authors

DOI:

https://doi.org/10.26641/1997-9665.2023.4.41-48

Keywords:

femur, morphology, bone tissue, cartilage tissue, density, heavy metals, cadmium chloride, morphometric indicators.

Abstract

Background. Cadmium, as a heavy metal, is part of many industrial wastes and can affect the environment and living organisms. One of the key systems exposed to cadmium is the skeletal system of humans and animals, particularly rats. The aim of the work was to reveal the specifics of the effect of chronic administration of cadmium chloride on the state of rat bones with a comparison of the effect of isolated administration of cadmium chloride on the morphofunctional state of the femur in a chronic experiment on rats. Methods. Histological and morphogistometric study. The results. In order to fulfill the set goal and ensure a correct comparative analysis of the obtained results of exposure to cadmium chloride during experimental studies, we studied the features of the morphogenesis of the femur in a control group of young male rats of puberty age in comparison to the group isolated exposure to cadmium chloride. Operative slaughter of animals to obtain samples took place on the 14th, 20th and 30th days of the experiment. After anesthesia, the bones were removed, weighed, measured for the protocol and fixed for further histological examination. Analyzing weight indicators, we determined the relative mass of the bone itself to the mass of the animal, which is more informative material for comparison between groups. Conclusion. Chronic exposure to cadmium chloride at a dose of 2.0 mg/kg in rats leads to a decrease in femur weight, which was confirmed by calculating the femur mass index. The introduction of cadmium chloride causes inhibition of growth processes in the femur and inhibition of osteogenesis, which is determined by the development of signs of dystrophic and osteoporotic changes in bone tissue in an experiment on rats. Osteometric measurements of all studied parameters of the femur and the results of histological examination of bone tissue confirm that cadmium chloride, which was chronically introduced into the body in excess, has a pronounced osteotropic effect. Cadmium chloride causes changes in the morphological structure of the articular cartilage of the femoral head. On the 30th day of the experiment, a significant number of resorptive changes were detected in the cartilage tissue, which manifested itself in numerous resorption cavities, disruption of the architecture of the radial layers of cartilage chondrocyte chains, and areas of connective tissue growth were observed.

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Published

2024-01-15

How to Cite

Nefodova , O., & Shevchenko , O. (2024). The effect of chronic administration of cadmium chloride on the morphological structures of the femu bone of rats. Морфологія / Morphologia / Morfologìâ, 17(4), 41–48. https://doi.org/10.26641/1997-9665.2023.4.41-48

Issue

Vol. 17 No. 4 (2023)

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