Morphological and morphometric studies of the structural components of the exocrine part of the pancreas of rats under the action of monosodium glutamate

Authors

DOI:

https://doi.org/10.26641/1997-9665.2023.4.27-33

Keywords:

pancreas, pancreatic acini, rats, monosodium glutamate, microscopic and morphometric study.

Abstract

Topicality. Experimental studies and clinical observations do not give an unequivocal answer about the safety of the monosodium glutamate food additive. Among body systems, the effect of monosodium glutamate on the pancreas is the least studied. The purpose. To determine the morphological and morphometric features of the structural changes in the components of the exocrine part of the pancreas of male rats after a long-term 8-week addition to the food of a food additive – monosodium glutamate. Methods. The experiment was conducted on 20 white male rats of reproductive age (2.5–3 months old, weighing 250.0±5.0 g). 70 mg/kg monosodium glutamate was added orally to the diet of 10 rats daily. Control rats (10 animals) received a standard diet. After 8 weeks, the rats were removed from the experiment and histological and morphometric studies were performed. Digital indicators are worked out statistically. Results. The use of monosodium glutamate by rats for 8 weeks led to pronounced structural changes in the exocrine part of the pancreas. The main disorders of the exocrine part include destructive and inflammatory changes, dilatation of intralobular and interlobular ducts. Exocrinocytes and acini of the pancreas are discomplexed. Between the acini, between the lobes and around the interlobular ducts, the amount of loose connective and adipose tissue increases. Violation of the structure of the acini is also associated with the expansion of the ducts and the formation of areas with edematous fluid. Vessels with signs of stasis were found in some areas of the pancreas. Pyknosis and lysis of exocrinocyte nuclei is noticeable in the acini of the pancreas. It was established that the average diameter of the acini in the experimental group of animals in comparison with the control rats significantly decreased by 25%. If in the control group of animals’ pancreatic acini with a diameter of 91–110 μm (17% of cases) and 111–130 μm (34% of cases) predominated, then in the experimental group 38% consisted of acini with a diameter from 31 to 90 μm. The diameter of the intercyanotic ducts increased significantly by 18.8%, intralobular ducts by 18.6%) and interlobular ducts by 12% compared to the control. Conclusion. Monosodium glutamate has a toxic effect on the exocrine part of the pancreas.

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Published

2024-01-15

How to Cite

Litvak , Y., Kochmar , M., Holovatskyi , A., Hetsko , O., & Zavadska , M. (2024). Morphological and morphometric studies of the structural components of the exocrine part of the pancreas of rats under the action of monosodium glutamate. Морфологія / Morphologia / Morfologìâ, 17(4), 27–33. https://doi.org/10.26641/1997-9665.2023.4.27-33

Issue

Vol. 17 No. 4 (2023)

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