Morphological changes in the skin of male and female rats after acute cold injury and correction of glucosa-mine hydrochloride

Authors

DOI:

https://doi.org/10.26641/1997-9665.2023.4.6-13

Keywords:

cold injury, morphology, skin, glucosamine, treatment, males, females, gender differences.

Abstract

Background. Cold injury leads to tissue necrosis as a result of both direct and indirect damage due to vasospasm and arterial thrombosis. The resistance of the body and the consequences of exposure to cold depend on individual factors (age, nutritional status, presence of diseases, use of medicines, physical activity, etc.). The gender factor in the development of cold injury and the body's response to treatment remains poorly understood. Glucosamine hydrochloride takes a prominent place among frigoprotective agents, but the gender determination of its frigoprotective action is currently unknown. Objective. To determine the peculiarities of the morphological changes of the skin in male and female animals against the background of acute cold injury and to evaluate the sexual dimorphism of the organoprotective effect of glucosamine under the given experimental conditions. Methods. Acute cold injury was modeled in sexually mature male and female rats by keeping them at -180C for 2 hours. The animals were divided into groups: I group - control; II group - male and female rats with acute cold injury without correction; Group III - animals of both sexes, which were injected intragastrically with glucosamine hydrochloride (50 mg/kg) 3 days before cold trauma modeling and for 3 days after it. The histological structure of the skin of the upper third of the tail was studied using a light microscope. Results. The histological layer-by-layer structure of the tail skin of male and female rats of the control group did not differ and had a normal histological structure. Acute cold injury caused significant dystrophic changes in the epidermis, dermis, and nerve fibers, impaired microhemocirculation of an ischemic nature, erythro- and leukostasis, microthrombosis, perivascular edema, and signs of endothelial dysfunction. These changes were more pronounced in male animals. Against the background of the introduction of glucosamine, the microscopic picture of damaged skin was significantly better, especially in males. The degree of expressiveness of pathological changes in animals of both sexes was lower, although signs of ischemia and hemostasis remained. Conclusion. Morphological changes in the skin of rats against the background of acute cold injury are more pronounced in male rats, glucosamine hydrochloride reduced the manifestations of the pathological condition in animals of both sexes, more signifcantly in males, while gender differences in microstructural disorders were significantly reduced and in some cases were removed.

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Published

2024-01-15

How to Cite

Voloshchuk , N., Yuhimchuk , A., & Shtrygol’, S. (2024). Morphological changes in the skin of male and female rats after acute cold injury and correction of glucosa-mine hydrochloride. Морфологія / Morphologia / Morfologìâ, 17(4), 6–13. https://doi.org/10.26641/1997-9665.2023.4.6-13

Issue

Vol. 17 No. 4 (2023)

Section

Статті

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