Changes in the structural organization of the thymus during extracellular dehydration

Authors

DOI:

https://doi.org/10.26641/1997-9665.2023.2.43-51

Keywords:

thymus, dehydration, thymocytes, epithelial reticular cells, endotheliocytes.

Abstract

Background. The functional state of the thymus affects the body's ability to protect against foreign antigens and the process of adaptation to adverse conditions. Objective. The purpose of the research was to study changes in the structural organization of the thymus during extracellular dehydration. Methods. Histological, immunohistochemical and electron microscopic research methods were used. The study was conducted on 30 adult male rats. The studied animals were divided into two groups. The experimental group of animals (15 animals) were subjected to extracellular dehydration, their diet included boiled food and bidistilled water for 60 days. In addition, during the experiment, the animals were injected intraperitoneally with Furosemide, its dose was 0.0003 g. Results. It was investigated that after 60 days from the beginning of the experiment in rats with an average degree of extracellular dehydration, accidental transformation of the III-IV phase was noted in most of the observations. The process of lymphocyte death is active, leading to the emptying of the medullary compartment and the loss of inversion of the substances. The number of plasma cells increases, and proliferating Ki-67+ cells decreases. The intercellular space contains areas of cellular detritus in the form of cell membranes, organelles, and chromatin. Some of all types of cells are characterized by the total destruction of membranes, nuclei, organelles of the cytoplasm, the evacuation of cytoplasmic material into the intercellular space and, ultimately, the formation of "cavities" - cyst-like vacuole-like structures. All vessels undergo changes in this term of the experiment. Full blood vessels and venous stasis are characteristic. The release of formed blood elements into the organ parenchyma is not a rare phenomenon. Conclusion. Therefore, after 60 days of extracellular dehydration, signs of accidental transformation of the III-IV phase develop.

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Published

2025-04-19

How to Cite

Prykhodko , O., & Prykhodko , E. (2025). Changes in the structural organization of the thymus during extracellular dehydration. Морфологія / Morphologia / Morfologìâ, 17(2), 43–51. https://doi.org/10.26641/1997-9665.2023.2.43-51

Issue

Vol. 17 No. 2 (2023)

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