Structural remodeling of retinal layers after two weeks of experimental opioid exposure

Authors

Keywords:

retina, opioid, two-week influence, experiment, rat.

Abstract

Background. Both domestic and international sources of specialized literature attach great importance to the study of the retina in its normal state and the processes of its structural changes under the influence of various factors and nosologies. Objective. The aim of the research is to determine the characteristics of pathological changes in the layers of the retinal tissue and its hemomicrocirculatory bed at the end of the 14th day of experimental opioid influence. Methods. The material of the study included 48 sexually mature male rats weighing 180-190 g and aged 4.5 months. The animals were injected intramuscularly with nalbuphine once daily. The initial dose of nalbuphine during the first 2 weeks was 0.212 mg/kg. The animals were withdrawn from the experiment by overdosing with diethyl ether. Ultrastructural preparations were prepared using a standard methodology. Vasodilation of the choroid vessels, reactive changes in the pigment epithelium, photoreceptors, bipolar cells, and ganglion cells were detected. In the photoreceptors, there was a narrowing of the cytoplasmic connection and disintegration of the membrane discs in the outer segments. Swelling and clearing of the perinuclear cytoplasm of the photoreceptors, as well as the cytoplasm of individual bipolar and ganglion cells, reactive changes in the processes of Müller cells, hyperemia of the choroid vessels, initial manifestations of degeneration of the pigment epithelium and destruction of the membrane discs in the outer segments of the photoreceptors, necrobiotic changes in the nuclei of the photoreceptors, degeneration of the axons of the outer plexiform layer and ganglion cells, and perivascular edema of the outer plexiform layer vessels were detected. Results of the conducted study indicate that under two weeks of opioid influence, circulatory changes occur and subsequently progress in the vascular networks of retinal layers, leading to the disruption of cell stratification within its layers against the background of gradually increasing edema.

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Published

2025-04-19

How to Cite

Paltov , Y. (2025). Structural remodeling of retinal layers after two weeks of experimental opioid exposure. Морфологія / Morphologia / Morfologìâ, 17(2), 36–42. Retrieved from https://morphology.dma.edu.ua/article/view/327210

Issue

Vol. 17 No. 2 (2023)

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Статті

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