Peculiarities of 3D reconstruction of the rudiments of skull bones in the prenatal period of human ontogenesis

Authors

DOI:

https://doi.org/10.26641/1997-9665.2023.3.131-139

Keywords:

3D reconstruction, intrauterine development, skull bones, anatomy, human.

Abstract

Background. 3D reconstruction of microscopic structures is a valuable method of morphological research of the early period of human ontogenesis. Much smaller than that of an adult organism, the size of the organs and structures of fetuses makes it technically possible to embedding them into a single paraffin block and reconstruct them in serial sections in anatomical continuity to study the form and syntopy. Objective is to find out the effectiveness of 3D reconstruction methods of bone tissue in the prenatal period of human development. Methods. The research was carried out on 6 series of consecutive histological sections of human embryos aged from 4 to 6 weeks of intrauterine development (IUD), 15 specimens of the head of human prefetuses aged from 7 to 12 weeks of IUD, human fetuses aged from 4 to 9 months of IUD by the method of creating histological (5), as well as histotopographic sections (10) directly from the paraffin block and their digitization, and 14 CT of human fetuses aged from 4 to 9 months of IUD. Results. 3D reconstruction of series of consecutive histological sections is effective for the study of embryo specimens, organocomplexes, in particular, regions of the head of prefetuses and human fetuses due to easy identification of histological structures, however, methods of comparing histological sections need to be improved. 3D-reconstruction of histotopographic sections is appropriate for the study of specimens of organocomplexes of human prefetuses and fetuses, allows identification of separate bone rudiments (cartilage tissue and ossification centers), as well as adjacent structures. 3D reconstruction of serial CT sections is effective for studying X-ray contrast anatomical structures in the fetal period of human development (bone tissue, contrasted blood vessels). Conclusion. The choice of the technique of 3D-reconstruction of embryological structures depends on the age period of the material for research: 3D-reconstruction of series of histological sections is advisable to use when studying embryos and prefetuses, as well as separate structures and organs of fetuses; 3D-reconstruction of a series of histotopographical sections – for the study of organ complexes of human fetuses and fetuses; 3D reconstruction of CT slices – for studying separate structures of human fetuses.

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Published

2023-10-31

How to Cite

Tsyhykalo , O., & Dmytrenko , R. (2023). Peculiarities of 3D reconstruction of the rudiments of skull bones in the prenatal period of human ontogenesis. Морфологія / Morphologia / Morfologìâ, 17(3), 131–139. https://doi.org/10.26641/1997-9665.2023.3.131-139

Issue

Vol. 17 No. 3 (2023)

Section

Статті

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