Morphometric and histochemical evaluation of leiomyocytes in the projection of the utero-placental area in iron-deficiency anemia of pregnancy

Authors

DOI:

https://doi.org/10.26641/1997-9665.2023.3.125-130

Keywords:

utero-placental area, leiomyocytes, chromotropophilia, protein oxidative modification, limited proteolysis, iron deficiency anemia of pregnancy.

Abstract

Background. With iron-deficiency anemia of pregnancy (IDA), the uteroplacental area (UPA) morphologically shows signs of gestational immaturity, there are prerequisites for a violation of hemodynamics in its projection and myometrial tone. Objective: to evaluate the morpho-functional state of leiomyocytes in the projection of UPA at IDA by establishing the quantitative characteristics of free radical damage. Methods. 74 biopsies of the myometrium in the UPA projection were studied during physiological pregnancy and gestation on the background of I, II and III degrees of IDA. By histochemical methods using the reaction with the chromotrope water-blue according to N.Z.Slinchenko, bromophenol blue for "acidic" and "basic" proteins according to Mikel Calvo and the ninhydrin-Schiff reaction for free amino groups of proteins according to A.Yasuma and T.Ichikava, in combination with computer microspectrophotometry and microdensitometry, quantitative parameters of free radical damage to leiomyocytes were established. Results. A significant increase in signs of free-radical damage to proteins was noted with IDA. The values of the studied parameters increased according to the degree of anemia and agreed with each other in the context of the interpretation of the phenomenon: specific volume of leiomyocytes in the state of chromotropophilia (Р<0.001), indicators of oxidative modification of proteins (Р<0.001) and limited proteolysis in the cytoplasm of muscle cells (Р<0.001) significantly exceeded the indicators of the physiological gestation group. Conclusions. 1. In cases of pregnancy with iron deficiency anemia, the degree of intensification of the processes of oxidative modification of proteins and limited proteolysis in the cytoplasm of leiomyocytes in the projection of the uteroplacental area correlates with the degree of severity of anemia. 2. Depending on the degree iron-deficiency anemia significantly affects the morpho-functional state of leiomyocytes in the projection of the utero-placental area creating prerequisites for the development of myometrial tone pathology, its contractile dysfunction and loss of myometrial control on the intensity of blood flow in the placental bed of the uterus.

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Published

2023-10-31

How to Cite

Tiulienieva , O., Davydenko , I., & Tiulienieva , V. (2023). Morphometric and histochemical evaluation of leiomyocytes in the projection of the utero-placental area in iron-deficiency anemia of pregnancy. Морфологія / Morphologia / Morfologìâ, 17(3), 125–130. https://doi.org/10.26641/1997-9665.2023.3.125-130

Issue

Vol. 17 No. 3 (2023)

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Статті

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