Connective tissue cells of the skin: heterogeneity of fibroblasts and general properties of their subpopulations

Authors

DOI:

https://doi.org/10.26641/1997-9665.2024.1.89-94

Keywords:

skin, fibroblasts, cell subpopulations, histophysiological properties.

Abstract

Directing of experimental and clinical research requires integration of information on morphological and functional features of organs and tissue structures. One of the examples of such integration is the analysis of the histophysiology of the skin, in particular dermal fibroblasts – a mandatory cellular component of the dermis. They not only synthesize and organize the components of the extracellular matrix of the dermis, but also interact with each other and with other types of cells, playing a decisive role in the regulation of skin histophysiology. Other resident cells include epidermal, vascular, and nerve cells. In addition, the skin contains various cells of hematogenous origin. They contain a constitutive population of dendritic cells and a more dynamic population of leukocytes, which includes monocytes (macrophages), neutrophils, and lymphocytes. Dermal fibroblasts are a dynamic and diverse population of cells whose functions in skin in many respects remain unknown. Normal adult human skin contains at least three distinct subpopulations of fibroblasts, which occupy unique niches in the dermis. Fibroblasts from each of these niches exhibit distinctive differences when cultured separately. Specific differences in fibroblast histophysiology are evident in papillary dermal fibroblasts, which reside in the superficial dermis, and reticular fibroblasts, which reside in the deep dermis. Both of these subpopulations of fibroblasts differ from the fibroblasts that are associated with hair follicles. Fibroblasts engage in fibroblast-epidermal interactions during hair development and in interfollicular regions of skin. They also play an important role in cutaneous structural transformations.

References

  1. Angel P, Szabowski A. Function of AP-1 target genes in mesenchymal-epithelial cross-talk in skin. Biochem Pharmacol. 2018;64:949-56.
  2. Detmar M. Molecular regulation of angiogenesis in the skin. J Invest Dermatol. 1996;106:207-8.
  3. Werner S, Smola H. Paracrine regulation of keratinocyte proliferation and differentiation. Trends Cell Biol. 2017;11:143-6.
  4. Nestle FO, Nickoloff BJ. A fresh morphological and functional look at dermal dendritic cells. J Cutan Pathol. 1995;22:385-92.
  5. Lugovic L, Lipozenocic J, Jakic-Razumovic J. Atopic dermatitis: immunophenotyping of inflammatory cells in skin lesions. Int J Dermatol. 2018;40:489-94.
  6. Jahoda CAB, Reynolds AJ. Hair follicle dermal sheath cells: unsung participants in wound healing. Lancet. 2019;358:1445-8.
  7. Sorrell JM, Baber MA, Caplan AI. Construction of a Bilayered dermal equivalent containing human papillary and reticular dermal fibroblasts: use of fluorescent vital dyes. Tissue Eng. 1996;2:39-49.
  8. Sorrell JM, Carrino DA, Baber MA. A monoclonal antibody which recognizes a glycosaminoglycan epitope in both dermatan sulphate and chondroitin sulphate proteoglycans of human skin. Histochem J. 1999;31:549-58.
  9. Akagi A, Tajima S, Ishibashi A. Expression of type XVI collagen in human skin fibroblasts: enhanced expression in fibrotic skin diseases. J Invest Dermatol. 1999;113:246-250.
  10. Tajima S, Pinnell SR. Collagen synthesis by human skin fibroblasts in culture: studies of fibroblasts explanted from papillary and reticular dermis. J Invest Dermatol. 1981;77:410-2.
  11. Moulin V, Auger FA, Garrel D, Germain L. Role of wound healing myofibroblasts on re-epithelialization of human skin. Burns. 2000;26:3-12.
  12. Marinkovich MP, Keene DR, Rimberg CS, Burgeson RE. Cellular origin of the dermal-epidermal basement membrane. Dev Dyn. 1993;197:255-67.
  13. Monical PL, Kefalides NA. Coculture modulates laminin synthesis and mRNA levels in epidermal keratinocytes and dermal fibroblasts. Exp Cell Res. 1994;210:154-9.
  14. Rubin JS, Bottaro DP, Chedid M. Keratinocyte growth factor. Cell Biol Int. 1995;19:399-411.
  15. Bernerd F, Asselineau D. Successive alteration and recovery of epidermal differentiation and morphogenesis after specific UVB-damages in skin reconstructed in vitro. Dev Biol. 1997;183:123-38.
  16. Szabowski A, Maas-Szabowski N, Andrecht S. c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cell. 2000;103:745-55.
  17. Andreadis ST, Hamoen KE, Yarmush ML, Morgan JR. Keratinocyte growth factor induces hyperproliferation and delays differentiation in a skin equivalent model system. FASEB J. 2001;15:898-906.
  18. Suzuki K, Yamanishi K, Mori O. Defective terminal differentiation and hypoplasia of the epidermis in mice lacking the Fgf10 gene. FEBS Lett. 2000;481:53-6.
  19. Kulessa H, Turk G, Hogan BLM. Inhibition of Bmp signaling affects growth and differentiation in the anagen hair follicle. EMBO J. 2000;19:6664-74.
  20. Botchkarev VA. Bone morphogenetic proteins and their antagonists in skin and hair follicle biology. J Invest Dermatol. 2003;120:35-47.
  21. Botchkarev VA, Botchkareva NV, Roth W. Noggin is a mesenchymally derived stimulator of hair-follicle induction. Nat Cell Biol. 1999;1:158-64.
  22. Millar SE. Molecular mechanisms regulating hair follicle development. J Invest Dermatol. 2002;118:216-25.
  23. Gailit J, Clark RAF. Wound repair in the context of extracellular matrix. Curr Opin Cell Biol. 1994;6:717-25.
  24. Adzick NS, Lorenz HP. Cells, matrix, growth factors, and the surgeon. The biology of scarless fetal wound repair. Ann Surg. 1994;220:10-8.
  25. Agren MS, Steenfos HH, Dabelsteen S. Proliferation and mitogenic response to PDGF-BB of fibroblasts isolated from chronic venous leg ulcers is ulcer-age dependent. J Invest Dermatol. 1999;112:463-9.
  26. Bernerd F, Asselineau D. UVA exposure of human skin reconstructued in vitro induces apoptosis of dermal fibroblasts: subsequent connective tissue repair and implications in photoaging. Cell Death Differ. 1998;5:792-802.
  27. Michel M, Germain L, Auger FA. Anchored skin equivalent cultured in vitro: a new tool for percutaneous absorption studies. In Vitro Cell Dev Biol Anim. 1993;29:834-7.
  28. Ponec M. Skin constructs for replacement of skin tissues for in vitro testing. Adv Drug Deliv Rev. 2002;54:19-30.
  29. Chang HY, Chi JS, Dudoit S. Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci USA. 2002;99:12877-82.

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Published

2024-04-29

How to Cite

Maievskyi, O., & Tverdokhlib, I. (2024). Connective tissue cells of the skin: heterogeneity of fibroblasts and general properties of their subpopulations. Морфологія / Morphologia / Morfologìâ, 18(1), 89–94. https://doi.org/10.26641/1997-9665.2024.1.89-94

Issue

Vol. 18 No. 1 (2024)

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Статті

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