Intracellular rearrangements of the rat contractile myocardial apparatus during prenatal ontogenesis after alcohol influence

Authors

DOI:

https://doi.org/10.26641/1997-9665.2024.1.56-61

Keywords:

prenatal ontogenesis, rats, alcohol intoxication, heart, ventricular myocardium, myofibrils, ultrastructure.

Abstract

Background. Information on the formation of the contractile apparatus of the heart and the distribution of myofibrils in cardiomyocytes under conditions of intrauterine intoxication with ethanol remain a subject of considerable debate. The aim of the study was to determine changes in the ultrastructure of the contractile apparatus of rat ventricular cardiomyocytes during prenatal development in conditions of intrauterine alcohol intoxication. Methods. The object of the research was the hearts of rat posterity at different times from birth to adulthood in the model of chronic alcohol intoxication of the maternal organism. Quantitative parameters of cardiomyocyte myofibrils in different zones of the ventricular myocardium were determined using transmission electron microscopy. Results. In newborn rats, after exposure to ethanol, the values of the parameters in the subendocardial zone were statistically significantly increased in the left ventricle by 101.0% and in the right ventricle by 42.0%, compared to the indicators of the previous day of development. Indicators in the intramural and subepicardial zones were not significantly different from the corresponding values on the 20th day of prenatal ontogenesis. The difference between the parameters of different parts of the interventricular septum was statistically significant and amounted to 45.9% in the left ventricular part and 20.2% in the right ventricular part. The values of the parameter in the intramural zone after ethanol exposure decreased by 35.5% (p>0.05) in the left ventricle and by 36.0% (p<0.05) in the right ventricle compared to the norm. Conclusion. Chronic alcohol intoxication in prenatal cardiogenesis damages the contractile apparatus of ventricular cardiomyocytes due to disorganization of the structure of sarcomeres, fragmentation and disorientation of myofibrils, significant inhibition of sarcomere genesis, and a decrease in the content of myofibrils, which is associated with destruction of mitochondria. The severity of changes in these structures depends on the zone and period of development of the embryo. The most significant changes are due to the direct toxic effect of ethanol and occur in the early stages of cardiogenesis.

References

  1. Avtandilov GG. Meditsinskaya morfometriya: rukovodstvo [Medical morphometry: guideline]. Moscow: Meditsina; 1990. 384 p. Russian.
  2. Ivanchenko MV, Tverdokhleb IV. Vliyaniye vnutriutrobnoy gipoksii na geterogenitet mitokhondriy i puti yego realizatsii pri al'teratsii zheludochkovogo miokarda krys [The influence of intrauterine hypoxia on the heterogeneity of mitochondria and the ways of its implementation during alteration of the ventricular myocardium of rats]. Vestnik Volgogradskogo gosudarstvennogo meditsinskogo universiteta. 2014;4(52):101-106. Russian.
  3. Ivanchenko MV, Tverdokhlib IV. Formuvannya mitokhondrialʹnoho aparata skorotlyvykh kardiomiotsytiv v normi ta za umov hipoksychnoho ushkodzhennya kardiohenezu [Formation of the mitochondrial apparatus of contractile cardiomyocytes in normal conditions and under conditions of hypoxic damage to cardiogenesis]. Morphologia. 2013;7(1):5-20. Ukrainian.
  4. Mashtalir MA, Tverdokhleb IV. Gistokhimicheskiye, lektingistokhimicheskiye i immunogistokhimicheskiye metody v embriologicheskom issledovanii serdtsa [Histochemical, lectinohistochemical and immunohistochemical methods in the embryological study of the heart]. Morphologia. 2010;4(2):39-44. Russian.
  5. Tverdokhleb IV, Shponka IS. Stereologicheskiye i lektinogistokhimicheskiye kharakteristiki morfogeneticheskikh mekhanizmov v serdtse mlekopitayushchikh [Stereological and lectinohistochemical characteristics of morphogenetic mechanisms in the mammalian heart]. Ukrainskyi morfologichnyi almanach. 1998;3:131-132. Russian.
  6. Tverdokhlib IV, Petruk NS, Ivanchenko MV, Silkina JuV, Khripkov IS, Pertseva NO, Shevchenko KM, Goodlett TO, Malkov II, Berehovenko IM, Zinenko DYu, Galaida NO, Varin VV, inventors; State Institution «Dnipropetrovsk medical academy of the Health Ministry of Ukraine». Method of determining the coordinates of ultrastructures in transmission electron microscopy of biological objects. Ukrainian patent UA 83611. 2013 Sep 25. Int. CI. G01N 1/28. Ukrainian.
  7. Conen D. Alcohol consumption and incident cardiovascular disease: not just one unifying hypothesis. Eur. Heart J. 2015;36(15):897–8.
  8. Drew NK, Grosberg A. Methods of myofibrillogenesis modeling. Methods Mol. Biol. 2015;1299:75–91.
  9. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg: Council of Europe. 18 Mar 1986. 53 p.
  10. Fenix AM, Neininger AC, Taneja N, Hyde K, Visetsouk MR, Garde RJ, Liu B, Nixon BR, Manalo AE, Becker JR. Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes. Elife. 2018 Dec 12;7. Epub 2018 Dec 12.
  11. Gardner JD, Mouton AJ. Alcohol effects on cardiac function. Compr. Physiol. 2015;5(2):791-802.
  12. Geach TJ, Hirst EM, Zimmerman LB. Contractile activity is required for Z-disc sarcomere maturation in vivo. Genesis. 2015;53(5):299-307.
  13. Hayat МА. Principles and techniques of electron microscopy: Biological applications [4th ed.]. Cambridge : Cambridge University Press; 2000. 543 p.
  14. Kamran K, Khan MY, Minhas LA. Atrial and ventricular septal changes in ethanol vapour exposed chick embryos. J. Pak. Med. Assoc. 2015;65(3):296–9.
  15. Kuo J. Electron microscopy: methods and protocols. Totowa, New Jersey : Humana Press Inc; 2007. 608 p.
  16. Sanger JW, Wang J, Fan Y, White J, Mi-Mi L, Dube DK, Sanger JM, Pruyne D. Assembly and Maintenance of Myofibrils in Striated Muscle. Handb Exp Pharmacol. 2017;235:39-75.
  17. Wang J, Fan Y, Sanger JM, Sanger JW. Nonmuscle myosin II in cardiac and skeletal muscle cells. Cytoskeleton (Hoboken). 2018;75(8):339-51.
  18. Zile MR, Baicu CF, Ikonomidis JS. Myocardial stiffness in patients with heart failure and a preserved ejection fraction: contributions of collagen and titin. Circulation. 2015;131(4):1247-59.
  19. Becker HC. Animal models of excessive alcohol consumption in rodents. Curr Top Behav Neurosci. 2013;13:355–377.

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Published

2024-04-01

How to Cite

Marchenko, D., & Tverdokhlib, I. (2024). Intracellular rearrangements of the rat contractile myocardial apparatus during prenatal ontogenesis after alcohol influence. Морфологія / Morphologia / Morfologìâ, 18(1), 56–61. https://doi.org/10.26641/1997-9665.2024.1.56-61

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Vol. 18 No. 1 (2024)

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