Morphological analysis of pathohistological changes in the liver after the traumatic effect of an air shock wave

Authors

  • S.V. Kozlov
  • M.A. Korzachenko

Keywords:

rat liver, injury, air shock wave, morphology, time of injury.

Abstract

Background. Closed blunt trauma to the abdomen, which is accompanied by liver injury of any degree, is life-threatening. Injury to the liver leads to a violation of numerous components of the synthesizing, detoxification, digestive and metabolic functions of the body. In this regard, the diagnosis and proper treatment of liver lesions is an extremely difficult task. Methods and results. In the presented study, pathohistological changes in the liver after exposure to an air shock wave at different times of the post-traumatic period were determined and evaluated. The material for the study was the liver of 30 male rats, weighing 177.5±15.8 g. All animals were randomly divided into three groups: 1 group consisted of intact rats (n-6), 2 - control (halothane anesthesia with fixation) (n -12), 3 – experimental animals (halothane anesthesia with fixation, traumatic single action of an air shock wave with an excess pressure of 31.6±4.8, which was generated in the device developed by us. After reproduction of the traumatic effect of the air shock wave, the morphological analysis of the liver parenchyma and stroma was performed on the 7th and 30th days by evaluating histological sections stained with hematoxylin and eosin. A week later, focal intraparenchymal and subcapsular hemorrhages, dystrophically changed hepatocytes, and neutrophilic infiltration in the periportal areas were found in the liver; on the thirtieth day, hemorrhages were no longer observed, inflammatory infiltration spread to intraparenchymal areas, foci of connective fibrous tissue were found in the liver. Conclusion. All detected pathohistological signs are secondary and can be used as markers of the time of injury formation and for the development of diagnostic pathohistological panels.

References

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Published

2022-06-15

How to Cite

Kozlov, . S. ., & Korzachenko , M. (2022). Morphological analysis of pathohistological changes in the liver after the traumatic effect of an air shock wave. Морфологія / Morphologia / Morfologìâ, 16(2), 31–37. Retrieved from https://morphology.dma.edu.ua/article/view/282014

Issue

Vol. 16 No. 2 (2022)

Section

Статті

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