Morphological characteristics of the blood-brain barrier in 1 day of experimental blast-induced traumatic brain injury

Authors

  • Yu.V. Kozlova
  • N.S. Tryasak
  • G.A. Klopotskyi
  • K.S. Kozlova

Keywords:

blood-brain barrier, explosion, brain, trauma.

Abstract

Background. Blast-induced traumatic brain injury is becoming widespread in connection with the use of explosives in military conflicts all over the world and today in Ukraine, which needs the elaboration of modern pathogenetically based treatment methods of the acute period and prevention of remote complications. Scientists have established that one of the significant primary injuries, which is the basis for the realization of secondary ones, in the case of a mild blast-induced traumatic brain injury, is a violation not only of neurons themselves, but also of the blood-brain barrier. Aim. Morphological determination of changes in the blood-brain barrier in 1st day of experimental blast-induced traumatic brain injury using a proprietary device for simulating blast injury. Methods. Brain sections of 12 albino male Wistar rats (body mass 220-270 g, age 6-7 months) were examined using light microscopy. Rats were randomly divided into 2 groups: I – Experimental group (n=6), the animals of which were anesthetized with Halothane, fixed with their heads to the muzzle end of a self-made and patented device at a distance of 5 cm and subjected to the action of a blast wave of 26-36 kPa; II - Sham (n=6). After 1 day, the rats of both groups were euthanized and brains were removed, which were then fixed and sections were prepared and stained with hematoxylin and eosin according to standard methods. Results. Established credible signs of blood-brain barrier disruption using our own device to reproduce a blast-induced traumatic brain injury, which are primary damages as a result of the action of the blast wave and are the basis for triggering secondary damage mechanisms and lead to neurodegeneration processes. Conclusion. The mild blast-induced traumatic brain injury after using a proprietary device was confirmed by morphological changes in the blood-brain barrier in the 1st day of the post-traumatic period in the form of ruptured and paretically dilated capillaries and desquamation of the vascular endothelium, the presence of erythrocyte aggregates. Increased permeability of blood vessels led to swelling of brain tissue and neurocytes.

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Published

2022-10-15

How to Cite

Kozlova, Y. ., Tryasak, N. ., Klopotskyi, G. ., & Kozlova, K. . (2022). Morphological characteristics of the blood-brain barrier in 1 day of experimental blast-induced traumatic brain injury. Морфологія / Morphologia / Morfologìâ, 16(3), 148–152. Retrieved from https://morphology.dma.edu.ua/article/view/282008

Issue

Vol. 16 No. 3 (2022)

Section

Статті

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