Cardiotoxic effect of lead acetate on heart morphogenesis based on the results of electron microscopy

Authors

  • I.V. Shevchenko
  • O.O. Nefedova
  • K.A. Kushnarova
  • О.V. Кuznetsova
  • V.G. Rutgaizer
  • O.V. Boyko

Keywords:

lead acetate, heart, myocardium, matrix, myofibril, cardiomyocytes, sarcomere, kristi, cardiotoxic effect of lead acetate.

Abstract

In modern society, changes in the environment that arise under the influence of anthropogenic factors, an increase in the number of salts of heavy metals, which are teratogens and can provoke disorders in the development of organs, are the object of interest. One such compound is lead acetate. Lead acetate has a high polytropic toxicity. The heart and the vascular system are sensitive to the effects of lead compounds, during the prenatal and postnatal period. Purpose. To study the ultrastructural manifestations of the cardiotoxic effect of lead acetate on the morphogenesis of the heart. Methods. On the 1st and 7th day after birth, rat pups were dissected and hearts were taken for electron microscopic examination. The research was carried out using a transmission electron microscope. Electronograms were obtained by taking ultra-thin sections on Agfa orthochromatic film. The diameter and bulk density of mitochondria, the length of sarcomeres of contractile myofibrils, and the bulk density of myofibrils were quantified. Results. The structure of the myocardium at the level of the right ventricle was investigated by the method of electron microscopy. A comparative analysis of the ultrastructure of cardiomyocytes and morphometric indicators, which may indicate dystrophic changes, was conducted. The decrease in the density of myofibrils in our own studies was detected already on the first day of the exposure of lead acetate. Changes in the density of myofibrils on day 7 were not detected, indicating a certain stability of these protein structures. At the same time, the length of sarcomeres was reduced. These changes should not be considered as a manifestation of the recovery process, since a significant number of myofibers have suffered damage. The general conclusion is an increase in the destructive changes in cardiomyocytes during the exposure of lead acetate. Conclusion. Cardiotoxic effects of lead acetate are manifested by swelling of cardiomyocytes and destructive changes in mitochondria and contractile myofibrils. The detected ultrastructural disorders are a consequence of the acute toxic effect of lead acetate.

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Published

2022-10-07

How to Cite

Shevchenko, I. . ., Nefedova, O. . ., Kushnarova, K. . ., Кuznetsova О. ., Rutgaizer, V. . ., & Boyko, O. . (2022). Cardiotoxic effect of lead acetate on heart morphogenesis based on the results of electron microscopy. Морфологія / Morphologia / Morfologìâ, 16(3), 133–138. Retrieved from https://morphology.dma.edu.ua/article/view/282004

Issue

Vol. 16 No. 3 (2022)

Section

Статті

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