Spatial-temporal distribution of expression of liver injury markers according to the results of immunohistochemical analysis after exposure to an air shock wave

Authors

Keywords:

rat liver, air shock wave, immunohistochemical analysis, eNOS.

Abstract

Background. The liver is one of the most vulnerable organs of the abdominal cavity after the blast wave. Diagnosis of closed liver injury, which is not accompanied by acute intra-abdominal blood loss, is not always effective. That's why, the search for liver damage markers after the blast wave action while preserving the integrity of the liver tissue at the time of damage, as well as at different times of the post-traumatic period, remains an urgent task. Considering this, the Aim of our study was to determine the spatio-temporal characteristics of the expression of liver damage markers after the experimental action of an air shock wave at different times of the post-traumatic period. Materials for the study was livers of 18 male rats, body mass 177.5±15.8 g. All animals were randomly divided into two groups: 1 group consisted of control rats (halothane anesthesia with fixation) (n -6), 2 – experimental animals (halothane anesthesia with fixation, traumatic single action of an air shock wave with an excess pressure of 31.6±4.8 (M ± SD), which was generated in the shock tube device) (n -12). After the simulation of the air shock wave traumatic effect, an immunohistochemical analysis was performed using the eNOS marker on the 7th and 30th day by qualitative and quantitative assessment of its expression in the liver tissue. Results. Immunohistochemical analysis in the experimental group of animals showed that in the early post-traumatic period (7th days) after exposure to an air shock wave, eNOS expression prevails in the central and intermediate zones of the liver lobules. In the late post-traumatic period (30th days), eNOS expression is characteristic of the periportal zones of the liver lobules. Conclusion. The revealed spatio-temporal heterogeneity of eNOS expression in the post-traumatic period indicates the phasing of adaptive-reactive changes associated with a different threshold of liver lobule cells resistance to primary structural disturbances after an air shock wave exposure.

References

  1. Saviano A, Ojetti V, Zanza C, Franceschi F, Longhitano Y, Martuscelli E, Maiese A, Volonnino G, Bertozzi G, Ferrara M, La Russa R. Liver trauma: management in the emergency setting and medico-legal implications. Diagnostics. 2022;12(6):1456. https://doi.org/10.3390/diagnostics12061456
  2. Kozlov SV, Tkachenko OV, Zrozhevskyi RS. [Forensic and medical characteristics of injuries caused by a contact explosion of a F-1 grenade]. Sudovo-medychna ekspertyza. 2016;(1):78-80. Ukrainian. DOI: https://doi.org/10.24061/2707-8728.1.2016.16.
  3. Mamedov ShM, Tkachenko OV, Kozlov SV, Vydysh KP, Kozlova YuV, Lysytsia O. [Pathomorphological aspects of explosive injury (comparative characteristics of injuries caused by OZM-72 and MON-50 anti-personnel fragmentation mines)]. Sudovo-medychna ekspertyza. 2017;(1):102-106. Ukrainian. DOI: https://doi.org/10.24061/2707-8728.1.2017.25
  4. Coccolini F, Panel TWE, Coimbra R, Ordonez C, Kluger Y, Vega F, Moore EE, Biffl W, Peitzman A, Horer T. Liver trauma: WSES 2020 guidelines. World J. Emerg. Surg. 2020;15:1–15.
  5. Shrestha A, Neupane HC, Tamrakar KK, Bhattarai A, Katwal G. Role of liver enzymes in patients with blunt abdominal trauma to diagnose liver injury. International. J Emerg Med. 2021;14(1):16-29. DOI: https://doi.org/10.1186/S12245-021-00332-1.
  6. Shrestha A, Bhattarai A, Bhandari R. Blunt abdomen trauma and biomarkers. Part of the Biomarkers in disease: methods, discoveries and applications. Springer, Cham. 2022;1:1-16. https://doi.org/10.1007/978-3-030-87302-8_5-1
  7. Law of Ukraine. [On the protection of animals from cruelty]. Information of the Verkhovna Rada of Ukraine. 2006;27:230. Ukrainian. Electronic resource: http://zakon1.rada.gov.ua/laws/show/3447-15.
  8. Kozlova YuV, Kosharnij AV, Korzachenko MA, Kytova IV. [Retrospective analysis and current state of experimental models of blast-induced trauma]. Ukrainskyi zhurnal medytsyny, biolohii ta sportu. 2020;5(28):66-71. Ukrainian. DOI: https://doi.org/ 10.26693/jmbs05.06.066
  9. Serov VV, Lapysh K, authors. Morfolojicheskaya diagnostica bolezney pecheni [Morphological diagnostics of liver diseases]. Moscow: Medytsyna; 1989.
  10. p. Russian.
  11. Cox JM, Kalns JE. Development and characterization of a rat model of nonpenetrating liver trauma. Comp Med. 2010;60(3):218-224.
  12. Sánchez-Valle V, Chávez-Tapia NC, Uribe M, Méndez-Sánchez N. Role of oxidative stress and molecular changes in liver fibrosis: a review. Curr Med Chem. 2012;19(28):4850-4860. DOI: 10.2174/092986712803341520.
  13. Luangmonkong T, Suriguga S, Mutsaers HAM, Groothuis GMM, Olinga P, Boersema M. Targeting oxidative stress for the treatment of liver fibrosis. Rev. Physiol. Biochem. Pharmacol. 2018;175:71-102. DOI: https://doi.org/10.1007/112_2018_10.
  14. Toshihiro W, Kurata S, Takuro S, Ichiro O, Yasuaki S, Ikeda T, Ureshino H, Ayuse T. Protective effect of nitric oxide on liver circulation from ischemia reperfusion injury. J Invest Surg. 2015;28(5):236-244. DOI: https://doi.org/10.3109/08941939.2015.1024357.

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Published

2022-10-07

How to Cite

Kozlov, . S., & Korzachenko , M. . (2022). Spatial-temporal distribution of expression of liver injury markers according to the results of immunohistochemical analysis after exposure to an air shock wave. Морфологія / Morphologia / Morfologìâ, 16(3), 56–60. Retrieved from https://morphology.dma.edu.ua/article/view/281904

Issue

Vol. 16 No. 3 (2022)

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