Features of morphological changes of the uterus after the death prescription

Authors

DOI:

https://doi.org/10.26641/1997-9665.2020.4.90-94

Keywords:

morphological changes, uterus, forensic medical examination

Abstract

Background. Assessing the postmortem interval (PMI) is one of the most problematic issues in judicial practice. Objective. To investigate the morphological changes of uterus tissues to determine the PMI-dependent features of postmortem changes development. Methods. A morphological analysis of 40 uterus tissue samples was performed; consisting of two groups: first group – samples from the corpses of women aged 23 to 70 years (n = 34), second group – comparison group – biopsy material from 6 women with uterine prolapse, uterine leiomyoma, from unaffected areas. Results. In the period from 24 to 48 hours after death, there were changes in the form of a slight change in the structure of cellular elements, changes in their color, the presence of light gaps between connective tissue and muscle fibers. In the period from 48 to 72 hours, the destruction of the border between the muscular and serous membranes, blurred contours and ruptures of cells, severe swelling of muscle fibers, wide gaps between muscle fibers, a significant decrease in fibroblastic cells were observed. In addition, there was no endothelium in a significant number of vessels, loss of clarity of fiber bundles, lack of nuclei in a significant number of myocytes. Desquamed endothelial cells and hemolyzed erythrocytes were seen in the openings of most vessels. Conclusion. Histological examination of uterine tissues showed the presence of specific changes during the studied time intervals after death, which can be used to introduce criteria to determine the time since death in practice.

References

  1. Šaňková M, Račanská M. Molekulární genetika a stanovení doby úmrtí [Molecular genetics and determination of time since death - short communication]. Soud Lek. 2016;61(3):28-9. Czech.
  2. Gelderman HT, Kruiver CA, Oostra RJ, Zeegers MP, Duijst WLJM. Estimation of the postmortem interval based on the human decomposition process. J Forensic Leg Med. 2019 Feb;61:122-7.
  3. Peyron, PA., Lehmann S, Delaby C, Baccino E, Hirtz C. Biochemical markers of time since death in cerebrospinal fluid: a first step towards “Forensomics.” Critical Reviews in Clinical Laboratory Sciences. 2019:1–37.
  4. Parmar AK, Menon SK. Estimation of postmortem interval through albumin in CSF by simple dye binding method. Sci Justice. 2015;55:388–93.
  5. Swain R, Kumar A, Sahoo J. Estimation of postmortem interval: a comparison between cerebrospinal fluid and vitreous humour chemistry. J Forensic Leg Med. 2015;36:144–8.
  6. Kumar S, Verma AK. Estimation of postmortem interval using the data of insulin level in the cadaver׳s blood. Data Brief. 2016 Mar 2;7:354-6.
  7. Li W, Chang Y, Han L, Liu X, Cai J, Zha L, Guo Y, Ding Y. Trimethylamine in postmortem tissues as a predictor of postmortem interval estimation using the GC method. Leg Med (Tokyo). 2018 Nov;35:80-5.
  8. Schwarcz HP, Agur K, Jantz LM. A new method for determination of postmortem interval: citrate content of bone. J Forensic Sci. 2010 Nov;55(6):1516-22.
  9. Pittner S, Ehrenfellner B, Zissler A, Racher V, Trutschnig W, Bathke AC, Sänger AM, Stoiber W, Steinbacher P, Monticelli FC. First application of a protein-based approach for time since death estimation. Int J Legal Med. 2017 Mar;131(2):479-83.
  10. Hostiuc S, Rusu MC, Mănoiu VS, Vrapciu AD, Negoi I, Popescu MV. Usefulness of ultrastructure studies for the estimation of the postmortem interval. A systematic review. Rom J Morphol Embryol. 2017;58(2):377-84.
  11. Pinheiro J. Decay Process of a Cadaver. In: Schmitt A., Cunha E., Pinheiro J. (eds) Forensic Anthropology and Medicine. Humana Press. 2006. p. 95.
  12. Mahalakshmi V, Gururaj N, Sathya R, Sabarinath TR, Sivapathasundharam B, Kalaiselvan S. Assessment of histological changes in antemortem gingival tissues fixed at various time intervals: A method of estimation of postmortem interval. J Forensic Dent Sci. 2016;8(2):114.
  13. Yadav AB, Angadi PV, Kale AD, Yadav SK. Histological assessment of cellular changes in postmortem gingival specimens for estimation of time since death. J Forensic Odontostomatol. 2015;33(1):19-26.
  14. Alibegović A, Balažic J, Petrovič D, Hribar G, Blagus R, Drobnič M. Viability of human articular chondrocytes harvested postmortem: changes with time and temperature of in vitro culture conditions. J Forensic Sci. 2014 Mar;59(2):522-8.
  15. Nagy C, Maheu M, Lopez JP, Vaillancourt K, Cruceanu C, Gross JA, Arnovitz M, Mechawar N, Turecki G. Effects of postmortem interval on biomolecule integrity in the brain. J Neuropathol Exp Neurol. 2015 May;74(5):459-69.

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Published

2021-09-25

How to Cite

Grygorian, E. K., & Myroshnychenko, M. S. (2021). Features of morphological changes of the uterus after the death prescription. Морфологія / Morphologia / Morfologìâ, 14(4), 90–94. https://doi.org/10.26641/1997-9665.2020.4.90-94

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