Morphological features of the epithelium of the mucous membrane of the bladder of rats after administration of the antigen

Authors

DOI:

https://doi.org/10.26641/1997-9665.2020.3.98-103

Keywords:

postnatal ontogenesis, bladder, antigenic stimulation, epithelium

Abstract

Background. Diseases of the urinary system and the bladder in particular (15-65% of the general pathology of the genitourinary system (SHS) is an urgent problem of medicine. The causes of these diseases are often associated with intrauterine infection. The effect of antigenic (AH) stimulation (immunomorphological influence) is poorly understood today, and aroused interest in this work. The aim of the study was to study the morphological features of the epithelium of the urinary bladder mucosa in rats during postnatal ontogenesis after intrauterine exposure to antigens. Methods. The study was carried out on 192 Wistar rats. Animals were divided into 3 groups: intact rats, control rats, which were injected with NaCl 0.9%, experimental rats, which were injected with staphylococcal toxoid intrafetally on the 18th day of intrauterine development. In each group, 64 animals were studied in 8 age periods: 1, 7, 14, 21, 30, 45, 60 and 90 days of life were taken into account (8 rats in each age cohort). The study was carried out using histological, morphometric and electron microscopic methods. The maintenance and manipulation of animals was carried out in accordance with the "General ethical principles of animal experiments." Results. When studying the urinary bladder mucosa of rats after exposure to the antigen, it was found that in the first 45 days there is a pronounced disorganization of the epithelium, retention of intracellular fluid, followed by partial restoration of structure by the end of the observation period. The thickness of the epithelium in experimental rats prevails over that in intact rats during most of the study period. It is important that by the end of the observation period (90 days) the epithelium in the experimental group is thinner than in the intact one.

References

  1. Burger M, Catto JW, Dalbagni G, Grossman HB, Herr H, Karakiewicz P, et al. [Epidemiology and risk factors of urothelial bladder cancer]. European urology. 2013; 63(2):234-241. English. doi: 10.1016/j.eururo. 2012.07.033.
  2. Voloshin NA, Svetlitsky AA, Chugin SV, Vasilchuk NG. [Intrauterine antigen administration is a risk factor for the formation of the organs of newborns] Pathologia. 2008; 5(4): 23-23. Ukrainian.
  3. Bondarenko NP, Zhezhera VM, Aksonova AV. [Fetal myocarditis – a precursor of undesirable consequences of intrauterine infection with parvovirus B19]. Pathologia. 2019; 16(1): 21–26. Ukrainian. DOI: 10.14739/2310-1237. 2019.1.166183
  4. Babintseva AG. [Pathogenetic aspects of the functional breakdown of the secular system in full-term newborn babies with pathology of the early neonatal period]. Neonatology, surgery and perinatal medicine. 2017; 3(25): 34–43. Ukrainian.
  5. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg: Council of Europe. 18 Mar 1986; 53 p.
  6. Kozhemyakin YuM, Khromov OS, Filonenko MA, Sayfetdinova GA, authors; Naukovo-praktychni rekomendatsiyi z utrymannya laboratornykh tvaryn ta roboty z nymy [Scientific and practical advice on the maintenance of laboratory animals and work with them]. Kyiv: Avitsenna; 2002. 265 р. Ukrainian.
  7. Merkulov GA, authors; Kurs patohistologicheskoy tehniki [Course of pathohistological techniques] Leningrad: Meditsina; 1969. 87 р. Russian.
  8. Avtandilov GG, author. Osnovy kolichestvennoy patologicheskoy anatomii [Fundamentals of quantitative pathological anatomy]. Moscow: Meditsina; 2002. 308 р. Russian.
  9. Lapach SN, Chubenko AV, Babych PN. [Statistical methods in medical-biological studies using Exel]. Kiev: Morion; 2000. 320 р. Russian

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How to Cite

Khitrik, A. J. (2020). Morphological features of the epithelium of the mucous membrane of the bladder of rats after administration of the antigen. Морфологія / Morphologia / Morfologìâ, 14(3), 98–103. https://doi.org/10.26641/1997-9665.2020.3.98-103

Issue

Vol. 14 No. 3 (2020)

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Статті

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