Morphological features of testicular and epididymal spermatozoa obtained from azoospermic men.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2020.1.58-61

Keywords:

sperm, infertility, obstructive azoospermia, non-obstructive azoospermia, morphological characteristics

Abstract

Background. Studying of morphological features of spermatozoa obtained from men with azoospermia is due to the high rate of infertile marriages with a male factor. It is extremely important to estimate spermatozoa morphological characteristics, which can be a predictor of their fertilizing ability and therefore improve assisted reproductive technology (ART) results. Objective. The aim of the study was to assess the morphological characteristics of spermatozoa obtained during surgical inventions in patients with azoospermia. Methods. Evaluation of sperm morphology was performed using light microscopy. Statistic methods was used for data processing. Results. Morphological characteristics of spermatozoa obtained from patients with non-obstructive azoospermia were characterized by a higher rate of mixed pathology (head, neck and tail) then morphology of spermatozoa from men with obstructive azoospermia and normozoospermia (25 9 ± 1.38% vs 22.8 ± 2.44% and 34.6 ± 4.21%, respectively). Conclusion. The obtained morphology data of spermatozoa from men with obstructive and non-obstructive azoospermia will allow us to introduce an algorithm for assessing sperm quality for patients undergoing ART.

References

  1. Gorpinchenko II, Romanyuk MG. [Male infertility: etiology, pathogenesis, diagnosis and current treatments]. Men's health. 2016;1(56):8-17. Ukrainian.
  2. Nurimanov KR. [Clinical and laboratory characteristics of male infertility in azoospermia]. Men's Health. 2004;4(11):139-140. Ukrainian.
  3. Petrushko MP. [The current state of the problem of cryopreservation of human reproductive cells and embryos]. Bulletin of the National Academy of Sciences of Ukraine. 2017;(7):44-52. Ukrainian. doi: https://doi.org/10.15407/visn2017.07.044
  4. Desai N, Gill P, Tadros NN. Azoospermia and embryo morphokinetics: testicular sperm-derived embryos exhibit delays in early cell cycle events and increased arrest prior to compaction. J Assist Reprod Genet. 2018;35(7):1339–48. doi: 10.1007/s10815-018- 1183-8
  5. Petrushko MP, Pinyayev VI, Podufaliy VV. [Pregnancy after embryo transfer obtained by oocyte fertilization, without of Zona pellucida, with cryopreserved epididymal sperm (case report)]. Problems of reproduction. 2013;2:63 5. Ukrainian.
  6. World Health Organization. [Who Laboratory Manual for the Examination of Human Semen and Sperm‒Cervical Mucus Interact]. 2010. UP: 287 p.
  7. Grischenko VI, Petrushko MP, Pinyayev VI. [Effectiveness of IVF and ET programs depending on the quality and number of embryos transferred]. Problems of reproduction. 2000;6(1):44-7. Ukrainian.
  8. Yurchuk, T., Petrushko, M. & Fuller, B. [Science of cryopreservation in reproductive medicine - Embryos and oocytes as exemplars]. Early Hum. Dev. 2018;14:S0378-3782(18)30559-60. Ukrainian. doi: 10.1016/j.earlhumdev.2018.08.016
  9. Chang V, Heutte L, Petitjean C. Automatic classification of human sperm head morphology. Comput Biol Med. 2017;84:205–16. doi:10.1016/j.compbiomed.2017.03.029
  10. Shaker F, Monadjemi SA, Alirezaie J. A dictionary learning approach for human sperm heads classification. Comput Biol Med. 2017;91:181–90. doi:10.1016/j.compbiomed.2017.10.009
  11. Cao X, Cui Y, Zhang X. The correlation of sperm morphology with unexplained recurrent spontaneous abortion: A systematic review and meta-analysis. Oncotarget. 2017;8:55646-56. DOI: 10.18632 / oncotarget.17233
  12. Setti AS, Braga DP, Vingris L. Sperm morphological abnormalities visualised at high magnification predict embryonic development, from fertilisation to the blastocyst stage, in couples undergoing ICSI. J Assist Reprod Genet. 2014;31(11):1533–9. doi:10.1007/s10815-014-0326-9
  13. Petrushko MP, Yurchuk TA, Pinyayev VI. [Penetration test with Zona pellucida as a predictor of fertilizing ability of native and cryopreserved human sperm]. Ukrainian Journal of Medicine, Biology and Sports. 2017;1(1):189-92. Ukrainian.
  14. Petrushko МP, Pavlovich EV, Pinyaev VІ. [Apoptosis and processes of DNA fragmentation in native and cryopreserved human sperm cells at normo- and pathospermia]. Tsitol Genet. 2017;51(4):278–81. Ukrainian. doi: 10.3103/S0095452717040065
  15. Petrushko M.P., Pavlovich O.V., Pinyaev V.I. [Processes of DNA fragmentation and peroxidation of lipid in spermatozoa in humans under the norm of pathosperm]. Newsletter of Lviv University. Seriya Biologiya. 2016;74(97):97-103. Ukrainian.
  16. Ray PF, Toure A, Metzler-Guillemain C. Genetic abnormalities leading to qualitative defects of sperm morphology or function. Clin Genet. 2017;91(2):217–32. doi:10.1111/cge.12905

How to Cite

Panasovskiy, M. L. (2020). Morphological features of testicular and epididymal spermatozoa obtained from azoospermic men. Морфологія / Morphologia / Morfologìâ, 14(1), 58–61. https://doi.org/10.26641/1997-9665.2020.1.58-61

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