Comparative immunohistochemical study of neoangiogenesis in polyps and adenocarcinoma of the distal colon.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2019.4.43-49

Keywords:

polyps, colorectal cancer, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-1, Vascular Endothelial Growth Factor Receptor-2, CD34 Antigen

Abstract

Background. Neoangiogenesis provides trophic support for neoplastic cells, thus being an integral part of carcinogenesis. However, the features of neoangiogenesis in the most common distal colonic polyps and carcinoma are not completely studied yet. Objective. To compare VEGF-A, VEGFR-1, VEGFR-2, CD34 expression levels in distal colonic polyps and colorectal adenocarcinoma. Methods. Pathomorphological and immunohistochemical studies of biopsies of distal colonic polyps from 30 patients and surgical material of colorectal adenocarcinoma from 40 patients were carried out. Results. Distal colonic polyps are characterized by medium and low levels of VEGF-A, VEGFR-2 and CD34 expression. Wherein, VEGF-A and VEGFR-2 expression levels in adenomas are significantly higher than the same indicators in hyperplastic polyps: 25,51 (15,25 ; 30,19) vs. 12,82 (10,85 ; 15,00) CUOD and 47,18 (42,02 ; 62,13) vs. 37,08 (30,19 ; 45,12) CUOD, accordingly, p < 0,05. Comparative analysis of the data, obtained for distal colonic polyps and colorectal adenocarcinoma of the I stage shows significant difference between these groups by indicators of VEGF-A expression level and microvascular density, evaluated by CD34 expression. So, VEGF-A expression level in carcinoma of the I stage significantly higher the same indicator in adenomas and hyperplastic polyps: 37,80 (30,22; 56,89) vs. 25,51 (15,25 ; 30,19) CUOD and 37,80 (30,22; 56,89) vs. 12,82 (10,85 ; 15,00) CUOD, accordingly, p < 0,05. Microvascular density is significantly higher in non-invasive cancer in comparison to the polyps: 91,50 (51,00; 111,00) vs. 47,00 (33,00 ; 54,00) (adenomas) and 91,50 (51,00; 111,00) vs. 46,00 (30,00 ; 55,00) (hyperplastic polyps), p < 0,05. Conclusion. Adenomas in comparison to hyperplastic polyps are differ by significantly higher VEGF-A and VEGFR-2 expression levels, while colorectal adenocarcinoma of the I stage in comparison to distal colonic polyps differs by significantly higher VEGF-A and CD34 expression expression levels. Activation of neoangiogenesis occurs at the stage of transformation of distal colon polyps into non-invasive cancer.

References

  1. Asadzade-Aghdaei H, Pezeshkian Z, Atitappeh M, Nazemalhosseini-Mojarad E, Zali M. The Role of Angiogenesis in Colorectal Polyps and Cancer: a Review. Medical Laboratory Journal. 2018;12(4):1-6.
  2. Salmo E, Haboubi N. Adenoma and Malignant Colorectal Polyp: Pathological Considerations and Clinical Applications. European Medical Journal. 2018;7(1):92-102.
  3. Cassese G, Amendola A, Maione F, Giglio MC, Pagano G, Milone M, Aprea G, Luglio G, Palma GD. Serrated Lesions of the Colon-Rectum: A Focus on New Diagnostic Tools and Current Management. Gastroenterology Research and Practice. 2019;19:1-14.
  4. Yamane L, Scapulatempo-Neto C, Reis RM, Guimarães DP. Serrated pathway in colorectal carcinogenesis. World Journal of Gastroenterology. 2014;20(10):2634-40.
  5. Bozman FT, Carneiro F, Hruban RH, Stanley RN. WHO classification of tumours. Pathology and genetics. Tumours of the digestive system. Berlin: Springer-Verlag; 2010. 314 p.
  6. Liu H, Wu J, Liu XC, Wei N, Liu KL, Ma YH, Chang H, Zhou Q. Correlation between microvascular characteristics and the expression of MVD, IGF-1 and STAT3 in the development of colonic polyps carcinogenesis. Experimental and Therapeutic Medicine. 2017;13(1):49-54.
  7. Tiwari AK, Crawford SE, Radosevich A, Wali RK, Stypula Y, Kunte DP, Mutyal N, Ruderman S, Gomes A, Cornwell ML, De La Cruz M, Brasky J, Gibson TP, Backman V, Roy HK. Neo-angiogenesis and the premalignant micro-circulatory augmentation of early colon carcinogenesis. Cancer Letters. 2011;306(2):205-13.
  8. Sobin LH, Gospodarowicz MK, Wittekind C. International Union Against Cancer (UICC): TNM Classification of Malignant Tumours. New York: Wiley-Blackwell; 2009. 50 p.
  9. Bosari S, Lee AK, DeLellis RA, Wiley BD, Heatley GJ, Silverman ML. Microvessel quantitation and prognosis in invasive breast carcinoma. Human Pathology. 1992;23:755-61.
  10. Shyskin MA. [Immunohistochemical study of VEGF-A, VEGFR-1, VEGFR-2 and CD34 expression in сolorectal adenocarcinoma progression]. Pathologia. 2019;2(46):148-54. Russian.
  11. Ruffolo C, Toffolatti L, Canal F, Kotsafti A, Pagura G, Pozza A, Campo Dell'Orto M, Ferrara F, Massani M, Dei Tos AP, Castoro C, Bassi N, Scarpa M. Colorectal polypoid lesions and expression of vascular endothelial growth factor in a consecutive series of endoscopic and surgical patients. Tumour Biology. 2017;39(3):1-9.
  12. Tjalma JJ, Garcia-Allende PB, Hartmans E, Terwisscha van Scheltinga AG, Boersma-van Ek W, Glatz J, Koch M, van Herwaarden YJ, Bisseling TM, Nagtegaal ID, Timmer-Bosscha H, Koornstra JJ, Karrenbeld A, Kleibeuker JH, van Dam GM, Ntziachristos V, Nagengast WB. Molecular Fluorescence Endoscopy Targeting Vascular Endothelial Growth Factor A for Improved Colorectal Polyp Detection. Journal of Nuclear Medicine. 2016;57(3):480-5.
  13. Qasim BJ, Ali HH, Hussein AG. Immunohistochemical expression of PCNA and CD34 in colorectal adenomas and carcinomas using specified automated cellular image analysis system: A clinicopathologic study. Saudi Journal of Gastroenterology. 2012;18(4):268-76.
  14. Behbehani A, Ranjbari N, Rahim F, Jazayeri N. Evaluating the expression of CD34 marker in colorectal adenocarcinoma and its relationship with clinicopathologic factors. Asian Journal of Cell Biology. 2015;10(3):80-6.
  15. Heljasvaara R, Aikio M, Ruotsalainen H, Pihlajaniemi T. Collagen XVIII in tissue homeostasis and 7 dysregulation — Lessons learned from 8Q1 model organisms and human patients. Matrix Biology. 2017;57:55-75.
  16. Morin PJ, Kinzler KW, Sparks AB. β-Catenin Mutations: Insights into the APC Pathway and the Power of Genetics. Cancer Research. 2016; 76(19):5587-9.

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Shyshkin, M. A., & Khrystenko, T. A. (2019). Comparative immunohistochemical study of neoangiogenesis in polyps and adenocarcinoma of the distal colon. Морфологія / Morphologia / Morfologìâ, 13(4), 43–49. https://doi.org/10.26641/1997-9665.2019.4.43-49

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Vol. 13 No. 4 (2019)

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