Morphological characteristics of the visual cortex in experimental diabetes mellitus.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2019.3.170-175

Keywords:

optic cortex, neuron, , streptozotocin diabetes mellitus

Abstract

Background. The medical and social problem of diabetes mellitus (DM) is due to the significant prevalence, early disability and death of patients due to specific complications, one of which is diabetic encephalopathy. According to scientific literature, the latter is diagnosed in 80.7% of patients with type 1 diabetes. Objective of our work was to establish histo-ultrastructural changes in the visual cortex on the 56th day of the development of streptozotocinin-induced diabetes mellitus (SSD). Methods. For the study, 10 sexually mature white male rats (body weight 160-180 g) were used, which were equally divided into 2 groups: the first was a research one with SDS (single intraperitoneal administration of streptozotocin (SIGMA Chemical USA) at a rate of 6 mg / 100 g weight body), the second - control. For morphometric studies, the NIH USA “Image J” software and the statistical package Stat.Soft.Inc were used; Tulsa, OK, USA; Statistica 10. Results. In animals with SJS, in contrast to the control group of rats, the number of vacuolated and pycnomorphic neurons increases against the background of a decrease in normochromic neurons by 0.01 mm2 of the visual cortex. At the same time, the glial index increases by 1.8 times, and the number of capillaries by 0.01 mm2 decreases by 2.2 times. At the ultrastructural level, the most pronounced changes are experienced by pycnomorphic neurons, which are localized in the inner pyramidal layer. In the outer pyramidal and inner granular layers of the visual cortex, neurons with partial necrosis are found. Such changes in the visual cortex occur against the background of the development of diabetic microangiopathy. Thus, on day 56 of the course of SJS in the visual cortex of rats, diabetic encephalopathy is diagnosed, which is morphologically manifested: an increase in the numerical density of pycnomorphic and vacuolated neurons due to a decrease in normochromic neurons; phenomena of satellite disease and neuronophagy; axonopathy in tangential bundles of nerve fibers of the neuropil. Conclusion. Such changes occur against the background of the development of diabetic microangiopathy, which is characterized by: erythrocyte sludge, platelet adhesion and microthrombi in the lumen of microvessels; an increase in the capillary wall area with a decrease in the area of their lumen; thickening of the basement membrane; pericapillary edema of the legs of astrocytes.

References

  1. Sleep GO. [Current approaches to the treatment of diabetic retinopathy]. Advances in biology and medicine. 2017;30(2):75-80. Ukrainian.
  2. Tumansky VA, Avramenko UN. Some aspects of morphogenesis of diabetic encephalopathy. Zaporozhye Medical Journal.2013;79(4):39–42. Russian.
  3. Essin WG, Khairullin IX, Ziangirova ST, Isaeva UN, Essin OR. [Diabetic encephalopathy: pathogenesis, clinical manifestations, approaches to therapy]. Clinician. 2012;3-4:10-16. Russian.
  4. Zhurakivska OY, Popovich YI, Oliynyk YI, Miskiv VA, Zhurakivsky VM, Tymoschuk OJ. [Features of morphofunctional restructuring of the hypothalamic-neurophipophyseal system of immature rats in the early stages of experimental diabetes]. Bulletin of problems of biology and medicine. 2018;145(3):288-294. Ukrainian.
  5. Brismar T, Hyllienmark , Ekberg K, Johansson BL.[ Loss of temporal lobe betapower in young adults with type 1 diabetesmellitus]. Neuroreport. 2002;13(18):2469–73.
  6. Sima AA, Zhang W, Muzik O. [Sequentialabnormalitiesintype 1 diabeticencephalopathyandtheeffectsof C-peptide]. RevDiabetStud 2009;6(3):211–22.
  7. Sima AA. [Encephalopathies: theemergingdiabeticcomplications]. Acta Diabetol.2010;47(4):279–93.
  8. Malone JI, Hanna S, Saporta S. etal. [Hyperglycemia not hypoglycemia alters neuronal dendrites and impairsspatialmemory]. PediatrDiabetes. 2008;9(6):531–9.
  9. Roustit M, Loader J, Deusenbery C, Baltzis D, Veves A. Endothelial Dysfunction as a Link Between Cardiovascular Risk Factorsand Peripheral Neuropathyin Diabetes. J ClinEndocrinolMetab. 2016; 101(9): 34018.
  10. Yang X, Huo F, Liu B, Liu J, Chen T, Li J, Zhu Z, Lv B. [CrocinInhibits Oxidative Stress and Pro-inflammatory Response of Microglial Cells Associated with Diabetic RetinopathyThroughtheActivationof PI3K/AktSignalingPathway]. J MolNeurosci. 2017; 61(4): 581-589. doi: 10.1007/s12031-017-0899-8.

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How to Cite

Shchur, M. B., & Zhurakivska, O. Y. (2019). Morphological characteristics of the visual cortex in experimental diabetes mellitus. Морфологія / Morphologia / Morfologìâ, 13(3), 170–175. https://doi.org/10.26641/1997-9665.2019.3.170-175

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Vol. 13 No. 3 (2019)

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