Comparative morphology of regenerative inflammatory processes in the skin using peptide activators and preparations based on hyaluronic acid.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2019.3.126-132

Keywords:

peptide activator, preparation based on hyaluronic acid, collagen, angiogenesis, collagenogenesis, fibroblasts

Abstract

Background. The rapid development of innovative technologies, particularly in the medicine, requires a continuous update of the arsenal of knowledge and understanding of processes in connection with the introduction of new techniques. Angiogenesis is an extremely important process for the formation of regenerate, since it is the growth of new microvessels that accompanies the migration of cells of the fibroblast row to the site of alteration and provides their metabolic needs. If angiogenesis is stimulated in insufficient amounts, migration of fibroblasts, as well as the processes of regenerationslows down or even stops. The objective of the study was to explore and compare the morphological changes that occur in the dermis at different chronological stages when using peptide activators and preparations based on hyaluronic acid. Methods. The research was conducted on the basis of Danylo Halytsky Lviv National Medical University. For the experiment, 30 adult white rats, 150-180 g were used, which were divided into 3 groups of 10 rats, one of which was administered a peptide activator drug, the other 10 rats were given a hyaluronic acid-based drug, another 10 - control group, which was injected with an isotonic solution of 0.9% NaCl. The rats were removed from the experiment on days 14, 28, and 42 of the study by overdose of diethyl ether, followed by resection of the skin flap from the injection site. Subsequently, hematoxylin-eosin staining, histochemical technique by Malory and immunohistochemical staining using CD34 antibody was performed. Results. It was found that on 42 day of the experiment, there was a slight setback in both cases after a rapid increase in connective tissue volume, in particular collagen at the injection sites. However, angiogenesis stimulated by peptide activators was more active and persistent than in rats administered a hyaluronic acid-based preparation. Conclusion. In the future, it is planned to continue the study of morphological changes in the skin on day 56 in order to confirm the results obtained in the previous stages.

References

  1. Diskovskiy І. С. [Morphological specialties and processes of reparation in full experiment in experiment. [abstract]. Ivano-Frankivsk: The sovereign first and foremost mortgage "Ivano-Frankivsk national medical university"]. 2016; 19 sec. Ukrainian
  2. Kochergin NG, Tkachenko GA. [Age and skin]. Clinical Gerontology. 2007;12:60-62. Russian
  3. Margolin AA. [Skin photoaging prevention and treatment]. Cosmetics and Medicine. 2001;2:44-53. Russian
  4. Vissarionov VA, Burylina OM, Ivontiev GA. [Actual issues of plastic, aesthetic surgery and dermatocosmetology]. Moskva; 2007. 178 p. Russian
  5. Tamrazova AB. [Prevention of skin aging. Experimental and clinical dermatocosmetology]. 2007;1:56-60/ Russian
  6. Baygildina AA. [Features of the metabolism and structural and functional state of the endothelium in hemorrhagic fever with renal syndrome: abstract. dis. ... doctor. honey. Sciences]. Novosibirsk; 2014. 34 p. Russian
  7. Mayborodin IV, Shevela AI, Matveeva VA, Drovosekov MN, Barannik MI, Kuznetsova IV. [Angiogenesis in granulation tissue after implantation of a polyhydroxyalkanoate with mesenchymal stem cells]. Surgery News. 2013;21(2):29–36. Russian
  8. Hoeben A., Landuyt B., Highley M. S. Vascular endothelial growth factor and angiogenesis. PharmacolRev. 2004;56(4):549–580.
  9. Lyons S. M., Fay M. M., Akiyama Y. RNA biology of angiogenin: Current state and perspectives. RNA Biol. 2017;14(2):171–178.
  10. Wang H., Shi L., Qin J., Yousefi S., Li Y., Wang R. K. Multimodal optical imaging can reveal changes in microcirculation and tissue oxygena- tion during skin wound healing. LasersSurg. Med. 2014;46(6):470–478.

Downloads

How to Cite

Turkevich, M. O., Turkevich, O. O., & Pospishil, Y. O. (2019). Comparative morphology of regenerative inflammatory processes in the skin using peptide activators and preparations based on hyaluronic acid. Морфологія / Morphologia / Morfologìâ, 13(3), 126–132. https://doi.org/10.26641/1997-9665.2019.3.126-132

Issue

Vol. 13 No. 3 (2019)

Section

Статті

License

Copyright (c) 2019 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.