Gisto-ultrastructural changes of temporal muscle in the early stages of streptozotocin diabetes mellitus.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2019.3.60-65

Keywords:

temporal muscle, muscle fibers, streptozotocin diabetes mellitus

Abstract

Background. Skeletal muscles are responsible for glucose utilization under the influence of insulin, so they play an important role in glucose metabolism. They are the prime target for insulin action in regulating of blood glucose levels by activating the insulin-sensitive glucose transporter (GLUT-4) on the plasma membrane of the cell. Objective. Therefore, the aim of our work was to establish histological and ultrastructural changes of the temporal muscle oftheratonthe 14th and 28th day of the development of streptozotocin DM (SDM). Methods. 16 adult white male rats (bodyweight 160-200 g) were used for the study. All animals were divided into 2 groups: the first - experimental (10 animals) with SDM (single intraperitoneal injection of streptozotocinat the rate of 6 mg/100 g of bodyweight), the second control (6 animals). Material sampling was carried out on the 14th and 28th days from the beginning of the SDM simulation. Results. On the 14-28th day of the SDM development, there is a significant decrease of the area of muscle fibers in 1.5-1.4 times due to the decrease of the amount of glycogen, which is confirmed by histological and ultrastructural studies. There are initial signs of diabetic microangiopathy, which morphologically manifests itself as a spasm of the afferent vessels, which is confirmed by: a decrease in the area of the lumen of arterioles and an increase on their Vogenvort index; reduced number of capillaries on 0.1 mm2by 1.2 times on the 28th day of the experiment and by an increased area of their wall and Vogenvort index by 1.4 times and 1.3 times, respectively; hemorheological disorders in vessels (erythrocyte clots, adhesion of erythrocytes and platelets, microclasmatosis). Such changes in the vessels of hemomicrocirculatory blood flow lead to muscle hypoxia, that’s why at the submicroscopic level their mitochondria have the most pronounced changes, while the myofibrils retain their ultrastructure. Conclusion. Thus, in early stages of SDM development (14-28 days) on a background of hyperglycemia in temporal muscle we observe: a decrease in the area of muscle fibers due to the reduction in the amount of glycogen in them. In the vessels of the hemomicrocirculatory blood flow, the initial signs of diabetic microangiopathy are manifested, which is characterized by a spasm of afferent vessels, a decrease in the number of capillaries on 0.1 mm2 and an increase in the area of their wall and Vogenvort index, hemorheological disorders in the exchange vessels.

References

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Koshkin, O. Y., & Zhurakivska, O. Y. (2019). Gisto-ultrastructural changes of temporal muscle in the early stages of streptozotocin diabetes mellitus. Морфологія / Morphologia / Morfologìâ, 13(3), 60–65. https://doi.org/10.26641/1997-9665.2019.3.60-65

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Vol. 13 No. 3 (2019)

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