Immunomorphological features of cervical dysplasia depending on the progression of papillomavirus infection in women with infertility.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2019.3.48-53

Keywords:

cervical dysplasia, infection of papillomavirus, infertility

Abstract

Background. With the development and improvement of modern methods of immunohistochemical examination, new possibilities for studying the molecular-biological features of cervical dysplasia (CD/CIN) emerged, opening new stages of its morphogenesis. Taking into account the demographic situation in Ukraine, and the fact that incidence rate of squamous cell intraepithelial cervical lesions and infertility have a tendency to increase, it becomes apparent the need for further investigation of CIN associated with the (HPV) infection. Objective. The purpose of the study was to determine the immunohistochemical features of cervical dysplasia in women with infertility, depending on the progression of papillomavirus infection. Methods. Specific quantitative diagnostics of real-time polymerase-chain reaction with hybridization-fluorescence detection was used to detect papillomavirus infection. Immunohistochemical studies used primary monoclonal antibodies to Ki-67, p63, VEGF, p16ink4a, estrogen (ER) and progesterone receptors (PR), E-cadherin. The results obtained during the study were subjected to statistical processing by methods of variational statistics. Results. Immunomorphological features of cervical dysplasia depending on the progression of papillomavirus infection have been established in women with infertility, and the reliable influence of HPV WRC loading on the level of expression of molecular-biological markers depending on the severity of cervical dysplasia has been demonstrated. Conclusion.Therefore, the use of Ki-67, p63, pl6ink4a, VEGF, E-cadherin markers is the most optimal for the implementation of the diagnostic process and for determining the course of CIN, since the change in their expression most often corresponded to the severity of CIN.

References

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How to Cite

Kindrativ, E. O. (2019). Immunomorphological features of cervical dysplasia depending on the progression of papillomavirus infection in women with infertility. Морфологія / Morphologia / Morfologìâ, 13(3), 48–53. https://doi.org/10.26641/1997-9665.2019.3.48-53

Issue

Vol. 13 No. 3 (2019)

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Статті

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