Morphological rearrangements of the rat’s adrenal cortex under conditions of prolonged exposure to heavy metal salts and non-hormonal correction.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2019.3.26-31

Keywords:

adrenal glands, heavy metals, adaptation syndrome, vitamin E

Abstract

Background. Heavy metal salts adversely affect the health of the population and cause the development of pathology of individual organs and systems of the body. The adrenal glands occupy one of the key places in the regulation and maintenance of the basic functions of the body. Objective. The study of morphological adaptive rearrangements of the structural components of the adrenal cortex of adult mature males under conditions of prolonged exposure to heavy metal salts and non-hormonal correction by vitamin E remains a relevant and under-studied topic. Methods. The experiment was conducted on 24 white mature male rats weighing 200-250 g at the age of 5-6 months, which after a long (90-day) influence of the combination of salts of heavy metals: zinc (ZnSO4 · 7H2O) - 5 mg / l, copper (CuSO4 5H2O) - 1 mg / l, iron (FeSO4) - 10 mg / l, manganese (MnSO4 · 5H2O) - 0.1 mg / l, lead (Pb (NO3) 2) - 0.1 mg / l and chromium (K2Cr2O7) - 0.1 mg / l for 30 days consumed or dinary drinking water with vitamin E correction drug (9.1 mg / kg 10% oil solution). The conventional histological examination methods were applied. Results. The antioxidant L-tocopherol, upon admission to the body of a combination of salts of heavy metals, revealed a reliable stress-protective effect on all the structural components of the adrenal gland: the state of the vascular wall, the nuclear and secretory apparatus spongiocytes of all zones of the cortex. Conclusion. L-tocopherol helps to reduce the toxic effects of heavy metal salts on the adrenal cortex, exerts a membrane-protective and stress-protective effect, attenuates the manifestations of the stage of exhaustion of the general adaptation syndrome, partially restores the hormonal activity of adrenocorticocytes. However, the 30-day period of correction of morphological alterations caused by the entry into the body of heavy metal salts is insufficient for the complete restoration of the state of the vascular wall, the rheological properties of blood and the secretory activity of cells of the adrenal cortex.

References

  1. Wei X, Gao B, Wang P, Shu T, Xiong Q, Chen F.Pollution characteristics and health risk assessment of heavy metals in streetdusts from different functional areas in Beijing. Ecotoxicol. Environ. Saf.2015;112:186-92. doi: 10.1016/j.ecoenv.2014.11.005 https://doi.org/10.1016/j.ecoenv.2014.11.005.
  2. Rohr JR, Farag AM, Cadotte MW, Clements WH, Smith JR, Ulrich CP, etal. Transforming Ecosystems: When, Where, and How to Restore Contaminated Sites. Integr Environ Assess Manag. 2016;12(2):273–283. doi:10.1002/ieam.1668https://doi.org/10.1002/ieam.1668
  3. Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary Toxicology. 2014;7(2):60-72. doi:10.2478/intox-2014-0009https://doi.org/10.2478/intox-2014-0009
  4. Vukosav P, Mlakar M, Cukrov N, Kwokal Z, Pižeta I,Pavlus N, etal. Heavy metal contents in water, sediment and fshin a karstaquatic ecosystem of the Plitvice Lakes National Park (Croatia). Environ. Sci. Pollut. Res.2014;21(5):3826-39. doi:10.1007/s11356-013-2377-3https://doi.org/10.1007/s11356-013-2377
  5. Mosa KA, Saadoun I, Kumar K, Helmy M, Dhankher OP. Potential Biotechnological Strategies for the Clean up of Heavy Metals and Metalloids. Front Plant Sci. 2016;7:303. doi:10.3389/fpls.2016.00303https://doi.org/10.3389/fpls.2016.0030.
  6. Hryntsova NB. [Morphological rearrangements of structural components of the intermediate part of the pituitary glando fmature female rats under the influence of salts of heavy metals]. In: [15–th International Scientific European Conference on Innovations in Technical and Natural Sciences; 2017 July 20; Vienna, Austria]. East West, 2017. р. 3-8. Ukrainian.
  7. Bhattacharya PT, Misra SR, Hussain M. Nutritional Aspects of Essential Trace Elements in Oral Health and Disease: An Extensive Review. Scientifca (Cairo). 2016;2016:5464373. doi:10.1155/2016/5464373https://doi.org/10.1155/2016/5464373.
  8. Järup L. Hazards of heavy metal contamination. BrMedBull. 2003;68(1):167–182. https://doi.org/10.1093/bmb/ldg032
  9. Hinson, JP., Raven, PV. Effects of endocrine-disrupting chemicals on adrenal function. Best Pract. Res. Clin. Endocrinol. Metab. 2006:111–120.
  10. Sharma B, Singh S, Siddiqi NJ. Biomedical implications of heavy metals induced imbalances in redox systems. Biomed Res Int.2014:640754. doi:10.1155/2014/640754https://doi.org/10.1155/2014/640754
  11. Poljsak B, Šuput D, Milisav I. Achieving the Balance between ROS and Antioxidants: When to Use the Synthetic Antioxidants. OxidMedCellLongev.2013;2013:956792. doi:10.1155/2013/956792https://doi.org/10.1155/2013/956792
  12. Al-Attar AM. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J BiolSci. 2011Jan;18(1):63-72. doi:10.1016/j.sjbs.2010.10.004.
  13. Hryntsova NB, Romaniuk AM, Boumister VI, Kiptenko LI, Pernakov MS. [Morphofunctional remodeling of the adrenal cortex of pubescent rats under conditions of long-term exposure to heavy metal salts]. Bulletin of Vinnitsa National University. 2019;23(1):54-58. Ukrainian.
  14. Shkorbatov YuG. [Structural and electrokinetic properties of nuclei of buccal epithelium cells due to the action of physicochemical factors and changes in the functional state of the body]. Abstract of dissertation for a scientific degree doctor of Biological Sciences. Specialty 03. 00. 11 - cytology, cellbiology, histology. 2005. Kyiv. р. 27. Ukrainian

Downloads

How to Cite

Hryntsova, N. B., Romanіyk A. M., & Bumeister, V. I. (2019). Morphological rearrangements of the rat’s adrenal cortex under conditions of prolonged exposure to heavy metal salts and non-hormonal correction. Морфологія / Morphologia / Morfologìâ, 13(3), 26–31. https://doi.org/10.26641/1997-9665.2019.3.26-31

Issue

Vol. 13 No. 3 (2019)

Section

Статті

License

Copyright (c) 2019 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.