Electron microscopic studies of rat periodontal tissues are normal.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2019.2.76-80

Keywords:

periodontal, electron microscopy, intact rats

Abstract

Background. The use of an electron microscopic study to study the structural organization of cells can be used as a standard with further histological studies and the characterization of morphological changes in pathologically altered periodontal tissues. The assessment of normal unchanged tissue provides important data for the analysis of pathological disorders or the activation of regenerative processes in periodontal structures. Objective. The purpose of the work was to investigate the ultrastructural organization of soft tissues of the periodontal complex of the rat in norm. Methods. In the experiment, 12 non-breeding white male rats, age 3.5 months, body weight 130-160 g were used. For conducting an electron microscopic examination, pieces of soft periodontal tissues were cut out within the gums papilla from different parts of the upper and lower jaws of the rat. Small pieces of tissue were fixed in 2.5% glutaraldehyde solution, in 1% solution of tetraoxide osmium on phosphate buffer pH 7.2-7.4., dehydrated in alcohols and propylene oxide and poured into a mixture of epoxy resins with araldit. Ultra-thin slices, made on ultramicrotome УМПТ3М studied in an electron microscope ПЕМ-100-01. Results. It was found that cells of the basal and spinous layers form a germic area of the epithelium. The granular layer is a small width, the cells are osmiumophilic, with uneven contours of karyoles of the nucleus. The horn is formed by degenerative altered epithelial cells. he periodontium consists of collagen fibers, layers of loose connective tissue, as of fibroblasts, basophils, lymphocytes and plasmocytes. In the lumen of the hemocapillary, red blood cells are observed, the wall forms endothelial cells and basement membrane. Conclusion. Thus, conducted electron microscopic studies of soft tissues of periodontal rats in norm established specific features of the structure of the gums, periodontal and hemocapillars of the gum mucosa.

References

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How to Cite

Fik, V. B., Chelpanova, I. V., Paltоv E. V., & Kovalyshyn, O. A. (2019). Electron microscopic studies of rat periodontal tissues are normal. Морфологія / Morphologia / Morfologìâ, 13(2), 76–80. https://doi.org/10.26641/1997-9665.2019.2.76-80

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Vol. 13 No. 2 (2019)

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