Morphofunctional peculiarities of the rat heart in experimental myocardial infarction.

Authors

  • B. O. Nadraga Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • A. M. Yashchenko Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  • A. D. Lutsyk Danylo Halytsky Lviv National Medical University, Lviv, Ukraine https://orcid.org/0000-0001-6819-804X

DOI:

https://doi.org/10.26641/1997-9665.2019.1.32-37

Keywords:

experimental adrenalin-induced myocardial infarction, rats, morpho-histochemical characteristics, marker enzymes

Abstract

Background. For better understanding of the nature of myocardial infarction and processes occurring in the myocardium during this acute process in order to develop more effective methods for its prevention and treatment, further experimental studies are needed. Objective. The aim of present investigation was to study morphofunctional and histochemical characteristics of the rat heart muscle under the conditions of experimental myocardial infarction. Methods. Experiments were carried out on 20 mature male Wistar rats weighing 180-220 g (5 control and 15 experimental animals). Acute myocardial infarction was induced by subcutaneous injection of 0.1% adrenaline solution in a dose of 0.2 ml per 100 g of body weight. 48 hours after administration of adrenaline, rats were subjected to euthanasia by overdose of ethyl aether. For monitoring the heart attack levels of aspartataminotransferase (AST), lactatdehydrogenase (LDH) and creatinfosphokinase (KFK) were estimated in blood serum of control and experimental rats 24 hours post adrenalin administration. Results. Biochemical studies of AST, LDH and KFK showed a significant increase of these enzymes activities in the experimental group animals. In particular, AST level increased 3.5 times, LDH – 2.7 times, KFK – 2.3 times in comparison with control group rats. 48 hours pos adrenalin administration it was documented local degenerative changes in cardiomyocytes, including cellular oedema, nuclear picnosis, disaggregation of myofibrils and destructive changes of intercalated disks. In the arterioles it was detected aggregation of blood cells and their adhesion to endothelium. Histochemical studies showed a decreased PAS-reactivity of intercalated discs accompanied with enhanced PAS labeling of areriolar internal elastic membrane and exposure of PAS-reactivity in the areas of necrotized cardiomyocytes adjusting blood vessels. Moreover, the accumulation of sulfated glycosoaminoglycan deposits was characteristic for vascular adventitia in experimental group animals. Conclusion. Our investigation revealed a significant increase in the blood serum levels of marker enzymes (AST, LDH and KFK) in the adrenalin-induced experimental myocardial infarction. Detected formation of leukocyte-erythrocyte blood clots apparently caused the disruption of blood supply and, consequently, dystrophic changes in cardiomyocytes, i.e. intercalated disks destruction, disorganization of myofibrils, oedematic conditions in stroma, the accumulation of PAS-positive material around necrotizing cardiomyocytes and sulfated glycosaminoglycans around the blood vessels.

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How to Cite

Nadraga, B. O., Yashchenko, A. M., & Lutsyk, A. D. (2019). Morphofunctional peculiarities of the rat heart in experimental myocardial infarction. Морфологія / Morphologia / Morfologìâ, 13(1), 32–37. https://doi.org/10.26641/1997-9665.2019.1.32-37

Issue

Vol. 13 No. 1 (2019)

Section

Статті

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Copyright (c) 2019 Nadraga B.O., Yashchenko A.M., Lutsyk A.D., Morphologia

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