Features of distribution and dynamics of the connective tissue fibers in the ventricles of rat’s myocardium in normal conditions and after intranatal injection of dexamethasone.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2018.4.99-103

Keywords:

connective tissue, heart, intranatal injection, dexamethasone

Abstract

Background. Any disturbance of the formation or organization of connective tissue such as undifferentiated connective tissue disease (UCTD) can lead to changes in extracellular matrix of the heart and deterioration of adaptive abilities in the future. The safety of using synthetic glucocorticoids in pregnant women is the subject of active discussions due to possible adverse effects of dexamethasone on the development of the fetus and, in particular, on connective tissue the heart. Objective. The purpose of this research is to study the features of distribution and dynamics of the connective tissue fibers in the ventricles of rat’s myocardium in normal conditions and after intranatal injection of dexamethasone. Methods. 144 hearts of laboratory rats were investigated. They were divided into 3 groups: the first group included intact rats, the second - control group, that get intrauterine injection of Sodium Chloride solution 0.9% 0.05 ml on the 18th day of antenatal development. The third group was formed by experimental animals, who were injected by 0.05 ml of dexametazone in a similar method. Serial sections were stained with orcein, van Gieson method and impregnation with silver carbonate by Lilly. Results. It was found that intrauterine administration of dexamethasone in rats causes a significant decrease of collagen fibers in the myocardium on 45-th day after birth comparison to control and intact groups by 30.8% and 29.1% respectively. In the structure of collagen fibers, the volume of type III fibers content at 14, 21 and 30 days were significantly higher in animals of intact and control groups than indexes of experimental group.Conclusion. Intranatal injection of dexamethasone may cause a violation in the development and lead to disorganisation of the connective tissue fibers in the ventricles of the heart. These changes may lead to development of UCTD and limitate the adaptive abilities of the heart in the future.

References

  1. Tyabut TD, Karatysh OM. [Undifferentiated connective tissue dysplasia]. Modern Rheumatology Journal. 2009;3(2):19-23. doi.org/10.14412/1996-7012-2009-534. Russian.
  2. Ushiki T. Collagen fibers, reticular fibers and elastic fibers. A comprehensive understanding from a morphological viewpoint. Arch Histol Cytol. 2002;65(2):109-26.
  3. Rog-Zielinska EA, Richardson RV, Denvir MA, Chapman KE. Glucocorticoids and foetal heart maturation; implications for prematurity and foetal programming. J Mol Endocrinol. 2014;52(2):125-35. DOI: 10.1530/JME-13-0204.
  4. Maresha SG, Yong L, Fuxia X, Thant L, Lubo Z. Dexamethasone treatment of newborn rats decreases cardiomyocyte endowment in the developing heart through epigenetic modifications. PLoS ONE. 2015;10(4):e0125033. DOI:10.1371/ journal.pone.0125033.
  5. Hryhorieva OA, Cherniavskyi AV. [Dynamics of Ventricular Wall and Interventricular Septum Thickness of Rat’s Heart in the Early Postnatal Period in Normal Conditions and after Intranatal Injection of Dexamethasone]. JMBS. 2018;3(3):12–5. DOI:10.26693/jmbs03.03.012. Ukrainian.
  6. Voloshin MA, Aravitskiy EO, Bogdanov PV, Chernyavskiy AV, Inventors; Zaporizhzhia State Medical University, assignee. Method of modeling of antenatal action of glucocorticoids. Ukraine patent UA 112288. 2016 Dec 12. Int. Cl. G09B 23/28, A61K 38/22, A61P 37/02. Ukrainian.
  7. Lillie RD. Patogistologicheskaya tehnika i prakticheskaya gistohimiya [Histopathologic technic and practical histochemistry]. Moscow: Mir; 1969. 645 p. Russian.
  8. Pires MM, Schepers A, van der Hoeven BL, de Vries MR, Lianne S.M. Jukema B, Quax PH. Histopathologic alterations following local delivery of dexamethasone to inhibit restenosis in murine arteries. Cardiovascular Research. 2005;68(3):415–24. DOI: 10.1016/j.cardiores.2005.06.015.

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How to Cite

Cherniavskyi, A. V. (2018). Features of distribution and dynamics of the connective tissue fibers in the ventricles of rat’s myocardium in normal conditions and after intranatal injection of dexamethasone. Морфологія / Morphologia / Morfologìâ, 12(4), 99–103. https://doi.org/10.26641/1997-9665.2018.4.99-103

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Vol. 12 No. 4 (2018)

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