Ultrastructural assessment of leiomyoma tissue and different layers of myometrium in patients with uterine leiomyoma during hypo- and hyperestrogenic states after different schemes of gonadotroping relising hormone agonists use.
DOI:
https://doi.org/10.26641/1997-9665.2011.4.25-36Keywords:
leiomyoma, agonist GnRH, myometrium, myocytes, electronic microsopy, pseudocapsula, stromaAbstract
In study conducted ultrastructural analysis of cells and extracellular matrix of myoma tissue and different layers of myometrium in 35 patients with uterine leyomioma in long-term and short-term correction of hormonal status before surgery gosereline acetate. Study of ultrastructural characteristics were performed on ultrathin sections for transmission electron microscope. Studies have shown that the ultrastructural characteristics of uterine leyomioma were similar to those of unchanged myometrium. The most characteristic feature of uterine fibroids is excessive extracellular matrix elements, arranged randomly. Under the action of a-GnRH there were atrophic changes in myocytes, which in most cases do not lead to cell death and is reversible: reducing the size of myocytes in both myomatous tissue and in normal myometrium, two-fold reduction in myofilaments amount in unchanged myometrium and in uterine myoma. Under the influence of hyperestrogenemy, along with increasing size of the nucleus, endoplasmic reticulum elements and number of ribosomes the size of myocytes was likely to increase in both myoma tissue and myometrium; increased the ratio of condensed chromatin to uncondensed one (in simple fibroids – 1.7 times, in cellular fibroids – 2.1 times); increase in 1.5 times the number of myofilaments both in unchanged myometrium and in uterine myoma; increase in number of mitochondria and increase of their size in myomatous tissue only caused mainly by by intracellular edema. Elements of extracellular matrix do not depend on short-term action of hyperestrogeny and mid-term action of hypoestrogeny, but the synthesis of new fibers is reduced under action of artificial menopause.
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