morphological assessment of state myomatous tissue and different layers of myometrium at patients with uterus leiomyoma at preoperative correction of the hormonal status by means of Zoladex.

Authors

  • M. V. Medvedev Dnipropetrovsk State Medical Academy, Ukraine

DOI:

https://doi.org/10.26641//1997-9665.2011.2.24-31

Keywords:

leiomyoma, Zoladex®, myometrium, myocytes, microvessels, stroma, morphometry

Abstract

In research was spent mорmorphometric analysis of cellular, stromal and microvascular components of myomatous tissue and different layers of myometrium at 35 patients with uterus leiomyoma during long-term and short-term preoperative correction of the hormonal status by means of Zoladex®. Studying of specific volume of microvessels and stroma, quantitative density of myocyte nuclei and a mitotic index of myocytes was spent on sections in the thickness by 1 microns, made of epoxy blocks. Researches have shown, that in the conditions of 3-month's medicamental menopause there is an authentic depression of specific volume of microvessels. In the conditions of the raised level of sexual steroids growth of contents of microvessels of myomatous nodes at the expense of dilating of arterioles and metarterioles lumen and thickening of their sides is observed. At simple myoma against a medicamental menopause the reduction of specific volume of stroma in myometriums submucous and vascular layers at that time in 1 week after introduction Zoladex the changes are not defined. In nodes of a cellular myoma in the conditions of long menopause it is observed more than double growth of relative contents of a stroma. In conditions hypohormonemia throughout 3 months there is depression of mitotic activity of nodal myocytes as a part of simple and cellular myoma. On the contrary, against hyperhormonemia in 1 week of Zoladex action essential is rising of a mitotic index of myocytes of cellular leiomyoma is observed.

References

  1. Автандилов Г. Г. Медицинская морфометрия : [руководство] / Г. Г. Автандилов. – М. : Медицина, 1990. – 384 с.
  2. Автандилов Г. Г. Основы количественной патологической анатомии / Г. Г. Автандилов. – М. : Медицина, 2002. – 240 с.
  3. Вихляева Е. М. Руководство по диагностике и лечению лейомиомы матки / Е.М. Вихляева. М. : МЕДпресс-информ, 2004. - 400 с.
  4. Лакин Г. Ф. Биометрия: Учеб. пособие для биол. спец. вузов. / Лакин Г. Ф. – [4-е изд., перераб. и доп.]. – М. : Высшая школа, 1990. – 352 с.
  5. Ланчинский В. И. Генетика и молекулярная биология миомы матки // Акушерство и гинекология. – 2004. – № 2. – С. 14-17.
  6. Сидорова И. Простая и пролиферирующая миома матки: морфологические особенности / И. Сидорова, О. Зайратьянц, С. Леваков, М. Баракова-Безуглая // Врач. – 2004. – № 4. – С. 30-31.
  7. Татарчук Т. Ф. Cучасні принципи діагностики та лікування лейоміоми матки / Т. Ф. Татарчук, Н. В. Косей // Сімейна медицина. – 2005. – № 4. – С. 67-73.
  8. Тихомиров А. Л. Миома матки / А. Л. Тихомиров, Д. М. Лубнин. – М. : МИА, 2006. – 174 с.
  9. Clinical management of uterine sarcomas / F. Amant, A. N. Coosemans, M. Debiec-Rychter [et al.] // The Lancet Oncology. – 2009. – Vol. 10, № 12. – P. 1188-1198.
  10. Comparison between bisection/morcellation and myometrial coring for reducing large uteri during vaginal hysterectomy or laparoscopically assisted vaginal hys-terectomy: result of a randomized prospective study / I. Nazah, F. Robin, J. Jais [et al.] // Acta Obstet. Gynaecol. Scand. – 2003. – Vol. 82. – P. 1037-1042.
  11. Correlation between shrinkage of uterine leiomyoma treated with buserelin acetate and histopathologic findings of biopsy specimen before treatment / N. Kawamura, F. Ito, T. Ichimura [et al.] // Fertil. Steril. – 1997. – Vol. 68. – P. 632-636.
  12. Crow J. Morphological changes in uterine leiomyomas treated by GnRH-agonist goserelin / J. Crow, R. L. Gardner, G. McSweeney, R. W. Shaw // Int. J. Gynecol. Pathol. – 1995. – Vol. 14. – P. 235-242.
  13. Diagnostic accuracy of needle biopsy of uterine leiomyosarcoma / S. Shi-bata, N. Kawamura, F. Ito [et al.] // Oncol. Rep. – 2000. – Vol. 7. – P. 595-597.
  14. Hoff de R. T. Quantative microscopy / R. T. de Hoff, F. N. Rhines. – N.Y. : McGraw-Hill, 1968. – 422 p.
  15. Humphrey Ch. D. A simple methilene blue - azure II - basic fuchsin stain for epoxy-embedded tissue sections / Ch. D. Humphrey, F. E. Pittman // Stain Technol. – 1974. – Vol. 49. – P. 9-14.
  16. Lin J. F. Uterine sarcoma 2008 / J. F. Lin and B. M. Slomovitz // Curr. Oncol. Rep. – 2008. – Vol. 10, № 6. – P. 512-518.
  17. Morphometric and electronmicroscopic analyses of the effects of gonadotropin-releasing hormone agonists on uterine leiomyomas / T. Kalir, M. Goldstein, P. Dottino [et al.] // Arch. Pathol. Lab. Med. – 1998. – Vol. 122. – P. 442-446.
  18. Narayan A. Uterine artery embolization versus abdominal myomectomy: A long-term clinical outcome comparison / A. Narayan // J. Vasc. Interventional Radiol. – 2010. – Vol. 21. – P. 1011-1014.
  19. Parazzini F. The epidemiology of uterine leiomyomata. Uterine leiomyomata pathogenesis and management / F. Parazzini, F. Chiaffarino; ed. by Ivo Brosens. – London; New York: Taylor and Francis, 2006. – P. 3-9.
  20. The expression of Ki-67, p53, estrogen and progesterone receptors affecting survival in uterine leiomyosarcomas. A clinicopathologic study / S. E. Akhan, E. Yavuz, A. Tecer [et al.] // Gynecologic Oncology. – 2005. – Vol. 99, № 1. – P. 36–42.

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Medvedev, M. V. (2011). morphological assessment of state myomatous tissue and different layers of myometrium at patients with uterus leiomyoma at preoperative correction of the hormonal status by means of Zoladex. Морфологія / Morphologia / Morfologìâ, 5(2), 24–31. https://doi.org/10.26641//1997-9665.2011.2.24-31

Issue

Vol. 5 No. 2 (2011)

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Статті

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Copyright (c) 2011 Medvedev M.V., Morphologia

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