Iinvestigation of neural cells cryopreserved aggregates transplantation efficiency separately and together with mesenchymal stem cells for the treatment of intracerebral hemorrhage in rats.

Authors

DOI:

https://doi.org/10.26641/1997-9665.2018.3.79-84

Keywords:

intracerebral haemorrhage, rats, aggregates of neural cells, mesenchymal stem cells, cellular therapy, leukocytes, morphometry

Abstract

Background. The Intracerebral haemorrhage is accompanied by severe complications and high mortality. There are currently no effective methods of treating this disease. Objective. to study the therapeutic efficiency of transplantation of cryopreserved neural cells in three-dimensional aggregates in combination with mesenchymal stem cells at the course of the pathological process in rats after intracerebral haemorrhage. Methods: isolation and cultivation of cells, cryopreservation, stereotaxis, transplantation, morphometry. Results. Experimental animals were injected with aggregates of neural cells, and aggregates together with mesenchymal stem cells. Control animals were injected with DMEM/F12. It has been established that intraventricular administration of cryopreserved aggregates of neural cells together with mesenchymal stem cells to rats with intracerebral hemorrhage significantly reduces the number of leukocytes starting from 6 days after transplantation in comparison with control. In the same experimental group the relative amount of neutrophils significantly decreased by 23.9%, and the relative amount of lymphocytes increased by 25.6% compared to the control on 1-st day after transplantation. In the group of neural cells aggregates transplantation the area of glial scars decreased by 34.9% compared to the control, and the ratio of the injured striatum diameter to the healthy one increased by 33.9%. Conclusion. The results of the study show that transplantation of neural cells aggregates together with mesenchymal stem cells is more effective in reducing inflammation in intracerebral haemorrhage, while the administration of aggregates of neural cells alone is more effective in the long term.

References

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Zolotko, K. N., & Sukach, A. N. (2018). Iinvestigation of neural cells cryopreserved aggregates transplantation efficiency separately and together with mesenchymal stem cells for the treatment of intracerebral hemorrhage in rats. Морфологія / Morphologia / Morfologìâ, 12(3), 79–84. https://doi.org/10.26641/1997-9665.2018.3.79-84

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Vol. 12 No. 3 (2018)

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