Influense of the effect of experimental diabetes mellitus on the protein expression of apoptotic regulators Bcl-2 and p53 in the lymphoid follicles of the spleen.

Authors

  • I. Grinevich Zaporizhzhia State Medical University, Ukraine
  • A. Kamyshny Zaporizhzhia State Medical University, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2010.4.19-23

Keywords:

spleen, diabetes mellitus, Bcl-2, p53

Abstract

The problem of investigation of molecular mechanisms of apoptosis and its role in autoimmune disease, including diabetes mellitus, became one of the most difficult and actual among the medical sciences last years. Under the experiment the effect of experimental diabetes mellitus on the intensity of protein expression of apoptotic regulators Bcl-2 and p53 in the spleen of rats was investigated. To detect Bcl-2 and p53 positive-cells we used immunohistochemical technique of indirect immunofluorescence with monoclonal antibodies to Bcl-2 and p53. It was found that the development of experimental diabetes mellitus is accompanied by unidirectional tendency to increase the number of p53- and Bcl-2-positive cells in lymphoid follicles of the spleen with a preferential decrease in the concentration of these protein in immune cells.

References

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How to Cite

Grinevich, I., & Kamyshny, A. (2010). Influense of the effect of experimental diabetes mellitus on the protein expression of apoptotic regulators Bcl-2 and p53 in the lymphoid follicles of the spleen. Морфологія / Morphologia / Morfologìâ, 4(4), 19–23. https://doi.org/10.26641/1997-9665.2010.4.19-23

Issue

Vol. 4 No. 4 (2010)

Section

Статті

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