Immunohistochemical evaluation of leiomyoma tissue and normal myometrium in patients with uterine leiomyoma during hypo- and hyperestrogenic conditions caused by of use of gonadotropin-releasing hormone analogues.

Authors

  • M. V. Medvedev ДЗ «Дніпропетровська медична академія МОЗ України», Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2012.1.43-49

Keywords:

leiomyoma, agonist GnRH, immunohistochemical analysis

Abstract

In study immunohistochemical analysis of markers of proliferation, apoptosis, extracellular matrix degradation, angiogenesis and steroid hormone receptors in myomatous tissue and myometrium was conducted in 62 patients with uterine leiomyomas during long-term and short-term correction of hormonal status by gosereline acetate.Studies have shown that pseudocapsule of fibroids at immunohistochemical level is unchanged myometrium.For leiomyoma tissue compared to myometrium were characteristic greater expression of steroid hormone receptors and aromatase, inhibition of apoptosis, greater expression of angiogenic factors compared to unchanged myometrium.Under the action of GnRH-a direct and mediated by reduction of estradiol occurred molecular changes, which cover almost all parts of the pathogenesis of fibroids and include inhibition of expression of estrogen receptor and aromatase synthesis, enhancing of expression of progesterone receptors, inhibition of proliferation and angiogenesis, accelerated degradation of ECM. Under the influence of hyperestrogeny caused by flare-up effect of GnRH-a and direct peripheral effect of GnRH analogs progression of uterine leiomyoma provides increased sensitivity of uterine myocytes to ischemia.Despite the obvious role of antiapoptotic factor bcl-2 in the pathogenesis of uterine fibroids, short-and long-term hormonal treatment with GnRH-a does not lead to significant changes in its expression.

References

  1. Беженарь В. Ф. Современная стратегия и хирургическая техника при лапароскопической миомэктомии / В. Ф. Беженарь, Н. С. Медведева, Э. К. Айламазян // Здоровье женщины. − 2008. − Т. 36, № 4. − С. 22-26.
  2. Медведєв М. В. Нова схема перед- та післяопераційного застосування аналогів ГнРГ у жінок з лейоміомою матки / М. В. Медведєв // Здоровье женщины. − 2011. − Т. 63, № 7. − С. 32-37.
  3. Місце імуногістохімічних методів у диференційній діагностиці гладком’язових пухлин матки / І. С. Шпонька, В. О. Потапов, М. В. Медведєв, Г. С. Гончарова // Медичні перспективи. − 2009. − Т. 14, № 2. − С. 39-46.
  4. Татарчук Т. Ф. Cучасні принципи діагностики та лікування лейоміоми матки / Т. Ф. Татарчук, Н. В. Косей // Сімейна медицина. – 2005. – № 4. – С. 67-73.
  5. Тихомиров А. Л. Миома матки / А. Л. Тихомиров, Д. М. Лубнин. – М. : МИА, 2006. – 174 с.
  6. De Leo V. A benefit-risk assessment of medical treatment for uterine leiomyomas / V. De Leo, G. Morgante, A. La Marca // Drug Saf. − 2002. − Vol. 25, № 11. − P. 759-779.
  7. Lethaby A. Efficacy of pre-operative gonadotrophin hormone releasing analogues for women with uterine fibroids undergoing hysterectomy or myomectomy: a systematic review / A. Lethaby, B. Vollenhoven, M. Sowter // Bjog. − 2002. − Vol. 109, № 10. − P. 1097-1108.
  8. Cellular proliferation, estrogen receptor, progesterone receptor, and bcl-2 expression in GnRH agonist-treated uterine leiomyomas / K. Vu, D. L. Greenspan, T. C. Wu [et al.] // Hum Pathol. − 1998. − Vol. 29, № 4. − P. 359-363.
  9. Effects of treatment with gonadotropin releasing hormone agonist on the uterine leiomyomata structure / N. Bozzini, C. J. Rodrigues, D. A. Petti [et al.] // Acta Obstet Gynecol Scand. − 2003. − Vol. 82, № 4. − P. 330-334.
  10. Estrogen and progesterone receptor content of GnRH analogue pretreated and untreated uterine leiomyomata / P. A. Regidor, M. Schmidt, R. Callies [et al.] // Eur J Obstet Gynecol Reprod Biol. − 1995. − Vol. 63, № 1. − P. 69-73.

Downloads

How to Cite

Medvedev, M. V. (2012). Immunohistochemical evaluation of leiomyoma tissue and normal myometrium in patients with uterine leiomyoma during hypo- and hyperestrogenic conditions caused by of use of gonadotropin-releasing hormone analogues. Морфологія / Morphologia / Morfologìâ, 6(1), 43–49. https://doi.org/10.26641/1997-9665.2012.1.43-49

Issue

Vol. 6 No. 1 (2012)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.