Accessible morphohistochemical labs-on-a-chip based on different counting chambers' grids: microfluidic morphodynamical workstations.

Authors

  • O. V. Gradov Лабораторія нейронної структури мозку Наукового Центру Неврології РАМН, Москва, РФ Institute of Mathematical Statistics, Maryland, USA SIAM Activity Group of Life Science, Philadelphia, USA, Russian Federation
  • A. V. Notchenko Лабораторія нейронної структури мозку Наукового Центру Неврології РАМН, Москва, РФ МДТУ ім. Н.Е.Баумана, Москва, РФ, Russian Federation

DOI:

https://doi.org/10.26641/1997-9665.2012.1.5-19

Keywords:

lab-on-a-chip, haemocytometer, counting chambers, hematimeter, mathematical morphology, computer morphometry, 3D luminance surface, digital morphogenesis

Abstract

An accessible design of autonomous labs on the chip which do not require a special reader is developed. The proposed device uses hemocytometric counting chambers for determination of concentration of cells, isolated by a special device for cell sedimentation. A system of automated Rf-identification of chambers in the long-term storage is proposed which provides new morphometric data at various stages of cultivation or biomonitoring. A new diffraction method of calculation and fingerprinting of cellular structures in varying environmental conditions is described. Experimental data on the pilot testing of Fuchs-Rosenthal, Buerker, Neubauer, Makler and Thoma chambers for the described technique is given. The applicability of these devices for coherent-densitometric indication either of concentration dynamics in cell culture or suspension or morphogenetic processes in them for laminar conditions is shown. A self-learning program for cellular pattern recognition and relational cytophotometry based on lab on the chip with an object-micrometric calibration relative to the counting net is demonstrated. A hybrid in vitro / in silico method of morphogenesis monitoring in cell cultures is introduced.

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How to Cite

Gradov, O. V., & Notchenko, A. V. (2012). Accessible morphohistochemical labs-on-a-chip based on different counting chambers’ grids: microfluidic morphodynamical workstations. Морфологія / Morphologia / Morfologìâ, 6(1), 5–19. https://doi.org/10.26641/1997-9665.2012.1.5-19

Issue

Vol. 6 No. 1 (2012)

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