Inhibition of pancreatic stellate cell activation by the vitamin A and vitamin E as a therapy for prevention fibrogenesis in experimental chronic alcoholic pancreatitis.

Authors

  • M. E. Nichitaylo Национальный институт хирургии и трансплантологии им. академика А.А.Шалимова, Киев, Ukraine
  • D. A. Kravchenko Национальный институт хирургии и трансплантологии им. академика А.А.Шалимова, Киев, Ukraine
  • E. B. Medvetskii Национальный институт хирургии и трансплантологии им. академика А.А.Шалимова, Киев, Ukraine
  • I. S. Shpon’ka ГУ «Днепропетровская медицинская академия МЗ Украины», Ukraine
  • I. M. Savitskaia Национальный институт хирургии и трансплантологии им. академика А.А.Шалимова, Киев, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2012.2.34-42

Keywords:

pancreas, chronic pancreatitis, distal pancreatectomy, pancreatic stellate cells, vitamin A, vitamin E

Abstract

The aim of the study was to investigate the effects of Vitamin A and Vitamin E on activity of pancreatic stellate cells and fibrosis changes in pancreas after distal pancreatectomy in rats with experimental alcohol-induced chronic pancreatitis. Simultaneously Vitamin A and Vitamin E were administered after distal pancreatectomy in rats with experimental alcohol-induced chronic pancreatitis. The animals were treated with Vitamin A at the dose of 33000 IU/kg body weight per day and Vitamin E at the dose of 100 mg/kg body weight per day for three weeks (21 days) after operation.To estimate the efficacy of the treatment on activity and numbers of pancreatic stellate cells the immunohistochemical investigation was made with alpha-smooth muscle actin, desmin, vimentin, glial fibrillary acidic protein (GFAP), matrix metalloproteinase 1 (MMP1), tissue inhibitor of metalloproteinase 2 (TIMP2) using.The treatment of rats after operation with vitamin A and vitamin E inhibited activity of pancreatic stellate cells and characterized by significant decreasing of the alpha-smooth muscle actin, Desmin, Vimentin, MMP1 and TIMP2 expression. The ratio of MMP1/TIMP2 was greater in the group with treatment then in the control group. This therapy had a trend to decrease the expression of GFAP and alleviate the fibrotic changes in pancreas.

References

  1. Органосохраняющие технологии при хроническом панкреатите головки поджелудочной железы / С. Д. Добров, А. С. Полякевич, Е. М. Благитко [и др.] // Анналы хирургической гепатологии. – 2007. - № 12. - С. 96-102.
  2. Angiotensin II signaling through the AT1a and AT1b receptors does not have a role in the development of cerulein-induced chronic pancreatitis in the mouse / B. Ulmasov, Z. Xu, V. Talkad [et al.] // Am. J. Physiol. Gastrointest. Liver Physiol. – 2010. – Vol. 299. – P. 70-80.
  3. Apte M. The fibrosis of chronic pancreatitis: new insights into the role of pancreatic stellate cells / M. Apte, R. Pirola, J. Wilson // Antioxid Redox. Signal. – 2011. – Vol. 15, № 10. – P. 2711-2722.
  4. Chronic Pancreatitis: Evolving Paradigms / R. Talukdar, N. Saikia, D. K. Singal [et al.] // Pancreatology. – 2006. – Vol. 6. – P. 440–449.
  5. Development of an animal model of chronic alcohol-induced pancreatitis in the rat / K. Hiroshi, M. Nakagami, I. Rusyn [et al.] // Am. J. Physiol. Gastrointest. Liver Physiol. – 2001. – Vol. 280. – P. 1178–1186.
  6. Duodenum-Preserving Subtotal and Total Pancreatic Head Resections for Inflammatory and Cystic Neoplastic Lesions of the Pancreas / H. G. Beger, B. M. Rau, F. Gansauge [et al.] // J. Gastrointest. Surg. – 2008. – Vol. 12. – P. 1127-1132.
  7. Effect of surgery for chronic pancreatitis on pancreatic function: Pancreaticojejunostomy and duodenum-preserving resection of the head of the pancreas / S. Maartense, M. Ledeboer, W. A. Bemelman [et al.] // Surgery. – 2004. – Vol. 135. – P. 125-130.
  8. Expressions of matrix metalloproteinases in early-stage oral squamous cell carcinoma as predictive indicators for tumor metastases and prognosis / A. Katayama, N. Bandoh, K. Kishibe [et al.] // Clinical cancer research. – 2004. - Vol. 10, № 1. – P. 634-640.
  9. Frey C. F. Surgery of chronic pancreatitis / C. F. Frey, D. K. Andersen // The American Journal of Surgery. – 2007. – Vol. 194. – P. 53-60.
  10. Li X. C. α-Tocopherol treatment ameliorates chronic pancreatitis in an experimental rat model induced by trinitrobenzene sulfonic acid / X. C. Li, X. L. Lu, H. H. Chen // Pancreatology. – 2011. – Vol. 10, № 11. – P. 5-11.
  11. Long term follow-up of a randomized trial comparing the Beger and Frey procedures for patients suffering from chronic pancreatitis / T. Strate, Z. Taherpour, C. Bloechle [et al.] // Ann. Surg. – 2005. – Vol. 241. – P. 591- 598.
  12. Long-term metabolic results after pancreatic resection for severe chronic pancreatitis / T. Berney, T. Rudisuhli, J. Oberholzer [et al.] // Arch. Surg. – 2000. – Vol. 135. – P. 1106-1111.
  13. Protective role of angiotensin II type 2 receptor signaling in a mouse model of pancreatic fibrosis / B. Ulmasov, Z. Xu, L. H. Tetri [et al.] // Am. J. Physiol. Gastrointest. Liver Physiol. – 2009. – Vol. 296. – P. 284-294.
  14. Regulation of pancreatic stellate cell function in vitro: biological and molecular effects of all-trans retinoic acid / R. Jaster, I. Hilgendorf, B. Fitzner [et al.] // Biochem Pharmacol. – 2003. – Vol. 66, №4. – P. 633-641.
  15. Risk factors for diabetes mellitus in chronic pancreatitis / D. Malka, P. Hammel, A. Sauvanet [et al.] // Gastroenterology. – 2000. – Vol. 119. – P. 1324–1332.
  16. Shimizu K. Mechanisms of pancreatic fibrosis and applications to the treatment of chronic pancreatitis / K. Shimizu // J. Gastroenterol. – 2008. – Vol. 43, № 11. – P. 823-832.
  17. Talukdar R. Pancreatic stellate cells: new target in the treatment of chronic pancreatitis / R. Talukdar, R. K. Tandon // Journal of Gastroenterology and Hepatology. – 2008. – Vol. 23. – P. 34-41.
  18. Vitamin A inhibits pancreatic stellate cell activation: implications for treatment of pancreatic fibrosis / J. A. Mccarroll, P. A. Phillips, N. Santucci [et al.] // Gut. – 2006. – Vol. 55. – P. 79-89.

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How to Cite

Nichitaylo, M. E., Kravchenko, D. A., Medvetskii, E. B., Shpon’ka, I. S., & Savitskaia, I. M. (2012). Inhibition of pancreatic stellate cell activation by the vitamin A and vitamin E as a therapy for prevention fibrogenesis in experimental chronic alcoholic pancreatitis. Морфологія / Morphologia / Morfologìâ, 6(2), 34–42. https://doi.org/10.26641/1997-9665.2012.2.34-42

Issue

Vol. 6 No. 2 (2012)

Section

Статті

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