Age features of eyeball envelopes structure.

Authors

  • N. A. Ulyanova Одесский национальный медицинский университет, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2014.1.95-98

Keywords:

sclera, retina, rat

Abstract

Background. The absence of adequate experimental model of myopia is the actual problem in pathophysiological investigation of the myopia progression. Studies of the chick eye have formed the basis for several hypotheses of myopia development. The most pathogenically valid animal model of myopia is a deprivation model. Before introduction of this model in mammals, in particular rats, it is necessary to investigate the dynamics of age morphological changes in sclera and retina. Objective. To determine the age-related features of the sclera and retina in intact rats. Methods. The sclera and retina were investigated by optic microscopy method on the 14th, 21st, 30th, 40th, 50th, 60th, 70th, 90th days after birth. Results. It was determined that sagittal, vertical and horizontal sizes of rats eyes increase more intensively at the age period from the 14th to the 30th day. At this time the maximal number of fibroblasts was observed in sclera. The total amount of these cells decreases after 40 days of postnatal ontogenesis. At the same time changes in thickness and compactness of retina layers were detected. At the age period from the 14th to the 30th day definitive scleral tissue architecture is not yet formed, part of collagen fibrils are organized in bundles, part of them are loosely arranged. On the 90th day all collagen fibrils of scleral stroma and retina are arranged. Conclusion. The highest intensity of sclera and retina restructuring is observed between the 14th and 30th days of postnatal ontogenesis. This period could be considered as optimal for experimental modeling of myopia in rats.

References

  1. Flitcroft DI. Is myopia a failure of homeostasis? Exp Eye Res. 2013 Sep;114:16-24. doi: 10.1016/j.exer.2013.02.008. Cited in: PubMed; PMID: 23454097.
  2. Mathis U, Schaeffel F. Transforming growth factor-beta in the chicken fundal layers: an immunohistochemical study. Exp Eye Res. 2010 Jun;90(6):780-90. doi: 10.1016/j.exer.2010.03.014. Cited in: PubMed; PMID: 20350541.
  3. Zhong X, Ge J, Smith EL 3rd, Stell WK. Image defocus modulates activity of bipolar and amacrine cells in macaque retina. Invest Ophthalmol Vis Sci. 2004 Jul;45(7):2065-74. Cited in: PubMed; PMID: 15223778.
  4. Mishalov VD, Chaikovskiy YuB, Tverdokhleb IV. [About legal, legislative, ethical standards and requirements at performance scientific morphological researches]. Morphologia. 2007;1(2):108-15. Ukrainian.
  5. Sarkisov DS, Perov YuL, editors. [Microscopic technique: manual]. Moscow: Meditsina; 1996. 544 p. Russian.
  6. Glantz SA. Mediko-biologicheskaya statistika [Primer of biostatistics]. 4th ed. Danilov YuA, translator. Buzikashvili NYe, Samoylov DV, editors. Moscow: Praktika; 1998. 459 p. Russian.
  7. Avetisov ES. Blizorukost [Myopia]. Moscow: Meditsina; 2002. 288р. Russian.

Downloads

How to Cite

Ulyanova, N. A. (2014). Age features of eyeball envelopes structure. Морфологія / Morphologia / Morfologìâ, 8(1), 95–98. https://doi.org/10.26641/1997-9665.2014.1.95-98

Issue

Vol. 8 No. 1 (2014)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.