Morphological and functional features of the thymus of rats of different age periods in health and at experimental immunostimulation.

Authors

  • I. V. Bobrysheva ГЗ «Луганский государственный медицинский университет», Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2013.4.11-17

Keywords:

thymus, rats, immunostimulation

Abstract

Background. Data on the structure of the thymus of white rats with experimental immunostimulation in age aspect presented only in a few publications. Objective. Morphofunctional features of the thymus of white male rats of three age periods: pubertal, reproductive and of expressed age-related changes, administered immunomodulator imunofan were studied. Methods. Features of the histological structure of the thymus were studied using an image analyzer based on microscope Olympus CX-41. Areas of subcapsular zone, the cortex and medulla, the total number of cells, lymphocytes and epitelioreticular cells conditional on a standard area (104 μm2) were determined; limphoepitelial index was calculated. Results. The thymus has specific morphological features of the structure in each age group of animals. The thymus of rats of pubertal period has the highest morphometric parameters which somewhat decreased in animals of reproductive period. Animals with expressed age-related changes have signs of age involution of the thymus (replacing of parenchyme of the organ by adipose and connective tissue, a sharp decline in morphometric parameters of morphofunctional zones). Conclusion. Application of imunofan causes unidirectional positive changes of morphometric parameters of the thymus in the rats of all age periods on 7-60 day of observation. In animals of the reproductive period and the period of expressed age-related changes injections of immunomodulator cause a decrease in the intensity of the involutive processes in the thymus.

References

  1. Мельник Н. О. Реактивні зміни органів імунного захисту за умов демієлінізації та ремієлінізації / Н. О. Мельник, Ю. Б. Чайковський // Морфологія. – 2007. – Т. 1, № 1. – С. 89-93.
  2. Melnik NO, Chaykovsky YuB. [Reactive changes of the immune system in conditions of demyelination and remyelination]. Morphologіa. 2007;1(1):89-93. Ukrainian.
  3. Boehm T. Thymus development and function / T. Boehm // Cur. Opin. Immun. – 2008. – Vol. 20, № 2. – Р. 178-184.
  4. Boehm T. Thymus development and function. Curr. Opin. Immunol. 2008;20(2):178-84.
  5. Кащенко С. А. Органометрические особенности строения тимуса белых крыс после иммуностимуляции и иммуносупрессии / С. А. Кащенко, А. А. Захаров // Укр. жур. клін. та лаб. мед. – 2009. – Т. 4, № 3. – С. 50-52.
  6. Kashchenko SA, Zakharov AA. [Organometric structural features of the white rat thymus after immunostimulation and immunosuppression]. Ukrainsky journal klіnіchnoi i laboratornoi mediciny. 2009;4(3):50-2. Russian.
  7. Волошин В. М. Вивчення інгаляційного впливу епіхлоргідрину на органометричні показники тимуса в експерименті / В. М. Волошин // Клінічна анатомія та оперативна хірургія. – 2012. - Т. 11, № 1. – С. 35-38.
  8. Voloshin VM. [The study of inhalation exposure to epichlorohydrin orhanometric performance of the thymus in the experiment]. Klіnіchna anatomіya ta operativna hіrurgіya. 2012;11(1):35-8. Ukrainian.
  9. Морфологические изменения тимуса после применения полиоксидония / Г. Ю. Стручко, Л. М. Меркулова, Е. В. Москвичев [и др.] // Фундам. иссл. – 2012. – № 5, Ч. 1. – С. 197-202.
  10. Struchko GYu, Merkulova LM, Moskvichev EV. [Morphological changes in the thymus after applying of polyoxidonium]. Fundamentalniye issledovzniya. 2012;5(1):197-202. Russian.
  11. Лебедев В. В. Имунофан – синтетический пептидный препарат нового поколения / В. В. Лебедев, В. И. Покровский // Вестник РАМН. - 1999. - № 4. - С. 56-61.
  12. Lebedev VV, Pokrovsky VI. [Imunofan – synthetic peptide drug of the new generation]. Vestnik RAMN. 1999;4:56-61. Russian.
  13. Шаршембиев Ж. А. Особенности строения тимуса при воздействии полиоксидония / Ж. А. Шаршембиев // Морфология. – 2004. – Т. 125, № 2. – С. 59–60.
  14. Sharshembiev JA. [Features of the structure of the thymus under the influence of polyoxidonium]. Morfologiya. 2004;125(2):59-60. Russian.
  15. Effect of intrauterine exposure of murino fetus to cyclophosphamide on development of thymus / G. V. Prakash, S. M. Singh, M. P. Singh [et al.] // Immunoph. Immunotox. – 2007. – Vol. 29, № 1. – P. 17-30.
  16. Prakash GV, Singh SM, Singh MP et al. Effect of intrauterine exposure of murino fetus to cyclophosphamide on development of thymus. Immunopharmacology and Immunotoxicology. 2007;29(1):17-30.
  17. Кащенко С. А. Морфологическая реактивность тимуса белых крыс при иммуностимуляции / С. А. Кащенко // Укр. мед. альм. – 2004. – Т. 2, № 1. – С. 15–19.
  18. Kashchenko SA. [Morphological reactivity of thymus on white rats under immunostimulation]. Ukrainskiy medichniy almanah. 2004;2(1):15-9. Russian.
  19. Каулен Д. Р. Антигенные маркёры и субпопуляции лимфоидных клеток крыс / Д. Р. Каулен, А. В. Пронин, В. В. Хоробрых // Иммунология. - 1981. - № 3. - С. 5-12.
  20. Kulen DR, Pronin AV, Khorobrikh VV. [Antigenic markers and lymphoid cell subpopulations in rats]. Immunologiya. 1981;3:5-12. Russian.
  21. Волошин М. А. Основи імунології та імуноморфології / М. А. Волошин, Ю. Б. Чайковський, О. Г. Кущ. – Запоріжжя, Київ, 2010. - 170 с.
  22. Voloshin MA, Chaykovsky YuB, Kush OG. authors; Osnovy imunologiyi ta imunomorfologiyi [Bases of іmmunology and іmmunomorphology]. Zaporizhzhya-Kyiv; 2010.170 p. Ukrainian.

Downloads

How to Cite

Bobrysheva, I. V. (2013). Morphological and functional features of the thymus of rats of different age periods in health and at experimental immunostimulation. Морфологія / Morphologia / Morfologìâ, 7(4), 11–17. https://doi.org/10.26641/1997-9665.2013.4.11-17

Issue

Vol. 7 No. 4 (2013)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.