The influence of xenogenic cerebrospinal fluid over the parenchyma of rat's lungs after single total irradiation.

Authors

  • D. V. Shatov ГУ «Крымский государственный медицинский университет имени С.И. Георгиевского», Симферополь, Ukraine
  • V. S. Pikalyuk ГУ «Крымский государственный медицинский университет имени С.И. Георгиевского», Симферополь, Ukraine
  • V. V. Shalanin ГУ «Крымский государственный медицинский университет имени С.И. Георгиевского», Симферополь, Ukraine
  • T. S. Shimkus ГУ «Крымский государственный медицинский университет имени С.И. Георгиевского», Симферополь, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2014.2.77-88

Keywords:

cerebrospinal fluid, parenchyma of lungs, morphometry, irradiation, correction

Abstract

Background. One of the results of negative influence of irradiation is the radiational injury of lungs, which manifests itself by radiational pneumonitis and pulmonary fibrosis. The medicines of phytogenous and animal origin, microelements and synthetic analogs of natural organism protection's substances, high-molecular compounds, vaccines, cytokines and their inductors are used for early therapy of these complications. As one of such remedies one can use the xenogenic cerebrospinal fluid of cows since its usage shows good effects over the system of hematopoiesis at treatment of medullar form of acute radiation sickness. Objective. Studying the influence of xenogenic cerebrospinal fluid over the lung's parenchyma of the rats, which were exposed to single total irradiation. Methods. The research was performed on 48 mature aged (5 months) Wistar rats of both sexes. The animals were divided into following groups: the placebo group, the group, which was getting xenogenic cerebrospinal fluid, the irradiated group, which was getting placebo, and the irradiated group, which was getting cerebrospinal fluid. The lungs were later histologically processed and colored by hematoxylin-eosin and picrofucsine. During the microscopy the percentage of the areas with intact parenchyma, emphysema, dystelektases and hemorrhages, and also the intensity of the collagen fibers were estimated. Results. The triple introduction of the xenogenic cerebrospinal fluid to the animals, which were undergone the irradiation, have reduced the percentage of emphysematous areas by 8,86% (р<0,01) and the haemorrhagic areas by 2,44% (р<0,001) at the expense of increase of the percentage of intact parenchyma by 7,21% (р<0,01) and dystelectatic areas by 4,09% (р<0,001). At the tenfold introduction the percentage of the areas with the intact parenchyma has grown by 11,28% (р<0,001) and the percentage of dystelectatic areas by 4,51% (р<0,001), while the emphysematous areas decreased by 14,42% (р<0,001) and the haemorrhagic areas – by 1,39% (р<0,001). The most pronounced changes were observed at the 30-day experiment. Conclusion. The introduce of xenogenic cerebrospinal fluid to the animals, which were undergone the single total gamma irradiation by 5 Gy, leads to the decrease of the percentage of emphysematous areas and the increase of the intact parenchyma's and dystelectatic areas' percentage, which are more pronounced under the tenfold introduce of CSF (while the normal characteristics of the lung's morphometry are not reached). These changes are accompanied by reduced collagen formation in the lungs.

References

  1. Vasić L, Durdević P. [Radiation-induced lung damage - etiopathogenesis, clinical features, imaging findings and treatment]. Med Pregl. 2012;65(7-8):319-25. Serbian. PMID: 22924253.
  2. Ghafoori PL, Marks LB, Vujaskovic Z, Kelsey CR. Radiation-induced lung injury. Assessment, management, and prevention. Oncology. 2008;22(1):37-47; discussion 52-3. PMID: 18251282.
  3. Molthen RC, Wu Q, Fish BL, Moulder JE, Jacobs ER, Medhora MM. Mitigation of radiation induced pulmonary vascular injury by delayed treatment with captopril. Respirology. 2012;17(8). DOI: 10.1111/j.1440-1843.2012.02247.x. PMCID: PMC3848310. NIHMSID: NIHMS526628.
  4. Gao F, Fish BL, Moulder JE, Jacobs ER, Medhora M. Enalapril mitigates radiation-induced pneumonitis and pulmonary fibrosis if started 35 days after whole-thorax irradiation. Radiat Res. 2013;180(5):546-52. DOI: 10.1667/RR13350.1. PMID: 24131041. PMCID: PMC3895940.
  5. Mahmood J, Jelveh S, Calveley V, Zaidi A, Doctrow SR, Hill RP. Mitigation of lung injury after accidental exposure to radiation. Radiat Res. 2011;176(6):770-80. PMCID: PMC3252890 NIHMSID: NIHMS342813.
  6. Mahmood J, Jelveh S, Calveley V, Zaidi A, Doctrow SR, Hill RP. Mitigation of radiation-induced lung injury by Genistein and EUK-207. Int J Radiat Biol. 2011; 87(8):889-901. DOI:10.3109/09553002.2011.583315. PMCID: PMC315580. NIHMSID: NIHMS306825.
  7. Jang SS, Kim HG, Lee JS, Han JM, Park HJ, Huh GJ, Son CG. Melatonin reduces X-ray radiation-induced lung injury in mice by modulating oxidative stress and cytokine expression. Int J Radiat Biol. 2013;89(2):97-105. DOI: 10.3109/09553002.2013.734943.
  8. Rübe CE, Wilfert F, Uthe D, Schmid KW, Knoop R, Willich N, Schuck A, Rübe C. Modulation of radiation-induced tumour necrosis factor α (TNF-α) expression in the lung tissue by pentoxifylline. Radiotherapy & Oncology. 2002;64(2):177-87.
  9. Wang H, Yang YF, Zhao L, Xiao FJ, Zhang QW, Wen ML, Wu CT, Peng RY, Wang LS. Hepatocyte growth factor genemodified mesenchymal stem cells reduce radiation-induced lung injury. Hum Gene Ther. 2013;24(3):343-53. DOI: 10.1089/hum.2012.177. PMID: 23458413.
  10. Bese NS, Munzuroglu F, Uslu B, Arbak S, Yesiladali G, Sut N, Altug T, Ober A. Vitamin E protects against the development of radiation-induced pulmonary fibrosis in rats. Clin Oncol (R Coll Radiol). 2007;19(4):260-4. PMID: 17433970.
  11. Yu R, Sun Y, Cai Q, Li Y, Zhu G. [Effects of thymosin alpha-1 on radiation-induced pneumonitis]. Zhongguo Fei Ai Za Zhi. 2011;14(3):187-93. Chinese. DOI: 10.3779/j.issn.1009-3419.2011.03.02. PMID: 21426658.
  12. Yang HJ, Youn H, Seong KM, Yun YJ, Kim W, Kim YH, Lee JY, Kim CS, Jin YW, Youn B. Psoralidin, a dual inhibitor of COX-2 and 5-LOX, regulates ionizing radiation (IR)-induced pulmonary inflammation. Biochem Pharmacol. 2011;82(5):524-34. DOI: 10.1016/j.bcp.2011.05.027. PMID: 21669192.
  13. Hunter NR, Valdecanas D, Zhongxing L, Milas L, Thames HD, Mason KA. Mitigation and treatment of radiation-induced thoracic injury with a cyclooxygenase-2 inhibitor, celecoxib. Int J Radiation Oncol Biol Phys. 2013;85(2):472-6. DOI:10.1016/j.ijrobp.2012.04.025.
  14. Vlasenko TN, Nazarov VB, Grebenyuk AN. [Modern approaches to pharmacological prophylaxis of radiation injures]. Farmakologiya. 2010;11(8):230-53. Russian.
  15. Vlasenko TN, Nazarov VB, Grebenyuk AN. [Modern approaches to early pharmacotherapy of radiation injures]. Farmakologiya. 2010;11(11):247-62. Russian.
  16. Atamanova OM, Andrianova IE, Tkach VV, Sivuha NI, Chertkov KS, Filimonova GI, Mokievskaya TI, inventors. Institution of biophisics Department of Health RF, assignee. A method for treating acute radiation syndrome in experimental animals. Russian Federation patent RU 2101774 1998 Jan. 10. Int. Cl. G09B23/28. Russian.
  17. Pikalyuk VS, Bessalova YeYu, Tkach VV (jun.), Kriventsov MA, Kisel`ov VV, Shaymardanova LR, authors; Pikalyuk VS, editor: Likvor kak gumoralnaia sreda organizma [CSF as humoral environment of the body]. Simferopol: Arial; 2010. 192 p. Russian.
  18. Zapadnyuk IP, Zapadnyuk VI, Zahariya EA, Zapadnyuk BV. Laboratornye zhivotnye. Razvedenie, soderzhanie, ispolzovanie v eksperimente [Animal testing. Breeding, maintenance, use in the experiment]. 3rd , rev., enl. Kiev: Vischa shkola. Golovnoe Publ., 2013. 383 p. Rus-sian.
  19. Pikalyuk VS, Tkach VV, Kriventsov MA, Shaymardanova LR, Bessalova YeYu, Kisel`ov VV, Zayviy YuP, Leskovskiy AO, inventors. State Institution “Crimea State Medical University named after S. I. Georgievskiy”, assignee. The method of producing native biological preparation of liquor. Ukraine patent UA 65154. 2011 Nov 25. Int. Cl. A61K 35/24 (2006.01), A61k 35/12 (2006.01). Ukrainian.
  20. Korjevskiy DE, Giliarov AV, editors. Osnovi gistologicheskoy tehniki [Basis of histological techniques]. SanktPeterburg: SpetsLit; 2010. 95 p. Russian.
  21. Shalanin VV. [Morphological changes in the lungs in terms of substitution surfactant therapy experimental pneumonia on a background of alcohol intoxication in albino rats]. Reports of morphology. 2001;7(2):245-7. Russian.
  22. Shimkus TS, Nechiporenko GV. [Macro- microscopic changes in the lungs exposed to hypergravity, corrected method of physical protection and pharmacological correction]. Reports of morphology. 2007;2(13):293-300. Russian.
  23. Makarova NV, Trofimec VYa., authors: Statistika v Excel [Statistics in Excel]. Moskow: Finansy i statistika; 2002. 368 p. Russian.
  24. Zhang Yu, Zhang X, Rabbani ZN., Jackson IL., Vujaskovic Z. Oxidative Stress Mediates Radiation Lung Injury by Inducing Apoptosis. Int J Radiat Oncol Biol Phys. 2012;83(2):740-8. DOI: 10.1016/j.ijrobp.2011.08.005. PMCID: PMC3649017. NIHMSID: NIHMS462963.
  25. Osterreicher J, Mokry J, Navrátil L, Knízek J, Vávrová J, Skopek J, Macela A. The alveolar septal thickness and type II pneumocytes number in irradiated lungs, time expression and the effect of pentoxifylline. Acta Medica (Hradec Kralove). 2001;44(1):15-9. PMID: 11367886.
  26. Almeida C, Nagarajan D, Tian J, Leal SW, Wheeler K, Munley M, Blackstock W, Zhao W. The Role of Alveolar Epithelium in Radiation-Induced Lung Injury. PLoS ONE. 2013:8(1):e53628. DOI:10.1371/journal.pone.0053628.
  27. Rübe CE, Uthe D, Schmid KW, Richter KD, Wessel J, Schuck A, Willich N, Rübe C. Dose-depended induction of transforming growth factor-b (TGF-b) in the lung tissue of fibrosis-prone mice after thoracic irradiation. Int. J. Radiation Oncology Biol. Phys. 2000;47(4):1033-42.
  28. Nian-Hua Ding, Jian Jian Li, Lun-Quan Sun. Molecular mechanisms and treatment of radiation-induced lung fibrosis. Current Drug Targets. 2013;14(11):1347-56. PMID: 23909719.
  29. Uzlenkova NE. [Peculiarities of collagen changes in the organs of rats at single x-ray exposure]. Ukr J Radiol. 2008;16(1):46-53. Ukrainian.
  30. Shuo C, Rong W. Expression of angiotensin II and aldosterone in radiation-induced lung injury. Cancer Biol Med. 2012;9(4):254-60. DOI: 10.7497/j.issn.2095-3941.2012.04.006.
  31. Kma L, Gao F, Fish BL, Moulder JE, Jacobs ER, Medhora M. Angiotensin converting enzyme inhibitors mitigate collagen synthesis induced by a single dose of radiation to the whole thorax. J Radiat Res. 2012;53:10-7. DOI:10.1269/jrr.11035.
  32. Cappuccini F, Eldh T, Bruder D, Gereke M, Jastrow H, Schulze-Osthoff K, Fischer U,Köhler D, Stuschke M, Jendrossek V. New insights into the molecular pathology of radiation-induced pneumopathy. Radiother Oncol. 2011;101(1):86-92. DOI: 10.1016/j.radonc.2011.05.064. PMID: 21722981.
  33. Nesterov YyV, Tepliy DL. [Ontogenetic particulars stress-reactive of lungs in connection of lipids metabolism]. Biomeditsinskaya khimiya, 2003;49(5):456-62.

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How to Cite

Shatov, D. V., Pikalyuk, V. S., Shalanin, V. V., & Shimkus, T. S. (2014). The influence of xenogenic cerebrospinal fluid over the parenchyma of rat’s lungs after single total irradiation. Морфологія / Morphologia / Morfologìâ, 8(2), 77–88. https://doi.org/10.26641/1997-9665.2014.2.77-88

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Vol. 8 No. 2 (2014)

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