Influence of a new wound-healing ointment on morphogenesis of full-thickness excision wounds in healthy rats and rats with diabetic state.

Authors

  • L. V. Derymedvid Національний фармацевтичний університет, Харків, Ukraine
  • O. V. Tsulun Національний фармацевтичний університет, Харків, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2014.2.20-29

Keywords:

ointment, morphology, antioxidants, wound process

Abstract

Background. Impaired wound healing is a complication of diabetic state and is a serious medical and social problem. It occurs as a consequence of disequilibrium in reactive oxygen species (ROS) and antioxidant defense system. Therefore local treatment with antioxidants is promising in this type of pathology. Objective. The aim of this study was morphological evaluation of the influence of a new wound-healing ointment containing licorice extract, coenzyme Q10 and dihydroquercetin on the model of natural and delayed wounds in rats. Methods. For morphological evaluation on Day 6 and 12 we took specimens of tissues with wound and surrounding intact skin. Prepared histological slides and stained with haematoxylin, eosin and picrofuchsin by Van Gieson. Results. It was noted that process of excision wound healing in rats with diabetic state had prolonged inflammatory phase. Thus, on the Day 6 of observation during wound-healing in a physiologically healthy rats fibrinoid-necrotic layer in the surface layers of the defect was quite dense, with a limited number of leukocyte cells. Young newly formed granulation tissue in the bottom areas also purified from neutrophil leukocytes. Inflammatory reaction in the boundary to the defect areas of skin was extinguished. And in rats with diabetic state same fibrinoid-necrotic layer was markedly attenuated, full of polymorphonuclear leukocytes, granulation cells also contained many white blood cells in the boundary areas of healthy skin observed exocytosis of epithelial layer, a marked inflammation and destruction of the dermis. All this led to decrease in speed of development and maturation of granulation tissue on the 12th day of observation in these animals compared with physiologically healthy rats. Conclusion. The results showed that the studied ointment optimizes reparative tissue regeneration in the healing of skin excision wounds in rats with pre-modeled diabetogenic state. Nature of reparative processes in these rats generally followed that of the physiologically healthy rats.

References

  1. Tsala DE, Amadou D, Habtemariam S. Natural wound healing and bioactive natural products. Phytopharmacology. 2013;4(3):532-60.
  2. Yakovleva LV, Laryanovska YuB, Tkachova OV. [Morphological study of wound healing effect of “Lipovit” ointment in rats]. Clinical pharmacology. 2003;7(1):50-2. Ukrainian.
  3. Hossam E, Osama MA, Ayman MM, Rasha RA. Limiting prolonged inflammation during proliferation and remodeling phases of wound healing in streptozotocin-induced diabetic rats supplemented with camel undenatured whey protein. BMC Immunology. 2013;14(31):3-13.
  4. Anamika M, Shukla R, Shukla A. Antioxidant status in delayed healing type of wounds. Int J Exp Path. 2000;81:257-63.
  5. Lien YH, Stern R, Fu JC, Siegel R. Inhibition of collagen fibril formation in vitro and subsequent crosslinking by glucose. Science. 1984;225:1489-94.
  6. Tsulun OV, Derymedvid LV, Gaman DV. [Eficacy of a new combines ointment with dihydroquercetin, coenzyme Q10 and licorice extract on UV-erythema in rats]. Problems of ecological and medical genetics and clinical immunology. 2013; 5(119):262-72. Ukrainian.
  7. Shalam Md, Harish MS, Farhna SA. Prevention of dexamethasone- and fructose-induced insulin resistance in rats by SH-01D, a herbal preparation. Indian Journal of Pharmacology. 2013;38(6):419-22.
  8. Stefanov OV. Doklinichne doslidjennia likarskix zasobiv: metodychni recomendatsii [Pre-clinical study of drugs: methodological recommendations]. Avitsena Publ; 2001. 528 p. Ukrainian.
  9. Yakovleva LV, Tkachova OV, Butko YaO, Laryanovska YuB. [Methodical recommendations “Experimental study of new preparations for local treatment of wounds”]. Kharkiv: NUPh Publ; 2013. 52 p. Ukrainian.
  10. Merkulov GA. [Course of pathologohistological thechnics]. Moscow: Medicina; 1969. 424 p. Russian.

Downloads

How to Cite

Derymedvid, L. V., & Tsulun, O. V. (2014). Influence of a new wound-healing ointment on morphogenesis of full-thickness excision wounds in healthy rats and rats with diabetic state. Морфологія / Morphologia / Morfologìâ, 8(2), 20–29. https://doi.org/10.26641/1997-9665.2014.2.20-29

Issue

Vol. 8 No. 2 (2014)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.