Ultrastructure of rat sciatic nerve at the streptozotocin-induced neuropathy conditions under administration of alpha-lipoic acid preparations.

Authors

  • S. M. Dronov ГУ «Днепропетровская медицинская академия МЗ Украины», Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2014.3.17-22

Keywords:

peripheral neuropathy, sciatic nerve, ultrastructural changes, α-lipoic acid, side effects

Abstract

Background. Diabetic polyneuropathy is one of the most common long-term complications of diabetes. Hyperglycemias caused by ischemia and peroxidation of lipids are the presumed cause of diabetic neuropathy. Appointment of alpha-lipoic acid can restore the function of peripheral nerves, preventing the development of autonomic and trophic disorders. Objective. To determine the extent of changes in the ultrastructure of the sciatic nerve in rats with streptozotocin-induced simulated peripheral neuropathy in experimental therapy with α-lipoic acid. Methods. The research was conducted on 22 white rats divided randomly in 3 groups: group 1 – intact animals; group 2 – rats with streptozotocin-induced neuropathy; group 3 – rats with streptozotocin neuropathy + alpha-lipoic acid. Diabetes mellitus was induced in rats by a single intraperitoneal injection of streptozotocin (50 mg/kg). alpha-lipoic acid was administered intraperitoneally and intragastrically once per day during 40 days from the 56th day after streptozotocin injection. Transmission electron microscopy was performed on the 40th day of alpha-lipoic acid administration. Results. Administration of alpha-lipoic acid activates compensatory-adaptive processes in neuronal cells, reduces the severity of violations in the composition of myelinated nerve fibers, contributes to a distinct axonal spruting, causes the reduction of asymmetrically distributed electron density in the lamellar structure of myelin, indicating an increase in the insulation of axons of the sciatic nerve fibers of rats. Conclusion. Experimental studies confirm high neuroprotective potential of alpha-lipoic acid in streptozotocin-induced neuropathy.

References

  1. Vorobyova AA. [Diabetic neuropathy: some aspects of prevalence, pathogenesis and treatment]. Russkii meditsinskii zhurnal. 2009;17(11):748-51. Russian.
  2. Tomlinson DR, Gardiner NJ. Glucose neurotoxicity. Nat Rev Neurosci. 2008 Jan;9(1):36-45. Cited in: PubMed; PMID: 18094705.
  3. Kehrer JP. Cause-effect of oxidative stress and apoptosis. Teratology. 2000 Oct;62(4):235-6. Cited in: PubMed; PMID:10992267.
  4. Ametov AS, Strokov IA, Barinov AN. [Alpha-lipoic acid in the treatment of symptomatic diabetic polyneuropathy]. Farmateka. 2004;11:69-73. Russian.
  5. Korpachev VV, Shcherbak AV. [Thioctic acid: problems and prospects]. Visnyk farmakolohii ta farmatsii. 2003;(3):20-8. Russian.
  6. Stepanov YuM. [Thioctic acid: new opportunities]. Zdorovya Ukrayiny. 2010;(2):51. Russian.
  7. Vorob'eva OV. [Thioctic (alpha-lipoic) acid-spectrum of clinical use]. Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova. 2011;111(10):86-90. Russian.
  8. Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia. 2008 Feb;51(2):216-26. Review. Cited in: PubMed; PMID:18087688.
  9. Calcutt NA. Experimental models of pain-ful diabetic neuropathy. J Neurol Sci. 2004 May;220(1-2):137-9. Cited in: PubMed; PMID:15140623.
  10. Calcutt NA, Jorge MC, Yaksh TL, Chaplan SR. Tactile allodynia and formalin hyperalgesia in streptozotocindiabetic rats: effects of insulin, aldose reductase inhibition and lidocaine. Pain. 1996 Dec;68(2-3):293-9. Cited in: PubMed; PMID: 9121817.
  11. Hunskaar S, Berge OG, Hole K. A modified hotplate test sensitive to mild analgesics. Behav Brain Res. 1986 Aug;21(2):101-8. Cited in: PubMed; PMID: 3755945
  12. Mironov AA, Komissarchik YaYu, Mironov VA. [Methods of electron microscopy in biology and medicine: a guide]. St Petersburg: Nauka; 1994. 400 р. Russian.
  13. Hawkins BT, Davis TP. The blood-brain barrier/neurovascular unit in health and disease. Pharmacol Rev. 2005 Jun;57(2):173-85. Review. Cited in: PubMed; PMID: 15914466.
  14. Kotov SV, Kalinin AP, Rudakov IG. [Diabetic neuropathy]. Moscow: Meditsina; 2000. Р. 45, 139, 150. Russian.
  15. Kempler P. Neuropathies: Pathomechanism, clinical presentation, diagnosis, therapy. Springer; 2002. 208 р.
  16. Bierhaus A, Chevion S, Hofmann M, Quehenherger P. et al. Advanced glycation end product-induced activation of NF–kB is supressed by alpha lipoic in cultured endothelial cells. Diabetes. 1997 Sep;46(9):1481-90. Cited in: PubMed; PMID: 9287050.
  17. Strokov IA, Manukhina EB, Bakhtina LY, Malyshev IY, Zoloev GK, Kazikhanova SI, Ametov AS. The function of endogenous protective systems in patients with insulin-dependent diabetes mellitus and polyneuropathy: effect of antioxidant therapy. Bull Exp Biol Med. 2000 Oct;130(10):986-90. Cited in: PubMed; PMID: 11177301.

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How to Cite

Dronov, S. M. (2014). Ultrastructure of rat sciatic nerve at the streptozotocin-induced neuropathy conditions under administration of alpha-lipoic acid preparations. Морфологія / Morphologia / Morfologìâ, 8(3), 17–22. https://doi.org/10.26641/1997-9665.2014.3.17-22

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Vol. 8 No. 3 (2014)

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