Influence of mother HIV infection on pancreas development of the fetus.

Authors

  • S. A. Sherstiuk Харьковский национальный университет имени В.Н.Каразина, Ukraine

DOI:

https://doi.org/10.26641/1997-9665.2014.4.51-54

Keywords:

pancreas, HIV-infection, deadborn

Abstract

Background. Current stage of HIV infection is characterized by a significant increase in the incidence of infection among women of reproductive age and pregnant women. Consequently it will lead to the complex of problems related to the health of children born to them. Objective. To identify the impact of HIV infection of the mother on the formation of the pancreas of the fetus. Methods. Pancreases were measured and weighed. Histological sections were stained with hematoxylin and eosin, Van Gison and Mallory methods. Immunohistochemical examination was performed using the indirect method of Coons in modifications of M.Brosman. α- and β-cells were detected using monoclonal antibodies to glucagon and insulin. Immunohistochemical study was carried out in the luminescent microscope «Axioskor 40" using the software Biostat.exe. All digital data is processed by methods of mathematical statistics using variations, alternative and correlation analysis. Results. Analyzing the morphometric parameters of the pancreas of stillbirths from HIV-infected mothers, comparing them with the control group we found a massive expansion of the stroma on the background of decreasing both quantitative and qualitative composition of the islets of Langerhans, as well as a violation of the ratio of populations α- and β-cells in the direction of decreasing β-endocrine cells (1: 1.47). The latter ratio is known to play a leading role in the development of the endocrine pancreas dysfunction. Decrease in the density of Langerhans islets due to the growth of the stroma by the newly formed interlobular and intralobular connective tissue, as indicated by stromal-parenchymal ratio of the organ, as well as reducing of the diameter of the island and the number of endocrine cells in it. β-cells of the islets of Langerhans of children of M group had evidences of functional inhibition manifesting in reducing of the cellular diameter and increase of nuclear/cytoplasmic ratio, decrease in the intensity of ß-cells luminescence. Conclusion. Immunohistochemical study of pancreatic stillbirths from HIV-infected mothers showed a decrease in the intensity of luminescence of ß-cells in sections treated with mAb to insulin, which was the strong evidence of their functional inhibition.

References

  1. Dedov II, Peterkova VA. [Pediatric endocrinology]. Moscow: The Universe Publishing; 2006. 600 p. Russian.
  2. Derevtsov VV. [State of health and adaptation-reserve opportunities in neonatal the period of newborn children of mothers with anemias]. Fundamental research. 2010. 8: 10-21 Russian.
  3. Milovanova AP, Savelyev SV. [Intrauterine development of human]. Moscow: ‘WDM’; 2006. 384 p. Russian.
  4. Barchina TG, Moldavskaya AA, Savichev AV, Donskoy MV. [Ultramicroscopic organization of the pancreatic gland under single action of the overloads in the evolutional aspect]. Morphological newsletter. 2008;(3-4): 9-10. Russian.
  5. Stepanov AA, Yatsyk GV, Bombardirova YeP, Belayeva IA, Dragunas TN. [Neonatal endocrine gland functions]. Rossiiskii Pediatricheskii Zhurnal. 2007;(3):55-8. Russian.
  6. Dedov II, Balabolkin MI. [Pathogenesis of diabetes mellitus]. Meditsinskii akademicheskii zhurnal. 2006; 6(3): 3-15 Russian.
  7. Moldavskaia AA, Savischev AV. [Embryogenesis of the pancreas and its topographic and anatomic relations with adjasent organs]. In: [Anatomical and surgical aspects of pediatric gastroenterology: scientific symposium; 2007 May 11; Chernivtsi, Ukraine]. Chernivtsi; 2007. p. 74-5. Russian.
  8. Savichev AV. [The stages and steps in formation and pancreatic gland in man]. Fundamental research. 2010;(9):97-104. Russian.
  9. Martsinovskii VA. [Epidemiological characteristics of HIV infection in children born to HIV-infected women in Ukraine]. Ukrainian Medical Journal. 2006;1:109-13. Ukrainian.
  10. Atramentova LA, Lutevskaya OM. [Statistical methods in biology]. Gorlivka; 2008. 247 p. Ukrainian.
  11. Lapach SK, Chubenko AV, Babich PN. [Statistical methods in biomedical studies using Exel]. Kyiv: Morion; 2001. p. 144-55 Ukrainian.
  12. Sergienko VI, Bondarev IB. [Mathematical statistics in clinical trials]. Moscow: GEOTAR-Meditsina; 2000. p. 256. Russian.
  13. Borovikov VP. [STATISTICA software for students and engineers]. Moscow: ComputerPress; 2001. 301 p. Russian.
  14. Savischev AV, Barkhina TG, Donskoi MV. [Organogenesis of pancreas in the early periods of prenatal human ontogenesis]. In: Proceedings of the conference “Actual problems of morphogenesis in health and disease”. Moscow; 2008. p. 140-3. Russian.
  15. Savischev AV. [The impact of hypoxia on the ultrastructure of pancreatic acinar cells]. In: [Conference dedicated to the 100th birthday of the Honored Scientist of the Russian Federation, acade-mician of the USSR Academy of Medical Sciences Dmitriĭ Arkad'evich Zhdanov. Report and abstracts]. Morfologiia. 2008;133(4):91. Russian. PMID: 19006855.
  16. Evseeva ZP, Sagamonova KIu, Palieva NV, Zamanskaia TA, Markar'ian IV, Melkonov EIu. [Criteria for early diagnosis of placental insufficiency and fetal growth retardation]. Rossiĭskiĭ vestnik akushera-ginekologa. 2008;8(3):12-5. Russian.

Downloads

How to Cite

Sherstiuk, S. A. (2014). Influence of mother HIV infection on pancreas development of the fetus. Морфологія / Morphologia / Morfologìâ, 8(4), 51–54. https://doi.org/10.26641/1997-9665.2014.4.51-54

Issue

Vol. 8 No. 4 (2014)

Section

Статті

License

Copyright (c) 2018 Morphologia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

 The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.
        By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world. 
        The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.