Investigation of models of unilateral pathological processes of breach of local renal blood flow in order to determine changes in the status of the contralateral kidney: immunomorphological aspects.
DOI:
https://doi.org/10.26641/1997-9665.2014.4.43-50Keywords:
blood circulation of kidneys parenchyma, experimental ischemia, infringement of urodynamics, CD3, aSMAAbstract
Background. Morphology of compensatory-adaptive changes and their dynamics in a single remained kidney are still not clarified. Therefore, the study of renal parenchyma lesions in rat models of the most common pathological states has a great practical importance. Objective. To investigate the models of unilateral pathological processes of breach of local renal blood flow (ischemia, infringement of urodynamics) in order to determine changes in the status of the contralateral kidney. Methods. In the experiment on white Wistar rats permanent ischemia was modeled by a fixed bending left kidney artery (15 individuals – group A), acute infringement of urodynamics was modeled by a fixed crossing of the left ureter kidney ( 4 individuals – group B) and both pathological conditions were simulated (5 individuals – group C). Kidneys of 10 relatively healthy rats were used as a control. The primary monoclonal antibodies for CD3 and aSMA (TermoScientific, USA) were used. Results. The main pathological manifestations in the contralateral right "healthy" kidneys was in degenerative changes in epithelial tubule cells, foci of edema and sclerosis around large vessels, interstitial and perivascular inflammatory infiltrates containing CD3+ T-cells (for A II – 10,3±1,2, for B II – 11,9±1,1, for C II – 10,9±0,8). It differs significantly from the control group (p<0,05), where CD3+ T-lymphocytes occurred as single cells of interstitium. Conclusions. Unilateral damage of the kidney due to experimental ischemia, or infringement of urodynamics, or both pathological conditions, affecting local renal blood flow, results in pathological changes in the contralateral "healthy" kidney prior to the nephrectomy and suggests the importance of its medicament support.References
- Lyulko OV, Pidhirny YaM, Barannik SI. [Pathomorphologic changes of kidneys during ischemia]. Medical perspectives. 2006;11(3):16-25. Ukrainian.
- Lul’ko OV, Barannik SI, authors. Zmini mikrocirkulatornogo rusla ta ATF-asnoi aktivnosti parenhimi nirok pri experementalniy ishemii [Changes in microcirculation and ATPase activity in experimental ischemia of the one kidney parenchyma] Urology. 2005;4:22-7. Ukrainian.
- Marchenko TV, Morozov YA, Doletskaya LG, authors. [Functional status of solitary kidney after nephrectomy in living kidney donors of related kidney: is all that simple?] Pochki. 2014;(1):14-7. Russian.
- Petersen RO, Sesterhenn IA, Davis ChJ. Urologic Pathology. 3rd ed. Washington, USA: Lippincott Williams & Wilkins; 2009. 612 p.
- Bostwick DG, Cheng L. Urologic Surgical Pathology. 2nd ed. Washington, USA: MOSBE Elsevier; 2008. 1026 p.
- Fogo AB, Cohen AH, Jennette JCh, Bruijn JA, Colvin RB. Fundamentals of Renal Pathology. New York, USA: Springer; 2006. 221 р.
- Howie AJ. Handbook of Renal Biopsy Pathology. 2nd ed. New York, USA: Springer; 2008. 337 p.
Downloads
How to Cite
Stus, V. P., Barannik, K. S., Shponka, I. S., & Poslavska О. V. (2014). Investigation of models of unilateral pathological processes of breach of local renal blood flow in order to determine changes in the status of the contralateral kidney: immunomorphological aspects. Морфологія / Morphologia / Morfologìâ, 8(4), 43–50. https://doi.org/10.26641/1997-9665.2014.4.43-50
Issue
Section
Статті
License
Copyright (c) 2018 Morphologia
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors reserve the right to authorship of their work and transfer to the Journal the right to the first publication of this work under the terms of a license Creative commons Attribution 4.0 International (CC BY 4.0), which allows other people to freely distribute the published work with a mandatory reference to the authors of the original work and the first publication of the work in this journal.By submitting a manuscript to the editorial office of the Journal ‘Morphologia’ authors agree to transfer the rights to protect and use the manuscript (all supplemental materials, particularly protected objects such as photos, drawings, diagrams, tables, etc.), including the reproduction in the press and distribution via the Internet; translation of the manuscript into any language; export and import of journal copies with the Authors’ article in order to make it available for public. Authors convey the rights mentioned above to the editorial office without any temporal or territorial limitation all over the world.
The Authors guarantee that they have the exclusive rights to use the material transferred to editorial office. Editors are not responsible to third parties for contraventions of warranty given by the Authors. The considered rights are transferred to the editorial office since the moment when the current issue is signed for publishing. Reproduction of materials published in the Journal by other individuals and legal entities is possible only with the consent of Editorial office, with the obligatory indication of the full bibliographic reference of the primary publication. The Authors reserve the right to use the published material, its fragments and parts for teaching materials, oral presentations, dissertation thesis prepararion with obligatory bibliographic citation of the original paper. Electron copy of the published article, downloaded from official journal web-site in .pdf format may be put by authors on the official web-site of their institutions, any other official resources with open access.
